IP Library Granted Patent US 11,214,781
Granted Patent B2
US 11,214,781 · App. 17/374,628 · Granted Jan 4, 2022

Engineered enzyme

Inventors: Juhan Kim (Boulder, CO); Benjamin Mijts (Boulder, CO)
Assignee: Inscripta, Inc.
C12N9/22
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 11,214,781
App. No.
17/374,628
Granted
Jan 4, 2022
Kind
B2
Abstract

The present disclosure provides a novel catalytically inactive MAD7 nuclease (dMAD7) that retains the ability to bind DNA in a sequence-specific manner. The MAD7 nuclease from which the dMAD7 has been derived was isolated from Eubacterium rectale.

Claims (3)

1. A catalytically inactive CRISPR nuclease obtained from Eubacterium rectale MAD7 (dMAD7) having the amino acid sequence SEQ ID NO: 2 that retains the ability for sequence specific binding a bacteria genome.

2. The catalytically inactive CRISPR nuclease of claim 1 for sequence-specific binding a yeast genome.

3. The catalytically inactive CRISPR nuclease of claim 1 for sequence-specific binding an isolated mammalian cell genome.

Assignments (3)
MERGER AND CHANGE OF NAME Recorded Sep 15, 2025
From: INSCRIPTA, INC.,; MANUS INSCRIPTA, INC.
To: MANUS INSCRIPTA, INC.
Reel/Frame 072252/0203 →
SECURITY INTEREST Recorded Sep 8, 2025
From: MANUS BIO INC.; STO.PERU I LLC; STO.PERU II LLC; MANUS INTERMEDIATE INC.; MANUS INSCRIPTA, INC.
To: SYMBIOTIC CAPITAL AGENCY LLC, AS ADMINISTRATIVE AND COLLATERAL AGENT
Reel/Frame 072836/0255 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 1, 2021
From: KIM, JUHAN; MIJTS, BENJAMIN
To: INSCRIPTA, INC.
Reel/Frame 057359/0948 →
Continuity (6)
Continuation In Part 16953253 · Nov 19, 2020
Continuation 16844079 · Apr 9, 2020
Continuation 16798315 · Feb 22, 2020
Continuation 16658948 · Oct 21, 2019
Provisional Application 62748668 · Oct 22, 2018
Related Publication 20210340513A1 · Nov 4, 2021
Cited By (1)
US 12,668,819