IP Library › Granted Patent US 11,980,183
Granted Patent B2
US 11,980,183 · App. 16/635,149 · Granted May 14, 2024

Microbial inoculant compositions and methods

Inventor: Tony Hagen (Sioux Falls, SD)
Assignee: RAISON, LLC
A01N25/02A01H5/10A01N57/12A01N59/26
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Quick Facts
Patent No.
US 11,980,183
App. No.
16/635,149
Granted
May 14, 2024
Kind
B2
Abstract

A microbial inoculant composition includes aquatic bacterial species. In some embodiments, the microbial inoculant composition includes at least one of an aquatic Pseudomonas spp. and a Clostridium spp.

Claims (34)

1. A microbial inoculant composition comprising:

an aquatic Pseudomonas spp.;

an aquatic Delftia spp.;

a Clostridium spp.;

Sphingosinicella microcystinivorans ; and

Pseudomonas chlororaphis, Pseudomonas mandelii, Pseudomonas umsongensis, Arthrobacter ramosus , or Streptomyces yogyakartensis.

2. The microbial inoculant composition of claim 1 , wherein the aquatic Pseudomonas spp. comprises P. moraviensis or P. fluorescens.

3. The microbial inoculant composition of claim 1 , wherein the Clostridium spp. comprises Clostridium saccharobutylicum.

4. The microbial inoculant composition of claim 1 , further comprising a Bacillus spp.

5. The microbial inoculant composition of claim 4 , wherein the Bacillus spp. comprises B. megaterium, B. subtilis , or B. licheniformis.

6. The microbial inoculant composition of claim 1 , further comprising an aquatic Chryseobacterium spp.

7. The microbial inoculant composition of claim 1 , further comprising an aquatic Pseudomonas fluorescens.

8. The microbial inoculant composition of claim 1 , further comprising Brevundimonas kwangchunensis, Fictibacillus barbaricus/Bacillus barbaricus , a Prosthecobacter spp., Sphingobacterium multivorum , or a Sphingomonas spp.

9. The microbial inoculant composition of claim 1 , further comprising an Arthrobacter spp.

10. The microbial inoculant composition of claim 1 further comprising a yeast strain.

11. A plant comprising the microbial inoculant composition of claim 1 adhered to at least a portion of the plant.

12. A seed comprising the microbial inoculant composition of claim 1 adhered to at least a portion of the seed.

13. The microbial inoculant composition of claim 1 , further comprising Bacillus megaterium, Bacillus amyloliquifaciens, Bacillus subtilus, Bacillus pumilus , a Xanthomonas spp., or Chryseobacterium indologenes.

14. The microbial inoculant composition of claim 1 , wherein the microbial inoculant is present on a terrestrial plant.

15. A method comprising applying the microbial inoculant composition of claim 1 to a tissue of a plant.

16. A method comprising applying the microbial inoculant composition of claim 1 to a surface of a seed.

17. A method comprising applying the microbial inoculant composition of claim 1 to a seed bed.

18. A method comprising applying the microbial inoculant composition of claim 1 to a field comprising a plurality of plants.

19. A method of producing a microbial inoculant composition, the method comprising:

providing the microbes recited in claim 1 ;

providing culture medium comprising:

a carbon source;

sufficient vinegar to adjust the pH to no higher than 6.8;

iron at a concentration of 1 ppm; and

potassium nitrate;

and

incubating the microbes in the culture medium under conditions effective to allow aerobic fermentation and anaerobic fermentation.

20. The method of claim 19 , wherein the microbes are incubated at a temperature of at least 15° C.

21. The method of claim 19 , wherein the microbes are incubated for at least five days.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 29, 2021
From: HAGEN, TONY
To: RAISON, LLC
Reel/Frame 055756/0854 →
Continuity (5)
Provisional Application 62672190 · May 16, 2018
Provisional Application 62663067 · Apr 26, 2018
Provisional Application 62660836 · Apr 20, 2018
Provisional Application 62541422 · Aug 4, 2017
Related Publication 20210084895A1 · Mar 25, 2021