Granted Patent B2

US 12,529,096 · App. 17/816,984 · Granted Jan 20, 2026

Inventor: Toon Verheyen (Solna, SE)

Assignee: 10X GENOMICS, INC.

C12Q1/6853C12Q1/6806C12Q1/6841

Abstract

Claims (26)

1 . A method, comprising:

generating a rolling circle amplification (RCA) product by performing a rolling circle amplification (RCA) reaction on a template nucleic acid molecule, wherein the RCA product comprises a biotin moiety conjugated to one or more nucleotides of the RCA product; and

adding unconjugated avidin or unconjugated streptavidin to the RCA product, thereby generating a compacted RCA product.

2 . The method of claim 1 , wherein the RCA reaction comprises incorporating non-biotinylated nucleotides and one or more biotinylated nucleotides into the RCA product to generate the RCA product comprising the biotin moiety.

3 . The method of claim 2 , wherein the one or more biotinylated nucleotides comprise a biotinylated dATP, a biotinylated dCTP, a biotinylated dGTP, and/or a biotinylated dUTP.

4 . The method of claim 2 , wherein the ratio of the non-biotinylated nucleotides to the biotinylated nucleotide(s) is between 100:1 and 10,000:1.

5 . The method of claim 2 , wherein the ratio of the non-biotinylated nucleotides to the biotinylated nucleotide(s) is 4000:1.

6 . The method of claim 2 , wherein the biotin moiety conjugated to one or more nucleotides comprises a linker of 7 to 14 atoms.

7 . The method of claim 2 , wherein the unconjugated avidin or the unconjugated streptavidin is added to the RCA product in the presence of MgCl 2 at a concentration of between 50 mM to 1 M.

8 . The method of claim 1 , wherein the template nucleic acid molecule is a circular molecule or circularized molecule.

9 . The method of claim 8 , wherein the template nucleic acid molecule is a circularized molecule and the circularized molecule is a circularized probe generated from a circularizable probe or probe set.

10 . The method of claim 9 , wherein the circularized probe is a circularized padlock probe complementary to a target nucleic acid molecule, and wherein the method further comprises hybridizing said padlock probe to said target nucleic acid molecule and circularizing said padlock probe to generate said circularized probe.

11 . The method of claim 10 , wherein the target nucleic acid molecule is an RNA molecule.

12 . The method of claim 11 , wherein the RNA molecule is an mRNA molecule.

13 . The method of claim 10 , wherein the target nucleic acid molecule is a complementary DNA (cDNA) molecule.

14 . The method of claim 10 , wherein the target nucleic acid molecule is in a biological sample comprising a plurality of cells.

15 . The method of claim 14 , wherein the biological sample is or is derived from a fresh, fresh frozen or formalin-fixed paraffin embedded (FFPE) tissue.

16 . The method of claim 1 , comprising detecting the compacted RCA product.

17 . The method of claim 1 , wherein generating the compacted RCA product reduces the size of the RCA product by about 20%.

18 . A method for analysis, comprising:

(a) contacting a biological sample with a probe to provide the probe bound to a target nucleic acid molecule;

(b) generating a rolling circle amplification (RCA) product in said biological sample by performing a rolling circle amplification (RCA) reaction using said probe as a template, wherein the RCA product comprises a biotin moiety conjugated to one or more nucleotides of the RCA product;

(c) using unconjugated avidin or unconjugated streptavidin to generate a compacted RCA product; and

(d) detecting the compacted RCA product.

19 . The method of claim 18 , wherein the probe is a circular probe.

20 . The method of claim 18 , wherein the probe is a circularizable probe, wherein the method further comprises, prior to the generating in step (b), circularizing the circularizable probe.

Assignments (2)

Continuity (2)

References Cited (400)