Compositions and methods for improving crop yields through trait stacking
The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits and yield relative to a control plant, such as increased ear area, increased ear volume, increased single kernel weight, increased ear fresh weight, increased ear diameter, increased ear length, increased yield, increased kernels per ear, decreased ear tip void, and decreased ear void. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptides and a transgene encoding one or more MADS-box polypeptides, and can also have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.
1. A modified corn plant or a plant part thereof comprising 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, 2) a second recombinant expression cassette comprising a first DNA sequence encoding a CONSTANS-like (COL) polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, and 3) a third recombinant expression cassette comprising a second DNA sequence encoding a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes.
2. The modified corn plant or plant part thereof of claim 1 , wherein the first, second and third recombinant expression cassettes are stably integrated into the genome of the corn plant or plant part thereof.
3. The modified corn plant or plant part thereof of claim 1 , wherein the transcribable DNA sequence comprises a sequence that is at least 90% identical or complementary to at least 15 consecutive nucleotides of SEQ ID NO: 39, 53, or 55.
4. The modified corn plant or plant part thereof claim 1 , wherein the first DNA sequence of the second recombinant expression cassette comprises a sequence that is at least 90% identical to SEQ ID NO: 169.
5. The modified corn plant or plant part thereof of claim 1 , wherein the second DNA sequence of the third recombinant expression cassette comprises a sequence that is at least 90% identical to SEQ ID NO: 177.
6. The modified corn plant or plant part thereof of claim 1 , wherein the expression level of an endogenous GA20 oxidase gene is reduced or eliminated in the modified corn plant or plant part thereof.
7. The modified corn plant or plant part thereof of claim 1 , wherein the transcribable DNA sequence encoding the non-coding RNA for suppression of the one or more GA20 oxidase genes is operably linked to a heterologous plant-expressible promoter.
8. The modified corn plant or plant part thereof of claim 7 , wherein the heterologous plant-expressible promoter:
i. is a vascular promoter;
ii. is selected from the group consisting of a sucrose synthase promoter, a sucrose transporter promoter, a Sh1 promoter, Commelina yellow mottle virus (CoYMV) promoter, a wheat dwarf geminivirus (WDV) large intergenic region (LIR) promoter, a maize streak geminivirus (MSV) coat polypeptide (CP) promoter, a rice yellow stripe 1 (YS1)-like promoter, a rice yellow stripe 2 (OsYSL2) promoter, and a combination thereof;
iii. comprises a DNA sequence that is at least 95% identical to one or more of SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 70 or SEQ ID NO: 71;
iv. is a rice tungro bacilliform virus (RTBV) promoter; or
v. comprises a DNA sequence that is at least 95% identical to one or more of SEQ ID NO: 65 or SEQ ID NO: 66.
9. A seed of the modified corn plant of claim 1 , wherein the seed comprises the first, second, and third recombinant expression cassettes.
10. The seed of claim 9 , wherein a progeny plant grown from the seed is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not comprise the first, second, and third recombinant expression cassettes.
11. A commodity or commodity product produced from the seed of claim 9 , comprising the first, second, and third recombinant expression cassettes.
12. A method comprising planting the seed of claim 9 in a growth medium or soil.
13. A plurality of modified corn plants in a field, each modified corn plant comprising the modified corn plant of claim 1 .
14. A method for producing a modified corn plant, the method comprising a method selected from the group consisting of (1)-(17):
(1) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, wherein the corn cell comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, and a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second and third recombinant expression cassettes; and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(2) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176, wherein the corn cell comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, and a third recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second, and third recombinant expression cassettes; and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(3) a. introducing into a corn cell a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 and a third recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second, and third recombinant expression cassettes; and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassette;
(4) a. introducing into a corn cell 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, 2) a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, and 3) a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first, second, and third recombinant expression cassettes; and wherein the modified corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(5) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the first modified corn plant relative to a wildtype control, and wherein the second modified corn plant comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 and a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes; wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(6) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, wherein the second modified corn plant comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the second modified corn plant relative to a wildtype control, and wherein the second modified corn plant comprises a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes; wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(7) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176, wherein the second modified corn plant comprises a second recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the second modified corn plant relative to a wildtype control, and wherein the second modified corn plant comprises a third recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168; and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes; wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(8) a. performing one or more crossings using one or more modified corn plants, wherein the one or more modified corn plants are selected from the group consisting of:
i. a modified corn plant comprising a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the modified corn plant relative to a wildtype control,
ii. a modified corn plant comprising a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168,
iii. a modified corn plant comprising a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176,
iv. a modified corn plant comprising a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the modified corn plant relative to a wildtype control, and comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168,
v. a modified corn plant comprising a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, wherein the expression or activity of one or more endogenous GA20 oxidase genes is reduced in the modified corn plant relative to a wildtype control, and comprises a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176, and
