Granted Patent
B2
US 12,691,452 · App. 17/979,977 · Granted Jul 28, 2026
Devices for improving sample preparation and processing
Inventors:
Yi Xue (Shrewsbury, MA); Huicheng Wang (Watertown, MA); Sepehr Kiani (Watertown, MA); Corey Alicchio (Watertown, MA)
Assignee:
Illumina, Inc.
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Abstract
The invention provides devices for improving assay protocol compliance, reproducibility, and sensitivity. In particular, the devices of the present invention are tailored to certain single-cell sequencing assays, including single-cell RNA sequencing assays that utilize pre-templated instant partitions (PIPs) templates, wherein such devices hold tubes stationary while performing sample preparation, provide reproducible volume removal from tubes, allow for improved magnetic separation of analytes from buffers, and provide reproducible centrifugation of tubes.
Claims (22)
1 . A vortex adapter configured for use with a vortex mixer, said adapter comprising:
a base comprising a proximal portion releasably couplable to a hub of a vortex mixer; and
a tube holder releasably mounted to a distal portion of the base and comprising a plurality of apertures for receiving a plurality of tubes, respectively, wherein said tube holder is movable between a fixed first position in which the tube holder is releasably mounted to the distal portion of the base such that each of said apertures is oriented in a vertical direction relative to the base and a second fixed position in which the tube holder is releasably mounted to the distal portion of the base such that each of said apertures is oriented in a horizontal direction relative to the base.
2 . The vortex adapter of claim 1 , wherein each of the plurality of apertures is shaped and/or sized to retain a respective tube therein and configured to subject any tube therein to vortex forces from a vortex mixer.
3 . The vortex adapter of claim 2 , wherein the apertures are shaped and/or sized to receive and retain a tube comprising of volume of between 0.1 mL and 5 mL.
4 . The vortex adapter of claim 2 , wherein, when the tube holder is in the first position, each of the apertures retains a respective tube received therein in a vertical direction.
5 . The vortex adapter of claim 4 , wherein a longitudinal axis of each tube is orthogonal relative to a surface upon which a vortex mixer is placed.
6 . The vortex adapter of claim 4 , wherein, when the tube holder is in the first position, tubes received and retained within respective apertures are subjected to vertical vortexing upon receipt of vortex forces from the vortex mixer.
7 . The vortex adapter of claim 2 , wherein, when the tube holder is in the second position, each of the apertures retains a respective tube received therein in a horizontal direction.
8 . The vortex adapter of claim 7 , wherein a longitudinal axis of each tube is parallel relative to a surface upon which a vortex mixer is placed.
9 . The vortex adapter of claim 7 , wherein, when the tube holder is in the second position, tubes received and retained within respective apertures are subjected to horizontal vortexing upon receipt of vortex forces from the vortex mixer.
10 . The vortex adapter of claim 1 , wherein the tube holder comprises at least a first set of apertures arranged in a row.
11 . The vortex adapter of claim 10 , wherein the tube holder comprises a single set of apertures arranged in a row and provided on one side of the tube holder.
12 . The vortex adapter of claim 11 , wherein the tube holder comprises one or more counterweights provided on an opposing side of the tube holder for balancing inertial forces upon receipt of vortex forces from the vortex mixer.
13 . The vortex adapter of claim 10 , wherein the tube holder comprises two sets of apertures, each set arranged in a separate room and provided on opposing sides of the tube holder.
14 . The vortex adapter of claim 1 , further comprising a connection member provided at the distal portion of the base and selectively moveable between an engaged position and a disengaged position.
15 . The vortex adapter of claim 14 , wherein, when in the engaged position, the connection member maintains the tube holder in one of the first and second positions.
16 . The vortex adapter of claim 15 , wherein, when in the disengaged position, the tube holder is moveable between the first and second positions.
17 . The vortex adapter of claim 14 , wherein the distal portion of the base comprises two knuckle members defining a channel therebetween.
18 . The vortex adapter of claim 17 , wherein the connection member comprises an adjustable bolt assembly extending between the knuckle members and configured to draw the knuckle members together upon movement of the bolt assembly to the engaged position.
