Granted Patent
B2
US 12,630,484 · App. 17/459,019 · Granted May 19, 2026
Methods for promoting plant health using free enzymes and microorganisms that overexpress enzymes
Assignee:
Spogen Biotech Inc.
C05F17/20A01N63/50C05B15/00C05C9/00C05F11/08C05G3/60C07K14/415C12N1/20C12N9/78C12N15/8237C12N15/8249C12N15/8279C12Y302/01C12Y305/99007C12Y402/0202Y02A40/22Y02P60/21
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Abstract
Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.
Claims (23)
1 . A method for stimulating plant growth and/or promoting plant health comprising applying a free enzyme to a plant seed, wherein the enzyme is selected from:
a phospholipase comprising an amino acid sequence having at least 95% identity to any one of SEQ ID NOs: 18, 19, 115, 116 or 117;
a lipase comprising an amino acid sequence having at least 95% identity to any one of SEQ ID NOs: 118, 119 or 120;
a xylanase comprising an amino acid sequence having at least 95% identity to any one of SEQ ID NOs: 25, 121, or 122;
a xylosidase comprising an amino acid sequence having at least 95% identity to SEQ ID NO: 123; or
a chitosanase comprising an amino acid sequence having at least 95% identity to SEQ ID NO: 124; and
wherein a plant grown from the plant seed exhibits increased height or yield as compared to a plant grown from a control seed under the same conditions.
2 . The method of claim 1 , wherein applying the enzyme to the plant seed comprises: (a) applying the enzyme to the plant seed at the time of planting; or
(b) coating the plant seed with the enzyme.
3 . The method of claim 2 , wherein the method comprises coating the plant seed with a seed coating formulation comprising:
the enzyme; and
an agriculturally acceptable carrier.
4 . The method of claim 2 , wherein the method comprises coating the plant seed with the enzyme.
5 . The method of claim 1 , wherein the enzyme comprises the phospholipase.
6 . The method of claim 5 , wherein:
the phospholipase comprises an amino acid sequence having at least 98% identity to any one of SEQ ID NOs: 18, 19, 115, 116 or 117.
7 . The method of claim 1 , wherein the enzyme comprises:
a crude cell extract containing the enzyme;
a partially purified enzyme;
a substantially purified enzyme; or
an enzyme that is immobilized on a matrix or support.
8 . The method of claim 1 , wherein the wherein:
the xylosidase comprises an amino acid sequence having at least 98% identity to SEQ ID NO: 123.
Assignments (2)
SECURITY INTEREST
Recorded Jan 23, 2023
From: SPOGEN BIOTECH INC; ELEMENTAL ENZYMES AG AND TURF, LLC; THOMPSON, BRIAN M; THOMPSON, KATIE C
To: GENISYS CREDIT UNION
Reel/Frame 062458/0476 →
SECURITY INTEREST
Recorded Jan 23, 2023
From: SPOGEN BIOTECH INC; ELEMENTAL ENZYMES AG AND TURF, LLC; THOMPSON, BRIAN M; THOMPSON, KATIE C
To: GENISYS CREDIT UNION
Reel/Frame 062458/0544 →
Continuity (3)
Provisional Application
62309426
· Mar 16, 2016
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