Granted Patent B2

US 12,369,822 · App. 17/643,029 · Granted Jul 29, 2025

Inventors: Brendan McCarthy (Encinitas, CA); Huashi Zhang (San Juan Capistrano, CA); Robert James Boock (Carlsbad, CA); Michael Christophe Walsh (Seattle, WA)

Assignee: Allez Health Inc.

A61B5/14865A61B5/14532A61M2230/201

Abstract

Claims (24)

1. A method of manufacturing a metabolic sensor, the method comprising:

assembling a working wire for the metabolic sensor, the assembling comprising:

forming an interference layer on a substrate, the substrate having an electrically conductive surface;

forming an enzyme layer on the interference layer; and

forming a glucose limiting layer on the enzyme layer; and

exposing the interference layer to a gas, the gas comprising an oxidizing agent, wherein the exposing is performed prior to sterilizing the working wire.

2. The method of claim 1 , wherein exposing the interference layer to the gas is performed prior to forming the enzyme layer.

3. The method of claim 1 , wherein exposing the interference layer to the gas is performed after forming the enzyme layer, wherein the exposing further comprises exposing the enzyme layer to the gas.

4. The method of claim 1 , wherein the oxidizing agent is ethylene oxide (EtO), and the gas has an EtO concentration greater than or equal to 2500 ppm.

5. The method of claim 1 , wherein the oxidizing agent is selected from propylene oxide (CH 3 CHCH 2 O), epichlorohydrin (C 3 H 5 CIO), hydrogen peroxide (H 2 O 2 ), peracetic acid (C 2 H 4 O 3 ), or a Brønsted acid.

6. The method of claim 5 , wherein the oxidizing agent is H 2 O 2 having a concentration of greater than or equal to 1200 ppm in the gas.

7. The method of claim 1 , further comprising:

assembling the metabolic sensor comprising the working wire;

coupling the metabolic sensor to an electronic operating circuitry;

placing the electronic operating circuitry in a non-sterile container with the metabolic sensor; and

sterilizing the non-sterile container having the metabolic sensor and the electronic operating circuitry, wherein the sterilizing comprises a gas sterilization.

8. The method of claim 1 , wherein forming the interference layer comprises:

mixing a monomer with a solvent to form a monomer solution, the monomer comprising pyrrole, phenylenediamine (PDA), aminophenol, aniline, or combinations thereof;

applying the monomer solution to the substrate; and

electropolymerizing the monomer solution to form a polymer on the substrate.

9. The method of claim 1 , wherein the enzyme layer comprises:

enzymes;

an immobilization matrix; and

a polymeric crosslinking agent crosslinking the enzymes and the immobilization matrix, creating an enzyme immobilization network.

Assignments (2)

Continuity (4)

References Cited (119)