Compositions of beta-glucuronidase enzyme blends with enhanced enzymatic activity and methods of preparation thereof
Blends of enzymes of the same enzyme class (same EC number), such as beta-glucuronidase enzyme blends, are provided that exhibit synergistic levels of activity across a range of substrates as compared to the activity levels of each enzyme in the blend individually. The blends also may exhibit a greater effective substrate range, as well as greater effective pH and/or temperature ranges as compared to single enzyme preparations. Predictive methods for determining optimal enzyme blends, methods for preparing enzyme blends, enzyme blend compositions, enzyme blend formulations and methods of using such enzyme blends are provided.
1. A composition comprising a blend of Eubacterium eligens beta-glucuronidase (EeGUS) enzyme and a chimeric beta-glucuronidase enzyme Rxn3, wherein EeGUS comprises the amino acid sequence shown in SEQ ID NO: 10 and Rxn3 comprises the amino acid sequence shown in SEQ II) NO: 19.
2. A formulation comprising the composition of claim 1 , wherein the formulation comprises the blend of EeGUS and Rxn 3 and at least one excipient.
3. The formulation of claim 2 , which is an aqueous formulation.
4. The formulation of claim 2 , which is a lyophilized formulation.
5. The formulation of claim 2 , wherein the at least one excipient is selected from the group consisting of water, salts, buffers, sugars and amino acids.
6. The formulation of claim 2 , which is free of polymers and detergents.
7. The formulation of claim 2 , wherein each enzyme in the blend is present at a concentration of at least 0.1 mg/mL.
8. A packaged formulation comprising the formulation of claim 2 and a container.
9. The composition of claim 1 , wherein the blend comprises 70%-90% EeGUS and 10%-30% Rxn3.
10. The composition of claim 1 , wherein the blend comprises 70%-80% EeGUS and 20%-30% Rxn3.
11. The composition of claim 1 , wherein the blend comprises 70% EeGUS and 30% Rxn3.
12. The composition of claim 1 , wherein the blend comprises 80% EeGUS and 20% Rxn3.