Engineered cyanobacteria with enhanced UV tolerance
A recombinant strain of F. diplosiphon was made by transforming wild type F. diplosiphon with a pGEM-7Zf (+) plasmid containing the photolyase gene (phrA) via electroporation. The recombinant strain was designated B481-ViAnSa and overexpressed the phrA gene to result in enhanced UV tolerance compared to wild type F. diplosiphon.
1. A recombinant Fremyella diplosiphon cyanobacterium comprising at least one expression plasmid comprising the nucleotide sequence corresponding to SEQ. ID. NO. 3, wherein the recombinant Fremyella diplosiphon cyanobacterium has a higher UV tolerance as compared to wild-type Fremyella diplosiphon cyanobacterium.
2. A recombinant Fremyella diplosiphon cyanobacterium comprising at least one expression plasmid comprising the nucleotide sequence corresponding to SEQ. ID. NO. 3, wherein the recombinant Fremyella diplosiphon cyanobacterium is more resistant to UV-induced DNA damage as compared to wild-type Fremyella diplosiphon cyanobacterium.
3. A recombinant Fremyella diplosiphon cyanobacterium comprising at least one expression plasmid comprising the nucleotide sequence corresponding to SEQ. ID. NO. 3, wherein the recombinant Fremyella diplosiphon cyanobacterium shows a higher dsDNA recovery following DNA damage as compared to wild-type Fremyella diplosiphon cyanobacterium.