IP Library Granted Patent US 12,330,195
Granted Patent B2
US 12,330,195 · App. 17/856,402 · Granted Jun 17, 2025

In-situ mycoremediation system and process

Inventors: John Gregg (Signal Hill, CA); Laura Gregg (Signal Hill, CA); James Gregg (Signal Hill, CA)
Assignee: Seas Geosciences, LLC
B09C1/105C02F3/347B09C2101/00C02F2103/06
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Quick Facts
Patent No.
US 12,330,195
App. No.
17/856,402
Granted
Jun 17, 2025
Kind
B2
Abstract

An in-situ mycoremediation system and process is provided, including a device with a rod casing having a top end, a bottom end, and a sidewall with one or more perforations, the sidewall defining an internal channel that extends from an intake opening on the top end to the one or more perforations, a sleeve that extends around at least part of the rod casing and that is slidable between at least a first position that covers the one or more perforations and a second position that at least partly uncovers the one or more perforations, and a plumbing line linked to the intake opening and configured to facilitate forcible injection of one more fungal mixtures and/or air via the one or more perforations when the sleeve is in the second position.

Claims (36)

1. A process for in-situ mycoremediation, the process comprising:

driving one or more rod casings into a subsurface zone, the one or more rod casings including one or more orifices that are covered;

partially retracting the one or more rod casings to expose the one or more orifices;

forcibly injecting a fluid via the one or more orifices to initiate fracturing in the subsurface zone;

forcibly injecting a fungal and nutrient mixture via the one or more orifices into the subsurface zone; and

ventilating the subsurface zone with air via the one or more orifices to support a hyphal network of mycelium in the subsurface zone.

2. The process of claim 1 , wherein the fungal and nutrient mixture comprises at least one fungal spore and one or more sugars.

3. The process of claim 1 , further including forcibly injecting air via the one or more orifices.

4. The process of claim 1 , wherein the forcibly injecting a fluid via the one or more orifices to initiate fracturing in the subsurface zone comprises:

forcibly injecting a carbon solution via the one or more orifices to initiate fracturing in the subsurface zone.

5. The process of claim 1 , wherein the forcibly injecting a fluid via the one or more orifices to initiate fracturing in the subsurface zone comprises:

forcibly injecting water via the one or more orifices to initiate fracturing in the subsurface zone.

6. The process of claim 1 , wherein the forcibly injecting a fluid via the one or more orifices to initiate fracturing in the subsurface zone comprises:

forcibly injecting a fluid via the one or more orifices above an overburden pressure of the subsurface zone to initiate fracturing in the subsurface zone.

7. The process of claim 1 , wherein the forcibly injecting a fungal and nutrient mixture via the one or more orifices into the subsurface zone comprises:

forcibly injecting a slurry mixture of at least one carbon source inoculated with one or more fungal spores via the one or more orifices into the subsurface zone.

8. The process of claim 1 , wherein the forcibly injecting a fungal and nutrient mixture via the one or more orifices into the subsurface zone comprises:

forcibly injecting a liquid mixture containing at least one fungal spore and one or more wood particles via the one or more orifices into the subsurface zone.

9. The process of claim 1 , wherein the forcibly injecting a fungal and nutrient mixture via the one or more orifices into the subsurface zone comprises:

forcibly injecting a liquid mycelium and nutrient mixture via the one or more orifices into the subsurface zone.

10. The process of claim 1 , wherein the forcibly injecting a fluid via the one or more orifices to initiate fracturing in the subsurface zone comprises:

forcibly injecting a fluid at a first pressure via the one or more orifices to initiate fracturing in the subsurface zone.

11. The process of claim 10 , wherein the forcibly injecting a fungal and nutrient mixture via the one or more orifices into the subsurface zone comprises:

forcibly injecting a fungal and nutrient mixture via the one or more orifices into the subsurface zone at a second pressure, the second pressure being lower than the first pressure.

12. The process of claim 1 , further comprising:

injecting a sugar solution at a second location to encourage migration of fungus, the second location being different from a location of injection of the fungal and nutrient mixture.

13. The process of claim 1 , wherein the ventilating the subsurface zone with air via the one or more orifices to support a hyphal network of mycelium in the subsurface zone comprises:

injecting oxygen via the one or more orifices into the subsurface zone to ventilate and encourage growth of mycelium.

14. The process of claim 1 , further comprising:

driving the one or more rod casings deeper within the subsurface zone; and

forcibly injecting the fungal and nutrient mixture via the one or more orifices deeper within the subsurface zone.

15. A process for in-situ mycoremediation, the process comprising:

driving one or more rod casings into a subsurface zone, the one or more rod casings including one or more orifices that are covered;

partially retracting the one or more rod casings to expose the one or more orifices;

forcibly injecting a fungal mixture via the one or more orifices into the subsurface zone to cause fracturing; and

ventilating the subsurface zone via the one or more orifices to support a hyphal network of mycelium in the subsurface zone.

Assignments (5)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 30, 2026
From: SEAS GEOSCIENCES, LLC
To: GREGG DRILLING, LLC
Reel/Frame 075473/0830 →
SECURITY INTEREST Recorded Jan 31, 2025
From: SEAS GEOSCIENCES, LLC
To: U.S. BANK NATIONAL ASSOCIATION, AS ADMINISTRATIVE AGENT
Reel/Frame 070072/0066 →
CHANGE OF NAME Recorded Dec 29, 2023
From: GREGG MARINE, LLC
To: SEAS GEOSCIENCES, LLC
Reel/Frame 066143/0899 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 11, 2023
From: GREGG DRILLING, LLC
To: GREGG MARINE, LLC
Reel/Frame 064218/0215 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 9, 2023
From: GREGG, JOHN; GREGG, LAURA; GREGG, JAMES
To: GREGG DRILLING, LLC
Reel/Frame 064193/0363 →
Continuity (2)
Provisional Application 63217756 · Jul 1, 2021
Related Publication 20230014538A1 · Jan 19, 2023
References Cited (4)
US 5631160A · Bruso · 1997 [cited by examiner]
US 10907143B2 · Kucharzyk et al. · 2021 [cited by applicant]
WO WO2021228351A1 · 2021 [cited by applicant]
Battelle Memorial Institute 2022; Encapsulating Fungal Enzymes for Safer Oil Remediation case study; case study; downloaded on Jun. 29, 2022, at: https://www.battelle.org/insights/case-studies/case-study-details/encapsu… [cited by applicant]