19 . The vortex adapter of claim 18 , wherein the tube holder comprises a body portion sized to fit within the channel in either of the first and second positions.
20 . The vortex adapter of claim 19 , wherein, when in the engaged position, the connection member causes the knuckle members to apply a retention force upon the body portion of the tube holder and prevent movement thereof within the channel.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 5, 2024
From: FLUENT BIOSCIENCES INC.
To: ILLUMINA, INC.
Reel/Frame 068496/0735 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 20, 2024
From: XUE, YI; WANG, HUICHENG; KIANI, SEPEHR; ALICCHIO, COREY
To: FLUENT BIOSCIENCES, INC.
Reel/Frame 067462/0137 →
Continuity (2)
Provisional Application
63275152
· Nov 3, 2021
Related Publication
20230136755A1
· May 4, 2023
References Cited (267)
US 4202634A
· Kraft et al.
· 1980
[cited by applicant]
US 4305668A
· Bilbrey
· 1981
[cited by applicant]
US 4553955A
· Lam
· 1985
[cited by examiner]
US 4701415A
· Dutton et al.
· 1987
[cited by applicant]
US 4883644A
· Perlman
· 1989
[cited by applicant]
US 5036989A
· Carilli
· 1991
[cited by applicant]
US 5512439A
· Hornes et al.
· 1996
[cited by applicant]
US 5632388A
· Morrison et al.
· 1997
[cited by applicant]
US 5813759A
· Gebrian
· 1998
[cited by applicant]
US 6186657B1
· Fuchsbichler
· 2001
[cited by applicant]
US 6210891B1
· Nyren et al.
· 2001
[cited by applicant]
US 6306597B1
· Macevicz
· 2001
[cited by applicant]
US 6309833B1
· Edman et al.
· 2001
[cited by applicant]
US 6828100B1
· Ronaghi
· 2004
[cited by applicant]
US 6833246B2
· Balasubramanian
· 2004
[cited by applicant]
US 6911345B2
· Quake et al.
· 2005
[cited by applicant]
US 7232656B2
· Balasubramanian et al.
· 2007
[cited by applicant]
US 7296924B2
· Czarnek
· 2007
[cited by applicant]
US 7339671B2
· Peng
· 2008
[cited by applicant]
US 7361472B2
· Yguerabide et al.
· 2008
[cited by applicant]
US 7537897B2
· Brenner et al.
· 2009
[cited by applicant]
US 7598035B2
· Macevicz
· 2009
[cited by applicant]
US 7835871B2
· Kain et al.
· 2010
[cited by applicant]
US 7842457B2
· Berka et al.
· 2010
[cited by applicant]
US 7960120B2
· Rigatti et al.
· 2011
[cited by applicant]
US 8012690B2
· Berka et al.
· 2011
[cited by applicant]
US 8629323B2
· Weeks et al.
· 2014
[cited by applicant]
US 8715934B2
· Diehl et al.
· 2014
[cited by applicant]
US 8748102B2
· Berka et al.
· 2014
[cited by applicant]
US 8765380B2
· Berka et al.
· 2014
[cited by applicant]
US 9011777B2
· Beer
· 2015
[cited by applicant]
US 9012390B2
· Holtze et al.
· 2015
[cited by applicant]
US 9085798B2
· Chee
· 2015
[cited by applicant]
US 9260751B2
· Diehl et al.
· 2016
[cited by applicant]
US 9388465B2
· Hindson et al.
· 2016
[cited by applicant]
US 9399797B2
· Hutchison et al.
· 2016
[cited by applicant]
US 9562837B2
· Link
· 2017
[cited by applicant]
US 9567645B2
· Fan et al.
· 2017
[cited by applicant]
US 9567646B2
· Fan et al.
· 2017
[cited by applicant]
US 9580736B2
· Tan et al.
· 2017
[cited by applicant]
US 9598736B2
· Fan et al.
· 2017
[cited by applicant]
US 9637799B2
· Fan et al.
· 2017
[cited by applicant]
US 9650629B2
· Froehlich et al.
· 2017
[cited by applicant]
US 9695392B2
· Sherman et al.
