Tissue-penetrating electrochemical sensor featuring a co-electrodeposited thin film comprised of polymer and bio-recognition element
A method and device to impart the ability to selectively quantify chemical/biochemical analytes occupying physiological fluids via an automated process that allow the precise and spatially-defined simultaneous deposition of a thin-film of polymer containing an immobilized biorecognition element dispersed therein. A tissue penetrating electrochemical sensor comprises at least one working electrode and at least one of a reference electrode and a counter electrode.
1. A tissue-penetrating electrochemical sensor device for the quantification of a chemical or biochemical entity in a physiological fluid, the device comprising:
an array of microelectrodes, the array of microelectrodes comprising a working electrode, a reference electrode, and a counter electrode; and
a polymer film comprising an electroactive monomer precursor and a selective recognition element, the polymer film electro-deposited on at least a portion of the working electrode in response to immersion of the working electrode, the reference electrode, and the counter electrode in a solution and application of a potential applied between the working electrode and at least one of the reference electrode and the counter electrode, the solution comprising
the selective recognition element, wherein the electroactive monomer precursor is dissolved in the solution, wherein application of the potential occurs during the immersion of the working electrode, the reference electrode, and the counter electrode.
2. The device of claim 1 , wherein the electroactive monomer precursor is selected for at least one of its perm-selective properties following conversion to a thin-film of polymer, its charge rejection properties following conversion to a thin-film of polymer, its anti-biofouling properties following conversion to a thin-film of polymer, its porosity following conversion to a thin-film of polymer, its diffusion-limiting nature following conversion to a thin-film of polymer, and its self-limited growth during conversion to a thin-film of polymer.
3. The device of claim 1 , wherein the selective recognition element comprises at least one of an enzyme, a biocatalyst, an inorganic catalyst, an ion-selective material, an antibody, aptamer, an oligonucleotide, an electrochemical redox mediator, a cell, and an organelle.
4. The device of claim 1 , wherein the potential is at least one of a fixed potential and a time-varying potential.
5. The device of claim 4 , wherein the potential is applied using one of an amperometric technique, a voltammetric technique, a conductometric technique, or a coulometric technique.
6. The device of claim 4 , wherein the potential is selected to result in a thickness of the polymer film ranging from about 5 to about 5000 nanometers.
7. The device of claim 4 , wherein the potential is applied for a specified time duration to result in a thickness of the polymer film ranging from about 5 to about 5000 nanometers.
8. The device of claim 4 , wherein the potential is selected to pass a specified amount of charge through the working electrode to result in a thickness of the polymer film ranging from about 5 to about 5000 nanometers.
9. A method for the fabrication of a tissue-penetrating electrochemical sensor for the quantification of a chemical or biochemical entity in a physiological fluid, the method comprising:
immersing an array of microelectrodes in a solution comprising a selective recognition element and an electroactive monomer precursor dissolved in the solution, the array of microelectrodes comprising a working electrode, a reference electrode, and a counter electrode;
applying, during the immersion of the working electrode, the reference electrode, and the counter electrode in the solution, a potential between the working electrode and at least one of the reference electrode and the counter electrode, the application of the potential resulting in electro-deposition on at least a portion of the working electrode of a polymer film comprising the electroactive monomer precursor and the selective recognition element.
10. The method of claim 9 , wherein the electroactive monomer precursor is selected for at least one of its perm-selective properties following conversion to a thin-film of polymer, its charge rejection properties following conversion to a thin-film of polymer, its anti-biofouling properties following conversion to a thin-film of polymer, its porosity following conversion to a thin-film of polymer, its diffusion-limiting nature following conversion to a thin-film of polymer, and its self-limited growth during conversion to a thin-film of polymer.
11. The method of claim 9 , wherein the selective recognition element comprises at least one of an enzyme, a biocatalyst, an inorganic catalyst, an ion-selective material, an antibody, aptamer, an oligonucleotide, an electrochemical redox mediator, a cell, and an organelle.
12. The method of claim 9 , wherein the potential is at least one of a fixed potential and a time-varying potential.
13. The method of claim 12 wherein the potential is applied using one of an amperometric technique, a voltammetric technique, a conductometric technique, or a coulometric technique.
14. The method of claim 12 , wherein the potential is selected to result in a thickness of the polymer film ranging from about 5 to about 5000 nanometers.
15. The method of claim 12 , wherein to potential is applied for a specified time duration to result in a thickness of the polymer film ranging from about 5 to about 5000 nanometers.
16. A method for the fabrication of a tissue-penetrating electrochemical sensor for the quantification of a chemical or biochemical entity in a physiological fluid, the method comprising:
immersing an array of microelectrodes in a solution comprising a selective recognition element and an electroactive monomer precursor dissolved in the solution, the array of microelectrodes comprising a working electrode positioned on a first tissue-penetrating electrochemical sensor and at least one additional electrode positioned on a second tissue-penetrating electrochemical sensor;
applying a voltage ranging from −0.5 volt to +1.5 volt between the working electrode and the at least one additional electrode such that current flows through the working electrode; and
electrodepositing on the working electrode a polymer film from the electroactive monomer precursor and the selective recognition element, the polymer film having a thickness ranging from about 5 to about 5000 nanometers.
17. The device of claim 1 , wherein the selective recognition element and the electroactive monomer precursor are dispersed uniformly and physically entrapped in the polymer film.
18. The method of claim 9 , wherein the selective recognition element and the electroactive monomer precursor are dispersed uniformly and physically entrapped in the polymer film.
19. The method of claim 16 , wherein the selective recognition element and the electroactive monomer precursor are dispersed uniformly and physically entrapped in the polymer film.