Flow cytometers including fiber optic light collectors, and methods of use thereof
Flow cytometers having fiber optic light collectors are provided. Aspects of the subject flow cytometers involve a flow cell configured to transport particles in a core stream, a light source configured to produce a beam for irradiating the particles in the core stream and a fiber optic light collector for receiving particle-modulated light from the irradiated particles and conveying collected light to a detector. In some cases, the fiber optic light collector is optically aligned to the core stream and includes a collection surface having opposing edges running parallel to the direction of the core stream. Methods of analyzing a sample are also provided.
1. A flow cytometer comprising:
a flow cell configured to transport particles in a core stream;
multiple spatially separated lasers configured to irradiate the particles in the core stream with multiplexed beams; and
a fiber optic light collector for receiving particle-modulated light from the irradiated particles and conveying collected light to a detector, wherein the fiber optic light collector is optically aligned to the core stream and comprises a collection surface having opposing edges running parallel to the direction of the core stream.
2. The flow cytometer according to claim 1 , wherein the collection surface of the fiber optic light collector is rectangular.
3. The flow cytometer according to claim 2 , wherein the collection surface of the fiber optic light collector is square.
4. The flow cytometer according to claim 1 , further comprising an objective lens positioned between the flow cell and the fiber optic light collector, wherein the objective lens is configured to focus particle-modulated light from the core stream.
5. The flow cytometer according to claim 4 , further comprising a refractive optical element positioned between the objective lens and the and the fiber optic light collector, wherein the refractive optical element is configured to direct objective lens focused light onto the collection surface of the fiber optic light collector.
6. The flow cytometer according to claim 5 , wherein the refractive optical element is a prism.
7. The flow cytometer according to claim 1 , further comprising a fiber optic light conveyor configured to convey light from each of the multiple lasers to the flow cell.
8. The flow cytometer according to claim 7 , comprising a single fiber optic light conveyor and a single fiber optic light collector.
9. The flow cytometer according to claim 8 , wherein the single fiber optic light conveyor is configured to convey multiplexed beams from the multiple lasers to the flow cell.
10. The flow cytometer according to claim 1 , further comprising a detection module operably connected to the fiber optic light collector, wherein the detection module comprises a detector.
11. The flow cytometer according to claim 10 , wherein the detection module is configured to demultiplex light collected by the fiber optic light collector.
12. The flow cytometer according to claim 10 , wherein the detection module comprises a plurality of detectors operably connected to the fiber optic light collector.
13. A method of analyzing a sample, the method comprising:
(a) introducing the sample into a flow cytometer comprising:
a flow cell configured to transport particles in a core stream;
multiple spatially separated lasers configured to irradiate the particles in the core stream with multiplexed beams; and
a fiber optic light collector for receiving particle-modulated light from the irradiated particles and conveying collected light to a detector, wherein the fiber optic light collector is optically aligned to the core stream and comprises a collection surface having opposing edges running parallel to the direction of the core stream; and
(b) detecting particle-modulated light collected by the fiber optic light collector to analyze the sample.
14. The method according to claim 13 , wherein the collection surface of the fiber optic light collector is rectangular.
15. The method according to claim 14 , wherein the collection surface of the fiber optic light collector is square.
16. A method of assembling a flow cytometer, the method comprising:
(a) positioning a fiber optic light collector within a flow cytometer, wherein:
the flow cytometer comprises:
a flow cell configured to transport particles in a core stream; and
multiple spatially separated lasers configured to irradiate the particles in the core stream with multiplexed beams; and
the fiber optic light collector is configured to receive particle-modulated light from the irradiated particles and convey collected light to a detector, wherein the fiber optic light collector comprises a collection surface having opposing edges running parallel to the direction of the core stream; and
(b) optically aligning the fiber optic light collector and the core stream such that particle-modulated light is collected.
17. The method according to claim 16 , wherein the collection surface of the fiber optic light collector is rectangular.
18. The method according to claim 17 , wherein the collection surface of the fiber optic light collector is square.