· 2017
[cited by applicant]
US 9695474B2
· Johnson et al.
· 2017
[cited by applicant]
US 9701998B2
· Hindson et al.
· 2017
[cited by applicant]
US 9708654B2
· Hunicke-Smith et al.
· 2017
[cited by applicant]
US 9783847B2
· Chee
· 2017
[cited by applicant]
US 9951386B2
· Hindson et al.
· 2018
[cited by applicant]
US 20020132251A1
· Shuber
· 2002
[cited by applicant]
US 20030143599A1
· Makarov et al.
· 2003
[cited by applicant]
US 20030180737A1
· Gu et al.
· 2003
[cited by applicant]
US 20040005585A1
· Bi et al.
· 2004
[cited by applicant]
US 20050051466A1
· Carter et al.
· 2005
[cited by applicant]
US 20060024681A1
· Smith et al.
· 2006
[cited by applicant]
US 20060177836A1
· McKernan et al.
· 2006
[cited by applicant]
US 20060177936A1
· Shneider
· 2006
[cited by examiner]
US 20060292611A1
· Berka et al.
· 2006
[cited by applicant]
US 20070080316A1
· Sauer et al.
· 2007
[cited by applicant]
US 20070114362A1
· Feng et al.
· 2007
[cited by applicant]
US 20080004436A1
· Tawfik et al.
· 2008
[cited by applicant]
US 20080056059A1
· Manera et al.
· 2008
[cited by applicant]
US 20090238727A1
· Sinclair et al.
· 2009
[cited by applicant]
US 20090280475A1
· Pollack et al.
· 2009
[cited by applicant]
US 20110009278A1
· Kain et al.
· 2011
[cited by applicant]
US 20110086780A1
· Colston, Jr. et al.
· 2011
[cited by applicant]
US 20110118151A1
· Eshoo et al.
· 2011
[cited by applicant]
US 20110176976A1
· Ebi et al.
· 2011
[cited by applicant]
US 20110311978A1
· Makarewicz, Jr. et al.
· 2011
[cited by applicant]
US 20120180941A1
· Piramoon et al.
· 2012
[cited by applicant]
US 20120295269A1
· Pourahmadi et al.
· 2012
[cited by applicant]
US 20120316074A1
· Saxonov
· 2012
[cited by applicant]
US 20130115169A1
· Lahann et al.
· 2013
[cited by applicant]
US 20140024020A1
· Tanabe
· 2014
[cited by applicant]
US 20140155295A1
· Hindson et al.
· 2014
[cited by applicant]
US 20140293735A1
· Vidakovic et al.
· 2014
[cited by applicant]
US 20140309795A1
· Norton et al.
· 2014
[cited by applicant]
US 20150133312A1
· Bielas et al.
· 2015
[cited by applicant]
US 20150225777A1
· Hindson et al.
· 2015
[cited by applicant]
US 20150232942A1
· Abate et al.
· 2015
[cited by applicant]
US 20160121325A1
· Masquelier et al.
· 2016
[cited by applicant]
US 20160186267A1
· So et al.
· 2016
[cited by applicant]
US 20160250608A1
· Anders et al.
· 2016
[cited by applicant]
US 20160266015A1
· Loo et al.
· 2016
[cited by applicant]
US 20160274103A1
· Piloto et al.
· 2016
[cited by applicant]
US 20170073280A1
· Jones
· 2017
[cited by applicant]
US 20170153248A1
· Goix et al.
· 2017
[cited by applicant]
US 20170165670A1
· Harder et al.
· 2017
[cited by applicant]
US 20170189909A1
· Lee et al.
· 2017
[cited by applicant]
US 20170192030A1
· Lapham et al.
· 2017
[cited by applicant]
US 20170218437A1
· Seul et al.
· 2017
[cited by applicant]
US 20170232417A1
· Lebofsky et al.
· 2017
[cited by applicant]
US 20170255160A1
· Numata et al.
· 2017
[cited by applicant]
US 20170269112A1
· Gerstel
· 2017
[cited by applicant]
US 20180010105A1
· Rogers et al.
· 2018
[cited by applicant]
US 20180051321A1
· Hindson et al.
