Modulated nutritional quality traits in seeds
This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.
1. A method of increasing crude fat content of a seed produced by a wheat plant, comprising mechanically or manually inoculating a wheat plant seed with a treatment formulation comprising an agriculturally compatible carrier and a purified bacterial population that comprises a seed bacterial endophyte of the genus Enterobacter that is heterologous to the wheat plant seed and comprises a polynucleotide sequence having at least 99% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 62, wherein the endophyte is heterologously disposed to the wheat plant seed in an amount effective to colonize a wheat plant germinated from the inoculated wheat plant seed, wherein a seed obtained from the colonized wheat plant exhibits an increase in crude fat content as compared to a reference wheat plant seed obtained from a reference wheat plant lacking the colonized endophyte of the genus Enterobacter , and wherein said reference wheat plant is germinated from a reference wheat plant seed lacking the colonized endophyte of the genus Enterobacter comprising the polynucleotide sequence having at least 99% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 62.
2. The method of claim 1 , wherein the seed bacterial endophyte comprises a polynucleotide sequence having 100% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 62.
3. The method of claim 1 , wherein the agriculturally compatible carrier comprises a solid carrier, a liquid carrier, or both.
4. The method of claim 1 , wherein the treatment formulation comprises one or more of the following: a surfactant, a buffer, a tackifier, a microbial stabilizer, a fungicide, an anticomplex agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, or combinations thereof.
5. The method of claim 1 , wherein the method further comprises drying the wheat plant seed following the mechanical or manual inoculation.