Granted Patent B2

US 12,635,694 · App. 17/984,485 · Granted May 26, 2026

Inventor: Tony Hagen (Sioux Falls, SD)

A01N63/27A01N63/20A01N63/22A01N63/32C12N1/20

Abstract

Claims (22)

1 . A microbial inoculant composition comprising one or more of:

Bacillus thuringiensis, Bacillus popilliae, Photorhabdus luminescens, Beauveria bassiana , or Lagenidium giganteum ; and

Clostridium saccharobutylicum .

2 . The microbial inoculant composition of claim 1 , further comprising Pseudomonas moraviensis or Pseudomonas fluorescens.

3 . The microbial inoculant composition of claim 1 , further comprising Bacillus megaterium, Bacillus subtilis , or Bacillus licheniformis.

4 . The microbial inoculant composition of claim 1 , further comprising an aquatic Delftia species pluralis.

5 . The microbial inoculant composition of claim 1 , further comprising an aquatic Chryseobacterium species pluralis.

6 . The microbial inoculant composition of claim 1 , further comprising Brevundimonas kwangchunensis, Fictibacillus barbaricus/Bacillus barbaricus , a Prosthecobacter species pluralis, Sphingobacterium multivorum , or a Sphingomonas species pluralis.

7 . The microbial inoculant composition of claim 1 , further comprising Bacillus megaterium, Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus pumilus, Sphingosinicella microcystinivorans, Pseudomonas chlororaphis, Pseudomonas mandelii, Pseudomonas umsongensis , a Clostridium species pluralis, Arthrobacter ramosus, Streptomyces yogyakartensis , an Arthrobacter species pluralis, a Xanthomonas species pluralis, or Chryseobacterium indologenes .

8 . The microbial inoculant composition of claim 1 , further comprising a yeast strain.

9 . The microbial inoculant composition of claim 1 , further comprising one or more non-microbial additives, wherein the one or more non-microbial additives are carbon, nitrogen, potassium, phosphorus, zinc, magnesium, selenium, chromium, tin, manganese, cobalt, zinc, copper, or a combination thereof.

10 . A plant comprising the microbial inoculant composition of claim 1 adhered to at least a portion of the plant.

11 . A seed comprising the microbial inoculant composition of claim 1 adhered to at least a portion of the seed.

12 . A method comprising applying the microbial inoculant composition of claim 1 to a tissue of a plant.

13 . A method comprising applying the microbial inoculant composition of claim 1 to a surface of a seed.

14 . A method comprising applying the microbial inoculant composition of claim 1 to a seed bed.

15 . A method comprising applying the microbial inoculant composition of claim 1 to a field comprising a plurality of plants.

16 . A method of producing a microbial inoculant composition, the method comprising: providing microbes comprising one or more of:

Bacillus thuringiensis, Bacillus popilliae, Photorhabdus luminescens, Beauveria bassiana , or Lagenidium giganteum and Clostridium saccharobutylicum ; providing culture medium comprising: a carbon source; sufficient vinegar to adjust the pH to no higher than 6.8; iron at a concentration of 1 ppm; and potassium nitrate;

incubating the microbes together in the culture medium under conditions effective to allow aerobic fermentation and anaerobic fermentation.

17 . The method of claim 16 , wherein the microbes are incubated at a temperature of at least 15° C.

18 . The method of claim 16 or claim 17 , wherein the microbes are incubated for at least five days.

Assignments (1)

Continuity (6)

References Cited (24)