Chromatographic cassette
A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.
1. An adsorptive device comprising:
a shell including a chamber;
chromatographic media disposed within the chamber;
a distribution network fluidly coupled to the chromatographic media;
an inlet port and an outlet port coupled to the distribution network;
wherein the chamber comprises a lattice;
the chromatographic media comprises adsorptive beads; and
wherein the shell further comprises a stackable shell having a feed distributor for distributing the feed stream across multiple adsorptive devices and an eluent distributor for collection of the eluent stream.
2. An adsorptive device comprising:
a shell including a chamber;
chromatographic media disposed within the chamber;
a distribution network fluidly coupled to the chromatographic media;
an inlet port and an outlet port coupled to the distribution network; and
wherein the chromatographic media comprises one of:
a monolithic block;
a polymeric media;
a hydrogel media;
non-woven media;
a compressible adsorptive media; and
a semi-compressible adsorptive media.
3. The adsorptive device of claim 1 , wherein one of the shell and the lattice is manufactured by at least one of:
3-D printing;
molding;
casting; and
extrusion.
4. The adsorptive device of claim 3 , wherein the 3-D printing comprises FDM printing.
5. An adsorptive device comprising:
a shell including a chamber;
chromatographic media disposed within the chamber;
a distribution network fluidly coupled to the chromatographic media; and
an inlet port and an outlet port coupled to the distribution network;
wherein the adsorptive device further comprises:
a top planar surface;
a bottom planar surface,
wherein the distribution network comprises a planar distributor and provides a direction of fluid flow through the chromatographic media which is perpendicular to the top and bottom planar surfaces of the adsorptive device; and
wherein the planar distributor comprises one of:
a type-II planar distributor; and
a type-II isoflo planar distributor.
6. The adsorptive device of claim 5 , wherein the planar distributor comprises first and second planal distributors; and
wherein the adsorptive device further comprises a stackable shell having a feed distributor fluidly coupled to the first planar distributor for distributing the feed stream across multiple adsorptive devices and an eluent distributor fluidly coupled to the second planar distributor for collection of the eluent stream.
7. The adsorptive device of claim 5 , wherein the distribution network provides a direction of fluid flow through the chromatographic media which is parallel to the top and bottom planar surfaces of the adsorptive device.
8. The adsorptive device of claim 2 , wherein the adsorptive device further comprises a peripheral seal.
9. The adsorptive device of claim 2 , further comprising a scalable adsorptive holder and the adsorptive device is disposed within the scalable adsorptive device holder.
10. The adsorptive device of claim 2 , wherein the shell has one of a:
a rectangular shape;
a circular shape; and
a trapezoidal shape.
11. The adsorptive device of claim 1 , wherein the shell is a cassette.
12. A chromatographic method to process a liquid flow comprising:
providing an adsorptive device comprising:
a shell including a chamber;
chromatographic media disposed within the chamber;
a distribution network fluidly coupled to the chromatographic media; and
an inlet port and an outlet port coupled to the distribution network;
wherein the adsorptive device further comprises:
a top planar surface;
a bottom planar surface,
wherein the distributor network comprises a planar distributor and provides a direction of fluid flow through the chromatographic media which is perpendicular to the top and bottom planar surfaces of the adsorptive device;
wherein the planar distributor comprises one of:
a type-II planar distributor; and
a type-II isoflo planar distributor; and
stacking the plurality of stackable adsorptive devices within a holder and processing the liquid flow.
13. The chromatographic method to process a liquid flow of claim 12 further comprising operating at a pressure of greater than 100 psi.
14. The method of claim 12 wherein chromatographic media comprises one of:
compressible chromatographic adsorptive media;
semi-compressible chromatographic adsorptive media;
soft chromatographic adsorptive media;
a monolithic block;
a polymeric media;
a hydrogel media;
a compressible adsorptive media;
a semi-compressible adsorptive media polymethylmethacrylate (PMMA);
agarose Protein-A media;
cation exchange media;
anion exchange media;
mixed-mode media;
size-exclusion chromatography (“SEC”) media;
controlled-pore glass (“CPG”) media;
Protein-A beads;
crosslinked, beaded-form of agarose, polysaccharide polymer cation-exchange beads;
crosslinked, beaded-form of agarose, polysaccharide polymer anion-exchange beads;
25 μm cation-exchange beads
75 μm cation-exchange beads; and
30 μm gel anion-exchange beads;
bed comprising:
a plurality of stackable adsorptive devices;
a chromatographic media packed within each of the plurality of stackable.
15. The method of claim 12 , wherein the linear velocity is greater than 500 cm/hr.
16. The method of claim 12 , wherein a residence time in a loading step is less than one minute.