Composition comprising of a conducting polymer for detecting, capturing, releasing, and collecting cell
The present invention relates to a composition in which a conducting polymer is doped with a dopant, and a diagnostic apparatus, and more particularly, to a composition which is used to diagnose a disease and detect a biomaterial and also used for qualification and diagnosis by effectively and non-destructively collecting a captured biomaterial. Further, the composition can maximize capturing efficiency by being attached to a surface of a nano-structured scaffold and can be used as an ultrahigh-sensitive sensor using various linked bodies.
1. A method for detecting and collecting a Circulating Tumor Cell (CTC) in a sample, comprising:
contacting the sample with a doped composition comprising a dopant doped within a conducting polymer to detect the CTC in the sample, and
applying a negative electric field to the doped composition to naturally separate the dopant with the CTC,
wherein the dopant is doped within the conducting polymer during electropolymerization to form the doped composition and naturally separates from the polymer when the negative electric field is applied;
the doped composition comprises an antibody bonded to the conducting polymer via a linker comprising a single or multiple linking body/bodies, the linking body comprising biotin and streptavidin, and the doped composition is not a porous film;
the conducting polymer is polypyrrole,
the doped composition has a nanowire structure, and
the dopant is connected to the linker to control the release of the antibody with CTC.
2. The method for detecting and collecting a CTC of claim 1 , wherein the antibody is an anti-EpCAM (Epithelial Cell Adhesion Molecule), an anti-PSA (Prostate-Specific Antigen: KLK3 (Kallikrein)), an anti-PSMA (Prostate-Specific Membrane Antigen), an anti-PSCA (Prostate Stem Cell Antigen), an anti-STEAP (Six Transmembrane Epithelial Antigen of the Prostate), an anti-hTERT (human TElomerase Reverse Transcriptase), an anti-WT1 (Wilms tumor 1), an anti-MAGE (Melanoma Antigen Family A)-A2, an anti-5T4(oncofetal antigen 5T4), an anti-MAGE-A3, an anti-MUC1 (Mucin 1, cell surface associated), an anti-Her-2/neu (human epidermal growth factor receptor 2), an anti-CEA (Carcinoembryonic Antigen), an anti-survivin, an anti-MAGE-C1, or an anti-MAGE-C2.
3. The method for detecting and collecting a CTC of claim 1 , wherein the linker comprises more than 1 to 50 linking bodies comprised of biotin and streptavidin.
4. The method for detecting and collecting a CTC of claim 1 , wherein the composition is attached onto a surface of gold (Au), platinum (Pt), silver (Ag), copper (Cu), iron (Fe), or ITO (Indium Tin Oxide).
5. The method for detecting and collecting a CTC of claim 1 , further comprising measuring a change in an electric current.