vi. a modified corn plant comprising a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 and a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176, and
b. producing a progeny corn plant comprising the first, second, and third recombinant expression cassettes and has the reduced expression of the one or more endogenous GA20 oxidase genes; wherein the progeny corn plant is semi-dwarf and has one or more improved ear traits, relative to a control corn plant that does not have the first, second and third recombinant expression cassettes;
(9) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises (1) one or more mutations and/or edits in one or more endogenous GA20 oxidase genes and (2) a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a second plant-expressible promoter; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes and the one or more mutations and/or edits, and wherein the level of expression or activity of the one or more endogenous GA20 oxidase genes in the modified corn plant is reduced relative to a control plant not having the one or more mutations and/or edits;
(10) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises (1) one or more mutations and/or edits in one or more endogenous GA20 oxidase genes and (2) a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a second plant-expressible promoter; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes and the one or more mutations and/or edits, and wherein the level of expression or activity of the one or more endogenous GA20 oxidase genes in the modified corn plant is reduced relative to a control plant not having the one or more mutations and/or edits;
(11) a. mutating or editing one or more endogenous GA20 oxidase genes in a corn cell, wherein the corn cell comprises (1) a first recombinant expression cassette comprising a encoding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a first plant-expressible promoter and (2) a second recombinant expression cassette comprising a encoding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a second plant-expressible promoter; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes and the one or more mutations and/or edits, and wherein the level of expression or activity of the one or more endogenous GA20 oxidase genes in the modified corn plant is reduced relative to a control plant not having the one or more mutations and/or edits;
(12) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
(13) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176, wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes; wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
(14) a. introducing into a corn cell 1) a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, and 2) a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes; wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
(15) a. crossing a first modified corn plant with a second modified corn plant, wherein the first modified corn plant comprises a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, and wherein the second modified corn plant comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; and
b. producing a progeny corn plant comprising the first and second recombinant expression cassettes; wherein the progeny corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes;
(16) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a second plant-expressible promoter; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes; wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes; and
(17) a. introducing into a corn cell a first recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a first plant-expressible promoter, and wherein the corn cell comprises a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a second plant-expressible promoter; and
b. regenerating or developing a modified corn plant from the corn cell, wherein the modified corn plant comprises the first and second recombinant expression cassettes;
wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes.
15. A modified corn plant comprising 1) one or more mutations or edits at or near one or more endogenous GA20 oxidase genes, wherein the expression or activity of the one or more endogenous GA20 oxidase genes is reduced relative to a wildtype control plant, 2) a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a first plant-expressible promoter, and 3) a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a second plant-expressible promoter.
16. A plurality of modified corn plants in a field, each modified corn plant comprising the modified corn plan of claim 15 .
17. A recombinant DNA construct comprising:
a. 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more GA20 oxidase genes, wherein the non-coding RNA comprises a targeting sequence that is (a) at least 95% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 9, and (b) at least 95% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA20 oxidase protein that has at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 15, and wherein the transcribable DNA sequence is operably linked to a first plant-expressible promoter, 2) a second recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a second plant-expressible promoter, and 3) a third recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a third plant-expressible promoter; or
b. a first recombinant expression cassette comprising a coding sequence for a COL polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168 operably linked to a first plant-expressible promoter, and a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176 operably linked to a second plant-expressible promoter.
18. A modified corn plant or a plant part thereof comprising 1) a first recombinant expression cassette comprising a first DNA sequence encoding a CONSTANS-like (COL) polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 168, and 2) a second recombinant expression cassette comprising a second DNA sequence encoding a MADS-box polypeptide that has at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 176; wherein the modified corn plant has one or more improved ear traits, relative to a control corn plant that does not have the first and second recombinant expression cassettes.
19. A seed of the modified corn plant of claim 18 , wherein the seed comprises the first and second recombinant expression cassettes.
20. A commodity or commodity product produced from claim 19 , comprising the first and second recombinant expression cassettes.
21. A method comprising planting the seed of claim 19 in a growth medium or soil.
22. A plurality of modified corn plants in a field, each modified corn plant comprising the modified corn plant of claim 18 .
23. The modified corn plant of claim 18 , wherein the first DNA sequence encoding the COL polypeptide is operably linked to a first plant-expressible promoter, and the second DNA sequence encoding the MADS-box polypeptide is operably linked to a second plant-expressible promoter.
24. A plurality of modified corn plants in a field, each modified corn plant comprising the corn plant of claim 23 .
25. A transformation vector comprising the recombinant DNA construct of claim 17 .
26. A modified corn plant or a plant part thereof comprising the recombinant DNA construct of claim 17 .