· 2018
[cited by applicant]
US 20180119216A1
· Jamshidi et al.
· 2018
[cited by applicant]
US 20180133715A1
· Craig et al.
· 2018
[cited by applicant]
US 20180179553A1
· Watson et al.
· 2018
[cited by applicant]
US 20180216162A1
· Belhocine et al.
· 2018
[cited by applicant]
US 20180237836A1
· Abate et al.
· 2018
[cited by applicant]
US 20180274027A1
· Hindson et al.
· 2018
[cited by applicant]
US 20180340880A1
· Matsumoto et al.
· 2018
[cited by applicant]
US 20180355407A1
· Utharala et al.
· 2018
[cited by applicant]
US 20190030539A1
· Vermaas et al.
· 2019
[cited by applicant]
US 20190039034A1
· Siow et al.
· 2019
[cited by applicant]
US 20190085412A1
· Fan et al.
· 2019
[cited by applicant]
US 20190153532A1
· Bharadwaj et al.
· 2019
[cited by applicant]
US 20190153550A1
· Steinmetzer et al.
· 2019
[cited by applicant]
US 20190177789A1
· Hindson et al.
· 2019
[cited by applicant]
US 20190323091A1
· Bramlett et al.
· 2019
[cited by applicant]
US 20190381497A1
· Di Carlo et al.
· 2019
[cited by applicant]
US 20200061618A1
· Lee et al.
· 2020
[cited by applicant]
US 20200080112A1
· Zhang et al.
· 2020
[cited by applicant]
US 20200190513A1
· Fernandez et al.
· 2020
[cited by applicant]
US 20200261879A1
· Abate et al.
· 2020
[cited by applicant]
US 20200324287A1
· Vijayan et al.
· 2020
[cited by applicant]
US 20200376488A1
· Wu et al.
· 2020
[cited by applicant]
US 20210010070A1
· Schnall-Levin et al.
· 2021
[cited by applicant]
US 20210054369A1
· Meltzer et al.
· 2021
[cited by applicant]
US 20210214721A1
· Fontanez et al.
· 2021
[cited by applicant]
US 20210214763A1
· Fontanez et al.
· 2021
[cited by applicant]
US 20210214769A1
· Fontanez et al.
· 2021
[cited by applicant]
US 20210214802A1
· Fontanez et al.
· 2021
[cited by applicant]
US 20210340596A1
· Meltzer et al.
· 2021
[cited by applicant]
US 20220135966A1
· Meltzer
· 2022
[cited by applicant]
US 20220136071A1
· Meltzer
· 2022
[cited by applicant]
US 20220143615A1
· Medoro
· 2022
[cited by applicant]
US 20220154248A1
· Abate et al.
· 2022
[cited by applicant]
US 20220234043A1
· Tanabe et al.
· 2022
[cited by applicant]
US 20220235416A1
· Fontanez et al.
· 2022
[cited by applicant]
US 20220267761A1
· Fontanez et al.
· 2022
[cited by applicant]
AU 2013203624A1
· 2013
[cited by applicant]
CN 112410213A
· 2021
[cited by applicant]
CN 113274927A
· 2021
[cited by applicant]
EP 1441225A1
· 2004
[cited by applicant]
EP 1767275A1
· 2007
[cited by examiner]
EP 1839756A1
· 2007
[cited by applicant]
EP 1877170B1
· 2010
[cited by applicant]
EP 3819637A1
· 2021
[cited by applicant]
JP 2003080115A
· 2003
[cited by applicant]
WO WO9708547A1
· 1997
[cited by applicant]
WO WO2009114619A1
· 2009
[cited by applicant]
WO WO2010117620A2
· 2010
[cited by applicant]
WO WO2011156084A1
· 2011
[cited by applicant]
WO WO2012116146A1
· 2012
[cited by applicant]
WO WO2014100434A1
· 2014
[cited by applicant]
WO WO2014146025A1
· 2014
[cited by applicant]
WO WO2014153071A1
· 2014
[cited by applicant]
WO WO2015157369A1
· 2015
[cited by applicant]
WO WO2015187792A1
· 2015
[cited by applicant]
WO WO2016025815A1
· 2016
[cited by applicant]
WO WO2016040476A1
· 2016
[cited by applicant]
WO WO2016126871A2
· 2016
[cited by applicant]
WO WO2016138080A1
· 2016
[cited by applicant]
WO WO2016172373A1
· 2016
[cited by applicant]
WO WO2017161306A1
· 2017
[cited by applicant]
WO WO2017201451A1
· 2017
[cited by applicant]
WO WO2019011971A1
· 2019
[cited by applicant]
WO WO2019023627A1
· 2019
[cited by applicant]
WO WO2019050840A1
· 2019
[cited by applicant]
WO WO2019204229A1
· 2019
[cited by applicant]
WO WO2019217552A1
· 2019
[cited by applicant]
WO WO2019222523A2
· 2019
[cited by applicant]
WO WO2020037214A1
· 2020
[cited by applicant]
WO WO2020069268A1
· 2020
[cited by applicant]
WO WO2020156879A1
· 2020
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2022/048785, Mar. 17, 2023, 9 pages.
