Transcription factor stress-related proteins and methods of use in plants
A transgenic plant transformed by a transcription factor stress-related protein (TFSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated TFSRP, and isolated nucleic acid coding TFSRP, and vectors and host cells containing the latter. Further provided are methods of producing transgenic plants expressing TFSRP, methods of increasing expression of other genes of interest using the TFSRP, methods of identifying novel TFSRP, and methods of modifying the expression of TFSRP in plants.
1. A transgenic plant cell transformed with an isolated polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence as set forth in SEQ ID NO:12; and
b) a polynucleotide encoding a polypeptide having a sequence as set forth in SEQ ID NO:19.
2. The plant cell of claim 1 , wherein the polynucleotide has the sequence as set forth in SEQ ID NO:12.
3. The plant cell of claim 1 , wherein the polynucleotide encodes the polypeptide having the sequence as set forth in SEQ ID NO:19.
4. A transgenic plant transformed with an isolated polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence as set forth in SEQ ID NO:12; and
b) a polynucleotide encoding a polypeptide having a sequence as set forth in SEQ ID NO:19.
5. The plant of claim 4 , wherein the polynucleotide has the sequence as set forth in SEQ ID NO:12.
6. The plant of claim 4 , wherein polynucleotide encodes the polypeptide having the sequence as set forth in SEQ ID NO:19.
7. The plant of claim 4 , wherein the plant is a monocot.
8. The plant of claim 4 , wherein the plant is a dicot.
9. The plant of claim 4 , wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grasses, and a forage crop plant.
10. A seed which is true breeding for a transgene comprising a polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence as set forth in SEQ ID NO:12; and
b) a polynucleotide encoding a polypeptide having a sequence as set forth in SEQ ID NO:19.
11. The seed of claim 10 , wherein the polynucleotide has the sequence as set forth in SEQ ID NO:12.
12. The seed of claim 10 , wherein the polynucleotide encodes the polypeptide having the sequence as set forth in SEQ ID NO:19.
13. An isolated nucleic acid comprising a polynucleotide selected from the group consisting of:
a) a polynucleotide having a sequence as set forth in SEQ ID NO:12; and
b) a polynucleotide encoding a polypeptide having a sequence as set forth in SEQ ID NO:19.
14. The isolated nucleic acid of claim 13 , wherein the polynucleotide has the sequence as set forth in SEQ ID NO:12.
15. The isolated nucleic acid of claim 13 , wherein the polynucleotide encodes the polypeptide having the sequence as set forth in SEQ ID NO:19.
16. A method of producing a drought-tolerant transgenic plant, the method comprising the steps of:
a) transforming a plant cell with an expression vector comprising a polynucleotide encoding a polypeptide having a sequence as set forth in SEQ ID NO:19;
b) growing the transformed plant cell to generate transgenic plants; and
c) screening the transgenic plants generated in step b) to identify a transgenic plant that expresses the polypeptide and exhibits increased tolerance to drought stress as compared to a wild type variety of the plant.
17. The method of claim 16 , wherein the polynucleotide has a sequence as set forth in SEQ ID NO:12.