Formulation containing TLR agonist and methods of use
Stable aqueous formulations of adjuvant comprising a TLR7/8 agonist or a TLR4 agonist with a helper lipid that are adsorbed to alum are provided. Compositions and methods of using the formulations for stimulating an immune response are also provided.
1. A composition comprising:
(a) a TLR4 agonist, wherein the TLR4 agonist is GLA;
(b) a helper lipid; and
(c) an aluminum salt,
wherein the helper lipid is DPTAP and the aluminum salt is aluminum phosphate, or
wherein the helper lipid is DPPC and the aluminum salt is aluminum hydroxide,
wherein the composition is an aqueous formulation comprising a stable nanosuspension having a particle size of 400 nm or less, the TLR4 agonist is adsorbed to the aluminum salt at 25 percent or more of the aluminum salt, and the TLR4 agonist and the helper lipid are present in a molar ratio of about 1:2.
2. The composition of claim 1 , wherein the stable nanosuspension has a particle size of 200 nm or less.
3. The composition of claim 1 , wherein the TLR4 agonist comprises a synthetic GLA of Formula (IV):
or a pharmaceutically acceptable salt thereof, wherein:
L 1 , L 2 , L 3 , L 4 , L 5 , and L 6 are the same or different and independently —O—, —NH—or (CH 2 )-;
L 7 , L 8 , L 9 , and L 10 are the same or different and independently absent or ≠C (=O)—;
Y 1 is an acid functional group;
Y 2 and Y 3 are the same or different and independently —OH, —SH, or an acid functional group;
Y 4 is —OH or —SH;
R 1 , R 3 , R 5 , and R 6 are the same or different and independently C 8-13 alkyl; and
R 2 and R 4 are the same or different and independently C 6-11 alkyl.
4. The composition of claim 3 , wherein the TLR4 agonist comprises a synthetic GLA of Formula (V):
or a pharmaceutically acceptable salt thereof, wherein
R 1 , R 3 , R 5 , and R 6 are C 11 -C 20 alkyl; and
R 2 and R 4 are C 12 -C 20 alkyl
5. The composition of claim 4 , wherein the TLR4 agonist comprises a synthetic GLA of formula:
or a pharmaceutically acceptable salt thereof.
6. The composition of claim 1 , further comprising an antigen.
7. The composition of claim 6 , wherein the antigen is selected from a tuberculosis-related antigen, influenza-related antigen, hemagglutinin-related antigen, cancer-related antigen, viral-related antigen, and amebiasis-related antigen.
8. The composition of claim 7 , wherein:
the tuberculosis-related antigen is selected from the group consisting of ID93, ID91, and BCG;
the influenza-related antigen is selected from the group consisting of HSNI, influenza A, influenza B, and influenza C;
the amebiasis-related antigen is LecA; or
the viral-related antigen is selected from the group consisting of hepatitis B and hepatitis C.
9. The composition of claim 1 , wherein the composition is stable at 2-8° C. for at least 16 weeks.
10. The composition of claim 1 , wherein the composition is a vaccine.