[cited by applicant]
Berensmeier S., “Magnetic Particles for the Separation and Purification of Nucleic Acids”, Applied Microbiology and Biotechnology, Dec. 2006, vol. 73, pp. 495-504.
[cited by applicant]
BIOCOMPARE: “How to Maintain a Constant Temp in Your CO2 Incubator”, Jan. 17, 2013, 7 Pages.
[cited by applicant]
Bowman S.K., et al., “Multiplexed Illumina Sequencing Libraries from Picogram Quantities of DNA”, BMC Genomics, vol. 14, No. 466, Jul. 9, 2013, 8 Pages.
[cited by applicant]
Brouzes E., et al., “Droplet Microfluidic Technology for Single-cell High-throughput Screening”, Proceedings of the National Academy of Sciences of the United States of America, vol. 106, No. 34, Aug. 25, 2009, pp. 1419…
[cited by applicant]
Cai B., et al., “Selection of DNA-Encoded Libraries to Protein Targets within and on Living Cells”, Journal of the American Chemical Society, Oct. 15, 2019, vol. 141, No. 43, pp. 17057-17061, XP093025492, DOI: 10.1021/j…
[cited by applicant]
Cheng W-C., et al., “Ultra-senstive And Rapid Detection of Nucleic Acids and Microorganisms in Body Fluids Using Single-molecule Tethering”, Nature Communications, vol. 11, No. 1, Sep. 22, 2020, pp. 1-9.
[cited by applicant]
Datlinger P., et al., “Pooled CRISPR Screening with Single-Cell Transcriptome Readout”, Author Manuscript, Nature Methods, Mar. 2017, vol. 14, No. 3, pp. 297-301, 20 Pages.
[cited by applicant]
Eastburn D.J., et al., “Ultrahigh-throughput Mammalian Single-cell Reverse-transcriptase Polymerase Chain Reaction in Microfluidic Drops”, Analytical Chemistry, vol. 85, Aug. 20, 2013, pp. 8016-8021.
[cited by applicant]
Fan J., “Barcode Doublets”, JEFWorks Lab, 2017, pp. 1-5.
[cited by applicant]
Figueiredo A., et al., “Cost Effective Method for Construction of High Quality cDNA Libraries”, Biomolecular Engineering, Oct. 2007, vol. 24, pp. 419-421.
[cited by applicant]
Fu Y., et al., “Uniform and Accurate Single-cell Sequencing Based on Emulsion Whole-genome Amplification”, PNAS, Sep. 22, 2015, vol. 112, No. 38, pp. 11923-11928.
[cited by applicant]
Hatori M.N., et al., “Particle-Templated Emulsification for Microfluidics-Free Digital Biology”, Analytical Chemistry, vol. 90, No. 16, Jul. 23, 2018, pp. 9813-9820, XP093011040, the whole document, ISSN: 0003-2700, DOI…
[cited by applicant]
“High Containment Laboratories at CDC—Fifty Years of Excellence”, Centers for Disease Control and Prevention, Last Reviewed: Aug. 9, 2019, 1 Page.
