IP Library › Granted Patent US 12,650,427
Granted Patent B2
US 12,650,427 · App. 17/795,991 · Granted Jun 9, 2026

Analyte detection with redox active polymer-coated electrode

Inventors: Jason John Davis (Oxford, GB); Paulo Roberto Bueno (São Paulo, BR)
Assignees: Oxford University Innovation Limited; Universidade Estadual Paulista “Júlio De Mesquita Filho”—UNESP
G01N33/5438G01N27/227G01N33/6872G01N2333/4737
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Quick Facts
Patent No.
US 12,650,427
App. No.
17/795,991
Granted
Jun 9, 2026
Kind
B2
Abstract

The present invention relates to the electrochemical detection of an analyte by capacitance spectroscopy using a redox active polymer-coated electrode such as a polyaniline-coated electrode.

Claims (30)

1 . An electrochemical method of sensing a target species, which method comprises:

(A) contacting a carrier medium that may comprise said target species with an electrode that comprises:

(i) an electrically conductive substrate;

(ii) a film comprising a redox-active, electrically conductive polymer disposed on the substrate, wherein the redox-active, electrically conductive polymer is polyaniline (PANI) and wherein the film is doped with phytic acid; and

iii) at least one receptor associated with the film, wherein the receptor is capable of binding to the target species; and

(B) determining, via electrochemical capacitance spectroscopy, whether the target species is present in the carrier medium.

2 . The method of claim 1 , wherein:

(i) the redox-active, electrically conductive polymer is PANI and comprises a plurality of redox active monomeric repeating units which are aniline units; and

(ii) a surface coverage of said plurality of redox active monomeric repeating units on the electrically conductive substrate is from 3 to 40 nmolcm −2 , as measured from a redox capacitance, Cr, of the electrode obtained via circuit fitting and in the presence of a control carrier medium containing no target species.

3 . The method of claim 1 , wherein the receptor is selected from the group consisting of aptamers, antibodies, antibody fragments, oligosaccharides, peptides and proteins.

4 . The method of claim 1 , wherein the receptor is an aptamer.

5 . The method of claim 1 , wherein the electrically conductive substrate is a gold substrate.

6 . The method of claim 1 , wherein the electrically conductive substrate is not a gold substrate.

7 . The method of claim 1 , wherein the electrically conductive substrate comprises carbon, platinum, silver, ruthenium oxide or indium tin oxide (ITO).

8 . The method of claim 1 , wherein the electrically conductive substrate is a screen-printed electrode (SPE) substrate.

9 . The method of claim 1 , wherein the target species is an antigen or analyte selected from the group consisting of dengue NS1 protein; angiotensin I converting enzyme (peptidyl-dipeptidase A); adiponectin; advanced glycosylation end product-specific receptor; alpha-2-HS-glycoprotein; angiogenin, ribonuclease, RNase A family, 5; apolipoprotein A-1; apolipoprotein B (including Ag (x) antigen); apolipoprotein E; BCL2-associated X protein; B-cell CLL/lymphoma 2; complement C3; chemokine (C-C motif) ligand 2; CD 14, soluble; CD 40, soluble; cdk5; pentraxin-related; cathepsin B; dipeptidyl peptidase IV; Epidermal growth factor; endoglin; Fas; fibrinogen; ferritin; growth hormone 1; alanine aminotransferase; hepatocyte growth factor; haptoglobin; heat shock 70 kDa protein 1 B; intercellular adhesion molecule 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1 receptor; insulin-like growth factor binding protein 1; insulin-like growth factor binding protein 2; insulin-like growth factor-binding protein 3; interleukin 18; interleukin 2 receptor, alpha; interleukin 2 receptor, beta; interleukin 6 (interferon, beta 2); interleukin 6 receptor; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin 8; activin A; leptin (obesity homolog, mouse); plasminogen activator, tissue; proopiomelanocortin (adrenocorticotropin/beta-lipotropin/alpha-melanocyte stimulating hormone/beta-melanocyte stimulating hormone/beta-endorphin); proinsulin; resistin; selectin e (endothelial adhesion molecule 1); selectin P (granule membrane protein 140 kDa, antigen CD62); serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serum/glucocorticoid regulated kinase; sex hormone-binding globulin; transforming growth factor, beta 1 (Camurati-Engelmann disease); TIMP metallopeptidase inhibitor 2; tumor necrosis factor receptor superfamily, member 1 B; vascular cell adhesion molecule 1 (VCAM-1); vascular endothelial growth factor; Factor II, Factor V, Factor VIII, Factor IX, Factor XI, Factor XII, F/fibrin degradation products, thrombin-antithrombin III complex, fibrinogen, plasminogen, prothrombin, and von Willebrand factor and the like, Markers useful for diabetes include for example C-reactive protein; glucose; insulin; TRIG; GPT; HSPA1 B; IGFBP2; LEP; ADIPOQ; CCL2; ENG; HP; IL2RA; SCp; SHBG; and TIMP2.

10 . The method of claim 1 , wherein step (B) comprises determining a concentration of the target species.

11 . The method of claim 1 , wherein the electrochemical capacitance spectroscopy comprises determining a conductance of the electrode.

12 . The method of claim 11 , wherein said determining, via electrochemical capacitance spectroscopy, comprises:

(B)(i) obtaining a measurement of the conductance of the electrode when in contact with the carrier medium that may comprise said target species; and

(B)(ii) comparing said measurement with the conductance of the electrode when in contact with a reference carrier medium that does not comprise said target species.

13 . The method of claim 1 , wherein said determining, via electrochemical capacitance spectroscopy, comprises:

(B)(i) obtaining a measurement of a redox capacitance of the electrode when in contact with the carrier medium that may comprise said target species; and

(B)(ii) comparing said measurement with the redox capacitance of the electrode when in contact with a reference carrier medium that does not comprise said target species.

14 . An electrochemical method of sensing a target species, which method comprises:

(A) contacting a carrier medium that may comprise said target species with an electrode that comprises:

(i) an electrically conductive substrate;

(ii) a film comprising a redox-active, electrically conductive polymer disposed on the substrate, wherein the redox-active, electrically conductive polymer is polyaniline (PANI) and wherein the film is doped with phytic acid; and

(iii) at least one receptor associated with the film, wherein the receptor is capable of binding to the target species; and

(B) determining, via electrochemically determining a conductance of the electrode, whether the target species is present in the carrier medium.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 11, 2022
From: JASON JOHN DAVIS
To: OXFORD UNIVERSITY INNOVATION LIMITED
Reel/Frame 061374/0283 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 11, 2022
From: BUENO, PAULO ROBERTO
To: UNIVERSIDADE ESTADUAL PAULISTA "JULIO DE MESQUITA FILHO" - UNESP
Reel/Frame 061374/0327 →
Priority Claims (2)
GB 2001239 · Jan 29, 2020 · national
GB 2016287 · Oct 14, 2020 · national
Continuity (1)
Related Publication 20230081940A1 · Mar 16, 2023
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