Granted Patent B2

US 12,741,059 · App. 17/908,283 · Granted Sep 22, 2026

Inventors: Michiya Matsusaki (Suita, JP); Naoko Sasaki (Suita, JP); Shiro Kitano (Tokyo, JP); Shinji Irie (Tokyo, JP)

Assignees: OSAKA UNIVERSITY; TOPPAN HOLDINGS INC.

A61L27/50A61L27/24A61L27/3633A61L27/3695A61L27/38C12N5/0062C12N2513/00C12N2533/54

Abstract

Claims (13)

1 . A method for controlling a Young's modulus of a three-dimensional tissue containing cells and an extracellular matrix, comprising altering an average diameter of a fragmented extracellular matrix to produce the three-dimensional tissue,

wherein the fragmented extracellular matrix consists of collagen;

the altering an average diameter of the fragmented extracellular matrix includes altering the three-dimensional tissue to contain fragmented extracellular matrices having different average diameters while keeping a total concentration of the collagen constant,

the fragmented extracellular matrices having different average diameters include a fragmented extracellular matrix having an average diameter in a range of 20 nm to 1000 nm and a fragmented extracellular matrix having an average diameter in a range of 1000 nm to 30 μm.

2 . The method according to claim 1 , wherein the altering the three-dimensional tissue to contain fragmented extracellular matrices having different average diameters in the three-dimensional tissue include:

increasing the Young's modulus of the three-dimensional tissue by increasing the content of a fragmented extracellular matrix having the average diameter in a range of 20 nm to 1000 nm in the three-dimensional tissue, or

decreasing the Young's modulus of the three-dimensional tissue by decreasing the content of a fragmented extracellular matrix having the average diameter in a range of 20 nm to 1000 nm in the three-dimensional tissue.

3 . The method according to claim 1 , wherein the fragmented extracellular matrix having the average diameter in the range of 1000 nm to 30 um has an average diameter in between 1.5 um and 8.5 um.

4 . The method according to claim 3 , wherein the Young's modulus of the three-dimensional tissue is 100 kPa or more, and

wherein the fragmented extracellular matrix having the average diameter in a range of 20 nm to 1000 nm has an average diameter between 40 nm and 130 nm and has the content of 1.3 weight % or more.

5 . The method according to claim 4 , wherein the Young's modulus of the three-dimensional tissue is 200 kPa or more, and wherein the content of the fragmented extracellular matrix having the average diameter between 40 nm and 130 nm is 2.0 weight % or more.

6 . The method according to claim 1 , wherein the fragmented extracellular matrix having the average diameter in a range of 20 nm to 1000 nm and the fragmented extracellular matrix having the average diameter in a range of 1000 nm to 30 um are used at a ratio of 1:100 to 100:1 in terms of weight.

7 . The method according to claim 1 , wherein the Young's modulus of the three-dimensional tissue is 20 kPa to 250 kPa.

Assignments (2)

Priority Claims (1)

Continuity (1)

References Cited (22)