[cited by applicant]
International Patent Application No. PCT/US2021/013042, filed Jan. 12, 2021, International Search Report and Written Opinion, issued Mar. 29, 2021, 12 Pages.
[cited by applicant]
International Patent Application No. PCT/US2021/013048, filed Jan. 12, 2021, International Search Report and Written Opinion, issued Mar. 31, 2021, 18 Pages.
[cited by applicant]
International Patent Application No. PCT/US2021/013065, filed Jan. 12, 2021, International Search Report and Written Opinion issued Mar. 29, 2021, 15 pages.
[cited by applicant]
International Patent Application No. PCT/US2021/013066, filed Jan. 12, 2021, International Search Report and Written Opinion, issued Mar. 29, 2021, 13 Pages.
[cited by applicant]
International Patent Application No. PCT/US2021/013069, filed Jan. 12, 2021, International Search Report and Written Opinion issued Apr. 1, 2021, 13 Pages.
[cited by applicant]
International Patent Application No. PCT/US2021/022503, filed Mar. 16, 2021, International Search Report and Written Opinion, issued Aug. 11, 2021, 12 Pages.
[cited by applicant]
International Patent Application No. PCT/US2022/037078, filed Jul. 14, 2022, International Search Report and Written Opinion issued Oct. 12, 2022, 21 pages.
[cited by applicant]
International Patent Application No. PCT/US2022/037129, filed Jul. 14, 2022, International Search Report and Written Opinion issued Oct. 11, 2022, 16 Pages.
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2020/47214, mailed Feb. 3, 2021, 19 Pages.
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2021/013045, mailed Mar. 29, 2021, 10 Pages.
[cited by applicant]
International Search Report and Written Opinion for International Application No. PCT/US2021/023815, mailed Jun. 30, 2021, 19 Pages.
[cited by applicant]
Jacobsen N., et al., “Direct Isolation of poly(A)+ RNA from 4 M Guanidine Thiocyanate-lysed Cell Extracts Using Locked Nucleic Acid-oligo(T) Capture”, Nucleic Acids Research, vol. 32, No. 7, Apr. 19, 2004, pp. 1-10.
[cited by applicant]
Karlsson K., “Counting Molecules in Cell-free DNA and Single Cells RNA”, Thesis, Division of Molecular Neurobiology Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 2016, 52 Pages.
[cited by applicant]
Kim S.C., et al., “Single-Cell RT-PCR in Microfluidic Droplets with Integrated Chemical Lysis”, Analytical chemistry, vol. 90, No. 2, Jan. 16, 2018, pp. 1273-1279, 13 Pages.
[cited by applicant]
Klein A.M., et al., “Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells”, Cell, Amsterdam, NL, May 21, 2015, vol. 161, No. 5, pp. 1187-1201, 16 Pages, XP055731640.
[cited by applicant]
Kukurba K.R., et al., “RNA Sequencing and Analysis”, Cold Spring Harb Protoc, vol. 11, Apr. 13, 2015, pp. 951-969.
[cited by applicant]
Kumaresan P., et al., “High-throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets”, Analytical Chemistry, May 15, 2008, vol. 80, pp. 3522-3529.
[cited by applicant]
Lage J.M., et al., “Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array-CGH”, Genome Research, Feb. 2003, vol. 13, No. 2, pp. 294-307.
[cited by applicant]
Lyons E., et al., “Large-scale DNA Barcode Library Generation for Biomolecule Identification in High-throughput Screens”, Scientific Reports, vol. 7, No. 13899, Oct. 2017, pp. 1-7.
[cited by applicant]
Markus H., et al., “Analysis of Recurrently Protected Genomic Regions in cell-free DNA Found in Urine”, Science Translational Medicine, vol. 13, No. 581, Feb. 17, 2021, pp. 1-31.
[cited by applicant]
Mazutis L., et al., “Single-cell Analysis and Sorting Using Droplet-based Microfluidics”, Nature Protocols, May 2013, vol. 8, No. 5, pp. 870-891.
[cited by applicant]
Nishikawa Y., et al., “Monodisperse Picoliter Droplets for Low-bias and Contamination-free Reactions in Single-cell Whole Genome Amplification”, PLOS One, vol. 10, No. 9, e0138733, Sep. 21, 2015, pp. 1-15.
