IP Library Granted Patent US 11,680,292
Granted Patent B2
US 11,680,292 · App. 17/840,569 · Granted Jun 20, 2023

Flow cells

Inventors: Jeffrey S. Fisher (San Diego, CA); Brian D. Mather (San Diego, CA); Maria Candelaria Rogert Bacigalupo (Encinitas, CA); Justin Fullerton (San Diego, CA); Ludovic Vincent (San Diego, CA); Lewis J. Kraft (San Diego, CA); Sahngki Hong (San Diego, CA); Boyan Boyanov (San Diego, CA); M. Shane Bowen (Encinitas, CA); Sang Park (San Diego, CA); Wayne N. George (Ilford, GB); Andrew A. Brown (Cambridge, GB)
Assignees: Illumina, Inc.; Illumina Cambridge Limited
C12Q1/6869C08F220/56C08G77/04G03F7/0757G03F7/165G03F7/0002G03F7/0046
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Quick Facts
Patent No.
US 11,680,292
App. No.
17/840,569
Granted
Jun 20, 2023
Kind
B2
Abstract

An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

Claims (51)

1. A flow cell, comprising:

a support;

a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;

a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and

a primer attached to the block-specific functional group of at least one of the blocks;

wherein the patterned resin is a polyhedral oligomeric silsesquioxane resin (POSS)-based resin, and wherein the POSS-based resin is a cross-linked epoxy POSS resin.

2. A flow cell, comprising:

a support;

a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;

a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and

a primer attached to the block-specific functional group of at least one of the blocks;

wherein the patterned resin is selected from the group consisting of a polyhedral oligomeric silsesquioxane resin (POSS)-based resin, an epoxy resin, a poly(ethylene glycol) resin, a polyether resin, an acrylic resin, an acrylate resin, a methacrylate resin, and combinations thereof;

and wherein the block copolymer includes:

a first block including an acrylamide monomer having an amino group as its block-specific functional group; and

a second block including an azido acetamido pentyl acrylamide monomer having an azido group as its block-specific functional group.

3. The flow cell as defined in claim 2 , wherein the block copolymer is:

wherein R is hydrogen or a polymer initiating species end group, n ranges from 1 to 10,000, and m ranges from 1 to 10,000.

4. A flow cell, comprising:

a support;

a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;

a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and

a primer attached to the block-specific functional group of at least one of the blocks;

wherein the patterned resin is selected from the group consisting of a polyhedral oligomeric silsesquioxane resin (POSS)-based resin, an epoxy resin, a poly(ethylene glycol) resin, a polyether resin, an acrylic resin, an acrylate resin, a methacrylate resin, and combinations thereof;

and wherein the block copolymer is:

wherein n ranges from 1 to 10,000, and m ranges from 1 to 10,000.

5. A flow cell, comprising:

a support;

a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;

a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and

a primer attached to the block-specific functional group of at least one of the blocks;

wherein:

the patterned resin is an amorphous fluoropolymer, and wherein the block copolymer includes:

a first block including a monomer having a trifluoromethyl group as its block-specific functional group; and

a second block including a monomer having a primer-grafting functional group as its block-specific functional group; or

the block copolymer includes:

a first block including a monomer having a primer-grafting functional group as its block-specific functional group; and

a second block including a monomer to adjust an interaction parameter to drive phase separation of the first and second blocks, wherein:

the primer-grafting functional group is an azido group; and

the block-specific functional group of the monomer of the second block is selected from the group consisting of an amino group, an alcohol group, an aryl group, and a charged group; or

the block copolymer is a terpolymer including a first block, a second block, and a third block, the block-specific functional group of the first block is attached to the patterned resin, the block-specific functional group of the second block is attached to the primer, and the block-specific functional group of the third block is attached to an other primer that is different than the primer, or to an enzyme; or

the block copolymer is a terpolymer including a first block, a second block, and a third block, the block-specific functional group of the first block is attached to the patterned resin, the block-specific functional group of the second block is attached to the primer, and the block-specific functional group of the third block affects a surface free energy of the block copolymer or affects stability of the block copolymer; or

the depressions are selected from the group consisting of wells and trenches; or

the patterned resin and the block copolymer each have a surface free energy within a range of from about 25 mN/m to about 50 mN/m.

6. A flow cell, comprising:

a support;

a patterned polyhedral oligomeric silsesquioxane (POSS)-based resin on the support, the patterned POSS-based resin including depressions separated by interstitial regions;

a segregated block copolymer on the patterned POSS-based resin in the depressions, wherein one block of the segregated block copolymer has a functional group attached to the patterned POSS-based resin and an other block of the segregated block copolymer has an other functional group; and

a primer attached to the other functional group.

7. The flow cell as defined in claim 6 , wherein the segregated block copolymer is selected from the group consisting of:

wherein n ranges from 1 to 10,000, and m ranges from 1 to 10,000; and

wherein R is hydrogen or a polymer initiating species end group, n ranges from 1 to 10,000, and m ranges from 1 to 10,000.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 12, 2022
From: GEORGE, WAYNE N.; BROWN, ANDREW A.
To: ILLUMINA CAMBRIDGE LIMITED
Reel/Frame 061064/0573 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 12, 2022
From: FISHER, JEFFREY S.; MATHER, BRIAN D.; ROGERT BACIGALUPO, MARIA CANDELARIA; FULLERTON, JUSTIN; VINCENT, LUDOVIC; KRAFT, LEWIS J.; HONG, SAHNGKI; BOYANOV, BOYAN; BOWEN, M. SHANE; PARK, SANG; YUAN, DAJUN
To: ILLUMINA, INC.
Reel/Frame 061418/0903 →
Continuity (5)
Continuation 17139834 · Dec 31, 2020
Division 16626452
Provisional Application 62743373 · Oct 9, 2018
Provisional Application 62692511 · Jun 29, 2018
Related Publication 20230002822A1 · Jan 5, 2023