Vaccine delivery systems using yeast cell wall particles
The present invention generally relates to compositions and methods for delivering a vaccine. The compositions and methods disclosed herein are particularly useful in making prophylactic and therapeutic vaccines.
1. A vaccine comprising (i) a yeast cell wall particle (YCWP), (ii) a viral antigen or bacterial antigen loaded into the yeast cell wall particle, and (ii) a silicate, wherein the YCWP is modified by capping with the silicate, wherein the viral antigen or bacterial antigen is not a tumor lysate, wherein the yeast cell wall particle is a hollow, porous structure, and wherein the vaccine does not comprise a cell of monocytic origin.
2. The vaccine of claim 1 , wherein the silicate is tetraethylorthosilicate, tetramethylorthosilicate, tetrapropylorthosilicate, or tetrabutylorthosilicate.
3. The vaccine of claim 1 , wherein the silicate comprises an organic moiety attached to each of the four oxygen compounds of an orthosilicate.
4. A method of treating an infectious disease, comprising administering to a subject the vaccine of claim 1 .
5. A method for producing a yeast cell wall particle loaded with a biological material and capped with a silicate, comprising:
(i) loading a biological material into a yeast cell wall particle to produce a loaded particle wherein the biological material comprises a protein or a fragment thereof, nucleic acid, carbohydrate, antigen or a combination thereof, and wherein the biological material is viral antigen, or bacterial antigen;
(ii) capping the loaded particle with a silicate; and
(iii) freeze-drying the capped, loaded particle.