Fluid connector
Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.
1. A system for processing cell products in parallel comprising:
a sterile enclosure;
at least one robotic arm positioned within the sterile enclosure, the at least one robotic arm attached to a linear rail;
a first cartridge including a port and a liquid transfer bus, wherein the first cartridge is capable of receiving a cell product to be processed and of interfacing with one or more instruments;
a first instrument including a first slot positioned within the sterile enclosure, the first instrument comprising a sterile liquid transfer instrument configured to transfer fluid in and out of the first cartridge via the port; and
a second instrument including a second slot positioned within the sterile enclosure, the second instrument comprising a bioreactor instrument configured to enable expansion of cells within the first cartridge,
wherein the at least one robotic arm is configured to move the first cartridge between the first slot within the first instrument and the second slot within the second instrument in order to process a cell product within the first cartridge.
2. The system of claim 1 , further comprising a second cartridge positioned within the sterile enclosure, wherein the system is capable of processing a cell product within the second cartridge and the cell product within the first cartridge in parallel.
3. The system of claim 1 , wherein the at least one robotic arm includes two robotic arms positioned within the sterile enclosure.
4. The system of claim 1 , wherein the sterile enclosure comprises an air filtration inlet that provides ISO8 or better air quality within the sterile enclosure.
5. The system of claim 1 , wherein the sterile enclosure comprises a feedthrough configured to initially receive the first cartridge, the feedthrough being capable of sterilizing the first cartridge via ultraviolet radiation (UV) or chemical sterilizing agents.
6. The system of claim 1 , wherein the system is capable of generating electronic batch records based on process parameters and data collected from sensors during cell process execution within the system.
7. The system of claim 1 , further comprising one or more processors for automatically adjusting cell process steps within the system according to a measurement of cell process parameters or results including cell number, cell density, cell viability, temperature, dissolved oxygen, pH, glucose levels, and volumes of onboard reagent storage and waste.
8. A system for processing cell products in parallel comprising:
a sterile enclosure;
a pair of robotic arms positioned within the sterile enclosure, each of the robotic arms independently configured for moving one or more cartridges;
a first cartridge including a port a liquid transfer bus, wherein the first cartridge is capable of receiving a cell product to be processed and of interfacing with one or more instruments;
a first instrument including a first slot positioned within the sterile enclosure, the first instrument comprising a sterile liquid transfer instrument configured to transfer fluid in and out of the port of the first cartridge; and
a second instrument including a second slot positioned within the sterile enclosure, the second instrument comprising a bioreactor instrument configured to enable cell expansion within the first cartridge,
wherein at least one of the pair of robotic arms is configured to move the first cartridge from the feedthrough to either of the first slot within the first instrument or the second slot within the second instrument in order to process the cell product within the first cartridge.
9. The system of claim 8 , further comprising a reagent vault for storing one or more reagents.
10. The system of claim 9 , wherein at least one of the pair of robotic arms moves a reagent from the reagent vault to the sterile liquid transfer instrument.
11. The system of claim 8 , wherein at least one of the pair of robotic arms is fixed to a linear rail.
12. The system of claim 8 , wherein the sterile enclosure comprises a feedthrough configured to initially receive the first cartridge, the feedthrough being capable of sterilizing the first cartridge via ultraviolet radiation (UV) or chemical sterilizing agents.
13. The system of claim 8 , wherein the system is capable of generating electronic batch records based on process parameters and data collected from sensors during cell process execution within the system.
14. The system of claim 8 , further comprising one or more processors for automatically adjusting cell process steps within the system according to a measurement of cell process parameters or results including cell number, cell density, cell viability, temperature, dissolved oxygen, pH, glucose levels, and volumes of onboard reagent storage and waste.
15. The system of claim 8 , wherein the cartridge comprises configurable modules.
16. The system of claim 8 , wherein the system is capable of generating several cell products in parallel so as to be combined for a therapeutic use.
17. A system for processing cell products in parallel comprising:
a sterile enclosure including an air filtration inlet and a feedthrough;
at least one robotic arm positioned within the sterile enclosure, the at least one robotic arm attached to a linear rail;
a first cartridge including a port and two or more modules fluidically coupled by a liquid transfer bus, wherein each of the modules is capable of receiving a cell product to be processed, and wherein each of the modules is capable of interfacing with one or more instruments;
a first instrument including a first slot positioned within the sterile enclosure, the first instrument comprising a sterile liquid transfer instrument configured to transfer fluid in and out of the port of the first cartridge; and
a second instrument including a second slot positioned within the sterile enclosure, the second instrument comprising a bioreactor instrument configured to enable cell expansion within the first cartridge,
wherein the at least one robotic arm is configured to move the first cartridge from the feedthrough to either of the first slot within the first instrument or the second slot within the second instrument in order to process a cell product within the first cartridge.
18. The system of claim 17 , further comprising a second cartridge positioned within the sterile enclosure, wherein the system is capable of processing a cell product within the second cartridge with the cell product within the first cartridge in parallel.
19. The system of claim 17 , wherein the at least one robotic arm includes two robotic arms positioned within the sterile enclosure.
20. The system of claim 17 , wherein the air filtration inlet provides ISO8 or better air quality within the sterile enclosure.
21. The system of claim 17 , wherein the feedthrough is capable of sterilizing the first cartridge via ultraviolet radiation (UV) or chemical sterilizing agents.
22. The system of claim 17 , wherein the system is capable of generating electronic batch records based on process parameters and data collected from sensors during cell process execution within the system.
23. The system of claim 17 , further comprising one or more processors for automatically adjusting cell process steps within the system according to a measurement of cell process parameters or results including cell number, cell density, cell viability, temperature, dissolved oxygen, pH, glucose levels, and volumes of onboard reagent storage and waste.
24. The system of claim 17 , wherein the first cartridge comprises configurable modules linked by configurable fluidic, optical, and/or electrical connections.
25. A system for processing cell products in parallel comprising:
a sterile enclosure including an air filtration inlet for maintaining an ISO8 environment or better and a feedthrough;
a pair of robotic arms positioned within the sterile enclosure, each of the robotic arms independently configured for moving one or more cartridges;
a first cartridge including a port and two or more modules fluidically coupled by a liquid transfer bus, wherein each of the modules is capable of receiving a cell product to be processed, and wherein each of the modules is capable of interfacing with one or more instruments;
a first instrument including a first slot positioned within the sterile enclosure, the first instrument comprising a sterile liquid transfer instrument configured to transfer fluid in and out of the port of the first cartridge; and
a second instrument including a second slot positioned within the sterile enclosure, the second instrument comprising a bioreactor instrument configured to enable cell expansion within the first cartridge,
wherein at least one of the pair of robotic arms is configured to move the first cartridge from the feedthrough to either of the first slot within the first instrument or the second slot within the second instrument in order to process a cell product within the first cartridge.
26. The system of claim 25 , further comprising a reagent vault for storing one or more reagents.
27. The system of claim 26 , wherein at least one of the pair of robotic arms moves a reagent from the reagent vault to the sterile liquid transfer instrument.
28. The system of claim 25 , wherein the system is capable of generating electronic batch records based on process parameters and data collected from sensors during cell process execution within the system.
29. The system of claim 25 , further comprising one or more processors for automatically adjusting cell process steps within the system according to a measurement of cell process parameters or results including cell number, cell density, cell viability, temperature, dissolved oxygen, pH, glucose levels, and volumes of onboard reagent storage and waste.