Recombinant milk proteins
Provided herein are compositions and methods for producing recombinant milk proteins, as well as food compositions comprising the same. In aspects, the recombinant milk proteins of the disclosure are recombinant fusion proteins comprising casein and beta-lactoglobulin.
1. A recombinant fusion protein, comprising:
a) β-casein; and
b) β-lactoglobulin.
2. The recombinant fusion protein of claim 1 , further comprising a protease cleavage site.
3. The recombinant fusion protein of claim 1 , further comprising a chymosin cleavage site.
4. A fusion protein, comprising: β-casein and β-lactoglobulin, wherein the β-casein comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 6 and wherein the β-lactoglobulin comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10.
5. The fusion protein of claim 4 , further comprising a protease cleavage site.
6. The fusion protein of claim 4 , further comprising a chymosin cleavage site.
7. A nucleic acid molecule encoding a fusion protein comprising β-casein and β-lactoglobulin, wherein the β-casein comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 6 and wherein the β-lactoglobulin comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10.
8. The nucleic acid molecule of claim 7 , wherein the nucleic acid sequence is codon optimized for expression in a plant.
9. The nucleic acid molecule of claim 8 , wherein the plant is a soybean plant.
10. An expression vector comprising the nucleic acid molecule of claim 7 .
11. A host cell comprising the expression vector of claim 10 .
12. The host cell of claim 11 , wherein the host cell is selected from the group consisting of plant cells, bacterial cells, fungal cells, and mammalian cells.
13. The host cell of claim 11 , wherein the host cell is a plant cell.
14. A plant stably transformed with the nucleic acid molecule of claim 7 .
15. The plant of claim 14 , wherein the plant is a monocot selected from the group consisting of turf grass, maize, rice, oat, wheat, barley, sorghum, orchid, iris, lily, onion, palm, and duckweed.
16. The plant of claim 14 , wherein the plant is a dicot selected from the group consisting of Arabidopsis, tobacco, tomato, potato, sweet potato, cassava, alfalfa, lima bean, pea, chick pea, soybean, carrot, strawberry, lettuce, oak, maple, walnut, rose, mint squash, daisy, quinoa, buckwheat, mung bean, cow pea, lentil, lupin, peanut, fava bean, French beans, mustard, and cactus.
17. The plant of claim 14 , wherein the plant is a soybean plant.
18. A food composition, comprising: a fusion protein comprising β-casein and β-lactoglobulin.
19. The food composition of claim 18 , wherein the food composition is a solid.
20. The food composition of claim 18 , wherein the food composition is a liquid.
21. The food composition of claim 18 , wherein the food composition is a powder.
22. The food composition of claim 18 , wherein the food composition is selected from the group consisting of: cheese, processed cheese product, yogurt, fermented dairy product, directly acidified counterpart of fermented dairy product, cottage cheese, dressing, frozen dairy product, frozen dessert, dessert, baked good, topping, icing, filling, low-fat spread, dairy-based dry mix, soup, sauce, salad dressing, geriatric nutrition, cream, creamer, analog dairy product, follow-up formula, baby formula, infant formula, milk, dairy beverage, acid dairy drink, smoothie, milk tea, butter, margarine, butter alternative, growing up milk, low-lactose product, low-lactose beverage, medical and clinical nutrition product, protein bar, nutrition bar, sport beverage, confection, meat product, analog meat product, meal replacement beverage, weight management food and beverage, dairy product, cultured buttermilk, sour cream, yogurt, skyr, leben, lassi, kefir, powder containing a milk protein, and low-lactose product.
23. The food composition of claim 18 , wherein the food composition is a dairy product.
24. The food composition of claim 18 , wherein the food composition is an analog dairy product.
25. The food composition of claim 18 , wherein the food composition is a low lactose product.
26. The food composition of claim 18 , wherein the food composition is a milk.
27. The food composition of claim 18 , wherein the food composition is a cheese.
28. The food composition of claim 18 , wherein the food composition is fermented.