Tumoricidal and antimicrobial compositions and methods
Provided herein are pharmaceutical compositions comprising tumoricidal and/or antimicrobial components isolated from the supernatant of NK-92 cell medium and methods of using the compositions for killing cancer cells.
1. A composition comprising isolated exosomes or microvesicles from NK-92 cells cultured in a medium supplemented with at least one of a nutrient, a cytokine, or a growth factor, wherein the isolated exosomes or microvesicles are anti-bacterial and anti-viral.
2. The composition of claim 1 , wherein the medium comprises a cytokine and the cytokine is selected from the group consisting of IL-2, IL-15, interferon gamma and combinations thereof.
3. The composition of claim 1 , wherein the medium comprises a nutrient.
4. The composition of claim 1 , wherein the medium comprises a growth factor.
5. The composition of claim 1 , wherein the composition further comprises a cytokine, chemotherapy, cell-based therapy, or antibody therapy.
6. The composition of claim 1 , wherein the medium comprises L-asparagine, L-glutamine, L-serine or combinations thereof.
7. The composition of claim 1 , wherein the isolated exosomes or microvesicles are anti-bacterial.
8. The composition of claim 1 , wherein the isolated exosomes or microvesicles are anti-viral.