IP Library › Granted Patent US 12,185,732
Granted Patent B2
US 12,185,732 · App. 18/046,466 · Granted Jan 7, 2025

Method for producing a dairy product

Inventors: Hanne Vang Hendriksen (Holte, DK); Steffen Ernst (Broenshoej, DK); Reinhard Wilting (Farum, DK); Jeppe Wegener Tams (Gentofte, DK); Mette Oerhrstroem Runge (Snekkersten, DK); Helle Skov Guldager (Vedbaek, DK)
Assignee: Kerry Group Services International Ltd
A23C9/1206A23C9/123
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,185,732
App. No.
18/046,466
Granted
Jan 7, 2025
Kind
B2
Abstract

The present invention relates to a method for producing a dairy product using an enzyme having lactase activity.

Claims (20)

1. A polypeptide having lactase activity, wherein the polypeptide is at least 90 percent identical to amino acids 28-1331 of SEQ ID NO: 2 having lactase activity, wherein the polypeptide is isolated.

2. The polypeptide of claim 1 , wherein the polypeptide is at least 95 percent identical to amino acids 28-1331 of SEQ ID NO: 2 having lactase activity.

3. The polypeptide of claim 1 , wherein the polypeptide is at least 98 percent identical to amino acids 28-1331 of SEQ ID NO: 2 having lactase activity.

4. The polypeptide of claim 1 , wherein the polypeptide is at least 90 percent identical to amino acids 28-1331 of SEQ ID NO: 2 and consists of 1304 amino acids.

5. The polypeptide of claim 1 , wherein the polypeptide has a ratio of lactase to transgalactosylase activity of more than 1:1 when hydrolysing lactose in a milk-based substrate.

6. A composition comprising the polypeptide of claim 1 .

7. The composition of claim 6 , wherein the composition further comprises a milk-based substrate.

8. The composition of claim 7 , wherein the milk-based substrate comprises lactose.

9. The composition of claim 6 , wherein the composition is a dairy product.

10. The composition of claim 9 , wherein the dairy product is a fermented dairy product.

11. The composition of 10 , wherein the dairy product is yoghurt.

12. A method for producing a food product, comprising treating a milk-based substrate comprising lactose with the polypeptide of claim 1 .

13. A method for producing a dairy product, comprising treating a milk-based substrate comprising lactose with the polypeptide of claim 1 .

14. The polypeptide of claim 1 , wherein the polypeptide is at least 99 percent identical to amino acids 28-1331 of SEQ ID NO: 2 having lactase activity.

