Granted Patent
B2
US 12,449,424 · App. 17/413,548 · Granted Oct 21, 2025
In vitro method for detecting avian intestinal dysbiosis
Inventors:
Stefan Pelzer (Gütersloh, DE); Monika Flügel (Steinhagen, DE); Sarah Hark (Gütersloh, DE); Evy Goossens (Wachtebeke, BE); Filip Van Immerseel (Eke, BE); Richard Ducatelle (Wortegem-Petegem, BE); Lieven Van Meulebroek (Zottegem, BE); Lynn Vanhaecke (Balegem, BE)
Assignee:
Evonik Operations GmbH
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Abstract
The invention pertains to an in vitro method for detecting avian intestinal dysbiosis, the method comprising determining the presence and/or level of isoleucyl-arginine (C 12 H 25 O 3 N 5 ) or isomers thereof in avian sample material wherein the presence and/or an increased level of isoleucyl-arginine (C 12 H 25 O 3 N 5 ) or isomers thereof in comparison to a non-affected control is indicative for avian intestinal dysbiosis.
Claims (19)
1. An in vitro method for detecting avian intestinal dysbiosis, comprising:
a) determining the presence or level of isoleucyl-arginine or isomers thereof in avian sample material, wherein the presence or an increased level of isoleucyl-arginine or isomers thereof in comparison to a non-affected control animal is indicative for avian intestinal dysbiosis, wherein the presence or level of isoleucyl-arginine or isomers thereof are detected or quantified via LC-MS, an enzyme assay, or LC combined with pre- or post-column derivatization and fluorescence or UV detection; and
b) administering probiotic agents, prebiotic agents, botanicals, organic/fatty acids, zeolites, bacteriophages or bacteriolytic enzymes or a combination thereof to animals exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof in comparison to a control animal.
2. The method of claim 1 , wherein the avian sample material is, of comprises, avian excremental material.
3. The method of claim 1 , wherein the avian sample material is, of comprises, avian feces.
4. The method of claim 1 , wherein the avian sample material is, of comprises, pooled avian feces deriving from an avian flock.
5. The method of claim 1 , wherein the isoleucyl-arginine or isomers thereof are detected or quantified via LC-MS.
6. The method of claim 1 , wherein the isoleucyl-arginine or isomers thereof are detected or quantified via an enzyme assay.
7. The method of claim 1 , wherein the isoleucyl-arginine or isomers thereof are detected or quantified via LC combined with pre- or post-column derivatization and fluorescence or UV detection.
8. The method of claim 4 , wherein the isoleucyl-arginine or isomers thereof are detected or quantified via LC-MS.
9. The method of claim 4 , wherein the isoleucyl-arginine or isomers thereof are detected or quantified via an enzyme assay.
10. The method of claim 4 , wherein the isoleucyl-arginine or isomers thereof are detected or quantified via LC combined with pre- or post-column derivatization and fluorescence or UV detection.
11. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is administered a probiotic agent in its feed or water.
12. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is administered a prebiotic in its feed or water.
13. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is administered a botanical in its feed or water.
14. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is administered organic acid in its feed or water.
15. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is treated by administering a fatty acid to the animal in its feed or water.
16. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is administered a zeolite in its feed or water.
17. The method of claim 1 , wherein, in step b), said animal exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof is administered a bacteriophage or a bacteriolytic enzyme in its feed or water.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 23, 2021
From: PELZER, STEFAN; FLÜGEL, MONIKA; HARK, SARAH; GOOSSENS, EVY; VAN IMMERSEEL, FILIP; DUCATELLE, RICHARD; VAN MEULEBROEK, LIEVEN; VANHAECKE, LYNN
To: EVONIK OPERATIONS GMBH
Reel/Frame 056956/0708 →
Priority Claims (1)
EP 18212599
· Dec 14, 2018
· regional
Continuity (1)
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