Pseudomonas strains and their metabolites to control plant diseases
View Patent ↗The present disclosure concerns methods of using novel bacterial strains of 0617-T307, 0917-T305, 0917-T306, 0917-T307, 0118-T319, 0318-T327, and 0418-T328, the cell broth and novel metabolites produced from the bacterial strains, that can inhibit the growth of a variety of microbial species for a variety of crops and fungal pathogens. The methods include use of novel, potent antimicrobial metabolites produced from the strains corresponding to compounds having Formulas (I), (II), and (III):
1. A method of controlling a crop disease, comprising the steps of
producing an agricultural composition comprising a Pseudomonas bacterial metabolite as Formula (I)
and
applying said agricultural composition to a crop to inhibit the growth of pathogenic microorganisms.
2. The method of claim 1 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, Citrus Greening Disease, Zebra chip disease, and bacterial leaf streak.
3. The method of claim 1 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Xanthomonas citri pv. citri, Candidatus Liberibacter asiaticus, Liberibacter crescens, Ca. Liberibacter americanus, Ca. Liberibacter africanus , and Ca. Liberibacter solanacearum.
4. The method according to claim 1 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae, plant species of Solanaceae, plant species of Cucurbitaceae a stone fruit and a pome fruit.
5. A method of controlling a fungal pathogen on a crop, comprising the steps of
producing an agricultural composition comprising a Pseudomonas bacterial metabolite as Formula (I)
and
applying said agricultural composition to crops to inhibit the growth of the fungal pathogen.
6. The method of claim 5 , wherein the fungal pathogen is selected from the group consisting of Septoria tritici, Colletotrichum dematium, Fusarium oxysporum f. sp. Melonsis , and Magnaporthe oryzae.
7. The method according to claim 5 , wherein the crop is selected from the group consisting of apples, peaches, vines, berry crops, wheat, barley, tomato, sweet potato, melons, legumes, bananas and rice.
8. A method of controlling a fungal crop disease, comprising:
applying an agricultural composition comprising between about 1.0×10 5 and 1.0×10 9 cfu per mL Pseudomonas bacteria to a crop to inhibit the growth of a fungal pathogen.
9. The method of claim 8 , wherein the Pseudomonas bacteria is selected from the group consisting of Pseudomonas soil 0617-T307 (Accession No. PTA-126796), Pseudomonas soil 0917-T305 (Accession No. PTA-126797), Pseudomonas soil 0917-T306 (Accession No. PTA-126798), Pseudomonas soil 0917-T307 (Accession No. PTA-126799), Pseudomonas mosselii 0118-T319 (Accession No. PTA-126800), Pseudomonas mosselii 0318-T327 (Accession No. PTA-126801), and Pseudomonas mosselii 0418-T328 (Accession No. PTA-126802).
10. The method according to claim 9 , wherein the composition comprises between about 5.0×10 7 and 2.0×10 8 cfu per mL Pseudomonas bacteria.
11. The method of claim 8 , wherein the fungal pathogen is selected from the group consisting of Septoria tritici, Colletotrichum dematium, Fusarium oxysporum f. sp. Melonsis , and Magnaporthe oryzae.
12. The method according to claim 8 , wherein the crop is selected from the group consisting of apples, peaches, vines, berry crops, wheat, barley, tomato, sweet potato, melons, legumes, bananas and rice.