Flow cells
An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
1. A flow cell, comprising:
a support;
a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;
a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and
a primer attached to the block-specific functional group of at least one of the blocks;
wherein the patterned resin is a polyhedral oligomeric silsesquioxane resin (POSS)-based resin, and wherein the POSS-based resin is a cross-linked epoxy POSS resin.
2. A flow cell, comprising:
a support;
a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;
a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and
a primer attached to the block-specific functional group of at least one of the blocks;
wherein the patterned resin is selected from the group consisting of a polyhedral oligomeric silsesquioxane resin (POSS)-based resin, an epoxy resin, a poly(ethylene glycol) resin, a polyether resin, an acrylic resin, an acrylate resin, a methacrylate resin, and combinations thereof;
and wherein the block copolymer includes:
a first block including an acrylamide monomer having an amino group as its block-specific functional group; and
a second block including an azido acetamido pentyl acrylamide monomer having an azido group as its block-specific functional group.
3. The flow cell as defined in claim 2 , wherein the block copolymer is:
wherein R is hydrogen or a polymer initiating species end group, n ranges from 1 to 10,000, and m ranges from 1 to 10,000.
4. A flow cell, comprising:
a support;
a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;
a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and
a primer attached to the block-specific functional group of at least one of the blocks;
wherein the patterned resin is selected from the group consisting of a polyhedral oligomeric silsesquioxane resin (POSS)-based resin, an epoxy resin, a poly(ethylene glycol) resin, a polyether resin, an acrylic resin, an acrylate resin, a methacrylate resin, and combinations thereof;
and wherein the block copolymer is:
wherein n ranges from 1 to 10,000, and m ranges from 1 to 10,000.
5. A flow cell, comprising:
a support;
a patterned resin on the support, the patterned resin including depressions separated by interstitial regions;
a block copolymer on the patterned resin in the depressions, each block of the block copolymer having a block-specific functional group that is different from the block-specific functional group of each other block of the block copolymer; and
a primer attached to the block-specific functional group of at least one of the blocks;
wherein:
the patterned resin is an amorphous fluoropolymer, and wherein the block copolymer includes:
a first block including a monomer having a trifluoromethyl group as its block-specific functional group; and
a second block including a monomer having a primer-grafting functional group as its block-specific functional group; or
the block copolymer includes:
a first block including a monomer having a primer-grafting functional group as its block-specific functional group; and
a second block including a monomer to adjust an interaction parameter to drive phase separation of the first and second blocks, wherein:
the primer-grafting functional group is an azido group; and
the block-specific functional group of the monomer of the second block is selected from the group consisting of an amino group, an alcohol group, an aryl group, and a charged group; or
the block copolymer is a terpolymer including a first block, a second block, and a third block, the block-specific functional group of the first block is attached to the patterned resin, the block-specific functional group of the second block is attached to the primer, and the block-specific functional group of the third block is attached to an other primer that is different than the primer, or to an enzyme; or
the block copolymer is a terpolymer including a first block, a second block, and a third block, the block-specific functional group of the first block is attached to the patterned resin, the block-specific functional group of the second block is attached to the primer, and the block-specific functional group of the third block affects a surface free energy of the block copolymer or affects stability of the block copolymer; or
the depressions are selected from the group consisting of wells and trenches; or
the patterned resin and the block copolymer each have a surface free energy within a range of from about 25 mN/m to about 50 mN/m.
6. A flow cell, comprising:
a support;
a patterned polyhedral oligomeric silsesquioxane (POSS)-based resin on the support, the patterned POSS-based resin including depressions separated by interstitial regions;
a segregated block copolymer on the patterned POSS-based resin in the depressions, wherein one block of the segregated block copolymer has a functional group attached to the patterned POSS-based resin and an other block of the segregated block copolymer has an other functional group; and
a primer attached to the other functional group.
7. The flow cell as defined in claim 6 , wherein the segregated block copolymer is selected from the group consisting of:
wherein n ranges from 1 to 10,000, and m ranges from 1 to 10,000; and
wherein R is hydrogen or a polymer initiating species end group, n ranges from 1 to 10,000, and m ranges from 1 to 10,000.