IP Library Granted Patent US 12,305,215
Granted Patent B2
US 12,305,215 · App. 17/571,209 · Granted May 20, 2025

Microbiomes and methods for producing medium-chain fatty acids from organic substrates

Inventors: Timothy James Donohue (Middleton, WI); Matthew Scarborough (Madison, WI); Daniel Noguera (Madison, WI)
Assignee: Wisconsin Alumni Research Foundation
C12P7/6409C12N1/205C12P2203/00C12R2001/225
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Quick Facts
Patent No.
US 12,305,215
App. No.
17/571,209
Granted
May 20, 2025
Kind
B2
Abstract

Microbiome compositions and uses thereof. The microbiome compositions include a set of microbes. The sets of microbes contain members of Lactobacillaceae, Eubacteriaceae, Lachnospiraceae , and Coriobacteriaceae . The number of individual physical microbes in the set constitutes a certain percentage of the total number of individual physical microbes in the microbiome composition. The microbiome compositions can be used for producing medium-chain fatty acids from organic substrates through anaerobic fermentation in a medium. The medium can include lignocellulosic stillage.

Claims (20)

1. A microbiome composition comprising a set of microbes, wherein the microbes in the set consist of members of Lachnospiraceae, Eubacteriaceae, Coriobacteriaceae , and Lactobacillusceae , wherein the number of individual physical microbes in the set constitutes at least 60% of the total number of individual physical microbes in the microbiome composition, wherein the members of Lachnospiraceae constitute at least 40% of the total number of individual physical microbes in the microbiome composition, the members of Eubacteriaceae constitute at least 2% of the total number of individual physical microbes in the microbiome composition, the members of Coriobacteriaceae constitute at least 3% of the total number of individual physical microbes in the microbiome composition, and the members of Lactobacilluseae constitute at least 7% of the total number of individual physical microbes in the microbiome composition.

2. The microbiome composition of claim 1 , wherein one or more of the members of Lachnospiraceae are members of a genus selected from the group consisting of Roseburia and Shuttleworthia.

3. The microbiome composition of claim 1 , wherein one or more of the members of Eubacteriaceae are members of Pseudoramibacter.

4. The microbiome composition of claim 1 , wherein one or more of the members of Coriobacteriaceae are members of a genus selected from the group consisting of Olsenella and Atopobium.

5. The microbiome composition of claim 1 , wherein one or more of the members of Lactobacilliae are members of Lactobacillus.

6. The microbiome composition of claim 1 , wherein the number of individual physical microbes in the set constitutes at least 85% of the total number of individual physical microbes in the microbiome composition.

7. The microbiome composition of claim 1 , wherein less than 1% of the number of individual physical microbes in the microbiome composition are members of Ethanoligenens, Desulfitobacterium, Clostridium, Propionibacterium, Bifidobacterium, Ruminococcaceae , and Bifidobacteriaceae.

8. The microbiome composition of claim 1 , wherein one or more of the members of Lachnospiraceae are members of a genus selected from the group consisting of Roseburia and Shuttleworthia , one or more of the members of Eubacteriaceae are members of Pseudoramibacter , one or more of the members of Coriobacteriaceae are members of a genus selected from the group consisting of Olsenella and Atopobium , and one or more of the members of Lactobacillus are members of Lactobacillus.

9. The microbiome composition of claim 8 , wherein the number of individual physical microbes in the set constitutes at least 85% of the total number of individual physical microbes in the microbiome composition.

10. The microbiome composition of claim 9 , wherein less than 1% of the number of individual physical microbes in the microbiome composition are members of Ethanoligenens, Desulfitobacterium, Clostridium, Propionibacterium, Bifidobacterium , Ruminococcaceae , and Bifidobacteriaceae.

11. The microbiome composition of claim 8 , wherein less than 1% of the number of individual physical microbes in the microbiome composition are members of Ethanoligenens, Desulfitobacterium, Clostridium, Propionibacterium, Bifidobacterium, Ruminococcaceae , and Bifidobacteriaceae.

12. The microbiome composition of claim 1 , wherein the number of individual physical microbes in the set constitutes at least 85% of the total number of individual physical microbes in the microbiome composition, and wherein less than 1% of the number of individual physical microbes in the microbiome composition are members of Ethanoligenens, Desulfitobacterium, Clostridium, Propionibacterium, Bifidobacterium, Ruminococcaceae , and Bifidobacteriaceae.

13. The microbiome composition of claim 1 , wherein each of the members of Lachnospiraceae in the set are members of a genus selected from the group consisting of Roseburia and Shuttleworthia , each of the members of Eubacteriaceae in the set are members of Pseudoramibacter , each of the members of Coriobacteriaceae in the set are members of a genus selected from the group consisting of Olsenella and Atopobium , and each of the members of Lactobacilloae in the set are members of Lactobacillus.

14. The microbiome composition of claim 13 , wherein the number of individual physical microbes in the set constitutes at least 85% of the total number of individual physical microbes in the microbiome composition.

15. The microbiome composition of claim 14 , wherein less than 1% of the number of individual physical microbes in the microbiome composition are members of Ethanoligenens, Desulfitobacterium, Clostridium, Propionibacterium, Bifidobacterium, Ruminococcaceae , and Bifidobacteriaceae.

16. A method of producing medium-chain fatty acids from an organic substrate comprising anaerobically fermenting the organic substrate for a time sufficient to produce medium-chain fatty acids from the organic substrate with the microbiome composition of claim 1 .

17. The method of claim 16 , wherein the organic substrate comprises a component selected from the group consisting of xylose, complex carbohydrates, and glycerol.

18. The method of claim 16 , wherein the organic substrate comprises a lignocellulosic stillage.

19. The method of claim 16 , wherein the fermenting is performed at a pH of about 5 to about 6.5.

20. The method of claim 16 , wherein the fermenting is performed without the addition of ethanol and wherein the fermenting does not produce methane.

Assignments (2)
CONFIRMATORY LICENSE Recorded May 18, 2022
From: UNIVERSITY OF WISCONSIN-MADISON
To: UNITED STATES DEPARTMENT OF ENERGY
Reel/Frame 060104/0941 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 15, 2022
From: DONOHUE, TIMOTHY; SCARBOROUGH, MATTHEW; NOGUERA, DANIEL
To: WISCONSIN ALUMNI RESEARCH FOUNDATION
Reel/Frame 059011/0512 →
Continuity (5)
Continuation 16433639 · Jun 6, 2019
Provisional Application 62846378 · May 10, 2019
Provisional Application 62697249 · Jul 12, 2018
Provisional Application 62696677 · Jul 11, 2018
Related Publication 20220127651A1 · Apr 28, 2022
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