Methods and uses for metabolic profiling for
View Patent ↗Embodiments include methods for generating a metabolite profile of a stool sample and methods of assessing the status of a subject using the metabolic profile derived from a stool sample.
1. A method of treating a subject having a Clostridium difficile infection (CDI) comprising:
measuring levels of gamma-aminobutyrate (GABA), ammonia, or GABA and ammonia, in a stool sample from a subject; and
administering a treatment for CDI to the subject if the levels of the one or more biomarkers are elevated compared to a reference level.
2. The method of claim 1 , wherein the treatment of CDI includes administering metronidazole, vancomycin, fidaxomicin, rifampicin, rifaximin, nitazoxanide, rifabutin, or combinations thereof.
3. The method of claim 1 , wherein the treatment of CDI includes administering a probiotic therapy.
4. The method of claim 1 , wherein the treatment of CDI includes administering phytic acid or derivatives thereof.
5. A method of treating a subject having a recurrent Clostridium difficile infection (CDI) comprising:
distinguishing a subject having recurrent Clostridium difficile infection from a subject having a non-recurrent Clostridium difficile infection comprising measuring levels of one or more biomarkers selected from ammonia or gamma-aminobutyrate (GABA) in a stool sample and identifying a subject having a recurrent CDI if one or more biomarkers are elevated more than 200% compared to a non-infected control level; and
administering a treatment for CDI to the subjects identified as having recurrent CDI.
6. The method of claim 5 , wherein the biological sample is a stool sample.