[cited by applicant]
Patel A.K., et al., “Design and Fabrication of Low Cost Vortex Mixer Using Additive Manufacturing”, International Journal of Applied Engineering Research, vol. 14, No. 1, 2019, pp. 246-249.
[cited by applicant]
Petersen L.K., et al., “Screening of DNA-encoded Small Molecule Libraries Inside a Living Cell”, Journal of the American Shemical Society, vol. 143, No. 7, Feb. 24, 2021, pp. 2751-2756.
[cited by applicant]
Quail M.A., et al., “A tale of Three Next Generation Sequencing Platforms: Comparison of Ion Torrent, Pacific Biosciences and Ilumina MiSeq Sequencers”, BMC Genomics, vol. 13, No. 341, Jul. 24, 2012, 13 Pages.
[cited by applicant]
Replogle J.M., et al., “Combinatorial single-cell CRISPR Screens by Direct Guide RNA Capture and Targeted Sequencing”, Nature Biotechnology, vol. 38, No. 8, Aug. 2020, pp. 954-961.
[cited by applicant]
Roche., “EmPCR Amplificaiotn Method Manual”, 454 Life Sciences Corp, 2011, 12 Pages.
[cited by applicant]
Sidore A.M., et al., “Enhanced Sequencing Coverage with Digital Droplet Multiple Displacement Amplification”, Nucleic Acids Research, vol. 44, No. 7, e66, Apr. 20, 2016, pp. 1-9.
[cited by applicant]
Stoeckius M., et al., “Simultaneous Epitope and Transcriptome Measurment in Single Cells”, Nature Methods, online publication, vol. 14, No. 9, Sep. 2017, 10 pages.
[cited by applicant]
Stoeckius M., et al., “Large-Scale Simultaneous Measurement of Epitopes and Transcriptomes in Single Cells”, Nature Methods, Sep. 2017, vol. 14, No. 9, pp. 865-868, 17 Pages.
[cited by applicant]
Suh S.K., et al., “Synthesis of Magnetic Hydrogel Microparticles for Bioassays and Tweezer Manipulation in Microwells”, Microfluid ganofluid, vol. 13, 2012, pp. 655-674.
[cited by applicant]
Tamminen M.V., et al., “Single Gene-based Distinction of Individual Microbial Genomes from a Mixed Population of Microbial Cells”, Front Microbiology, vol. 6, No. 195, Mar. 2015, pp. 1-10.
[cited by applicant]
Tokunaga T., et al., “Systematic Exploration of Lipophilic Tags That Allow Efficient Anchoring of Aptamers to Live Cell Surfaces”, Chemistry Letters, vol. 42, No. 2, Feb. 2013, pp. 127-129.
[cited by applicant]
Vitale S.R., et al., “An Optimized Workflow to Evaluate Estrogen Receptor Gene Mutations in Small Amounts of Cell-Free DNA”, The Journal of Molecular Diagnostics, vol. 21, No. 1, Jan. 2019, pp. 123-137.
[cited by applicant]
Walls A.C., et al., “Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glcoprotein”, Cell, Apr. 16, 2020, vol. 180, No. 2, pp. 281-292, 20 Pages.
[cited by applicant]
Zilionis R., et al., “Single-cell Barcoding and Sequencing Using Droplet Microfluidics”, Nature Protocols, vol. 12, No. 1, Dec. 8, 2016, pp. 44-73, XP055532179, XP055367764, figure 1, p. 46, 47, 50, 52.
[cited by applicant]
Kumari S., et al., “Quantification of Circulating Free DNA as a Diagnostic Marker in Gall Bladder Cancer”, Cancer, Pathology & Oncology Research, Jan. 2017, vol. 23, No. 1, pp. 91-97.
[cited by applicant]
Quail M.A., et al., “A Tale of Three Next Generation Sequencing Platforms: Comparison of Ion Torrent, Pacific Biosciences and Ilumina MiSeq Sequencers”, BMC Genomics, vol. 13, No. 341, Jul. 24, 2012, pp. 1-13.
[cited by applicant]