15. The polypeptide of claim 1 , wherein the first amino acid in the sequence is isoleucine.

16. The polypeptide of claim 1 , wherein the second amino acid in the sequence is glutamate.

17. The polypeptide of claim 1 , wherein the third amino acid in the sequence is aspartate.

18. The polypeptide of claim 1 , wherein the fourth amino acid in the sequence is alanine.

19. The polypeptide of claim 1 , wherein the fifth amino acid in the sequence is threonine.

20. The polypeptide of claim 1 , wherein the sixth amino acid in the sequence is arginine.

Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 21, 2024
From: RUNGE, METTE O.; GULDAGER, HELLE S.
To: CHR. HANSEN A/S
Reel/Frame 068360/0550 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 21, 2024
From: HENDRIKSEN, HANNE V.; ERNST, STEFFEN; WILTING, REINHARD; TAMS, JEPPE W.
To: NOVOZYMES A/S
Reel/Frame 068360/0922 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 21, 2024
From: CHR. HANSEN A/S
To: NOVOZYMES A/S
Reel/Frame 068360/0993 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 21, 2024
From: NOVOZYMES A/S
To: KERRY GROUP SERVICES INTERNATIONAL LTD
Reel/Frame 067480/0489 →
Priority Claims (2)
EP 07122110 · Dec 3, 2007 · regional
EP 08156674 · May 21, 2008 · regional
Continuity (9)
Continuation 17068015 · Oct 12, 2020
Continuation 16720478 · Dec 19, 2019
Continuation 16380220 · Apr 10, 2019
Continuation 16021048 · Jun 28, 2018
Continuation 15433642 · Feb 15, 2017
Continuation 12744508
Provisional Application 61055164 · May 22, 2008
Provisional Application 60992783 · Dec 6, 2007
Related Publication 20230189830A1 · Jun 22, 2023
References Cited (74)
US 5736374A · Berka et al. · 1998 [cited by applicant]
US 7081355B2 · Jorgensen · 2006 [cited by examiner]
US 10058107B2 · Hendriksen et al. · 2018 [cited by applicant]
US 10306902B2 · Hendriksen · 2019 [cited by examiner]
US 10555541B2 · Hendriksen et al. · 2020 [cited by applicant]
US 20080286412A1 · Dekker et al. · 2008 [cited by applicant]
US 20170156357A1 · Hendriksen et al. · 2017 [cited by applicant]
US 20200120946A1 · Hendriksen et al. · 2020 [cited by applicant]
CN 1652690A · 2005 [cited by applicant]
CN 1807609A · 2006 [cited by applicant]
CN 1810161A · 2006 [cited by applicant]
EP 0026672A2 · 1981 [cited by applicant]
EP 0119329A1 · 1984 [cited by applicant]
EP 0323201A2 · 1989 [cited by applicant]
EP 1283876B1 · 2007 [cited by applicant]
WO 2001090317A2 · 2001 [cited by applicant]
WO 2002081673A1 · 2002 [cited by applicant]
WO 2003094623A1 · 2003 [cited by applicant]
WO 2007021204A1 · 2007 [cited by applicant]
WO 2009071539A1 · 2009 [cited by applicant]
WO 2013182686A1 · 2013 [cited by applicant]
Anonymous, Asia Pacific Food Industry, 2001, 40-43. [cited by applicant]
Anonymous, Danisco US, Inc. and DuPont Nutrition Biosciences ApS, Petition for Inter Partes Review of US 10, 058, 107 (IPR2021-00188), 2020. [cited by applicant]
Anonymous, Danisco US, Inc. and DuPont Nutrition Biosciences ApS, Petition for Inter Partes Review of US 10, 555, 541 (IPR2021-00189), 2020. [cited by applicant]
Anonymous, Food Ingredients Innovation Awards 17, 2017. [cited by applicant]
Anonymous, Multiple alignment. [cited by applicant]
Anonymous, Paranashop announcement re food awards, 2017. [cited by applicant]
Anonymous, Sequence alignments comparison of BIF1331 without HQ in D27 and D37. [cited by applicant]
Arla Whey Permeate brochure. [cited by applicant]
Bornhorst et al., Methods Enzymol., 2000, 245-254, 326. [cited by applicant]
Breuner, Declaration submitted in an opposition against EP2859098, 2019. [cited by applicant]
Broome et al., Australian Journal of Dairy Technology, 1983, 35-37, 38(1). [cited by applicant]
Chr. Hansen, Ha-lactase application sheet, 2010, 1-7. [cited by applicant]
Clark, Declaration of Douglas S. Clark submitted in IPR 2021-00188, 2020, 1-106. [cited by applicant]
Clark, Declaration of Douglas S. Clark submitted in IPR 2021-00189, 2020, 1-106. [cited by applicant]
Cruz et al., Revista de Microbiologia, 1999, 265-271, 30. [cited by applicant]
De Vrese et al., Am J Clin Nutr, 2001, 421-429, 73. [cited by applicant]
Dechter et al., Food Biotechnology, 1998, 73-89, 12. [cited by applicant]
Dumortier et al., Biotechnol. Appl. Biochem., 1994, 341-354, 19. [cited by applicant]
EP2234501 and Uniprotkb access No. U2C011 sequence alignment. [cited by applicant]
Giessing, Declaration submitted in an opposition against EP2859098, 2019. [cited by applicant]
Goulas et al., Appl Microbiol Biotechnol, 2007, 1365-1372, 76(6). [cited by applicant]
Goulas et al., UniProt Access No. A4K5H9, 2007. [cited by applicant]
Hussein et al., Journal of Food Protection, 1988, 30-34, 52(1). [cited by applicant]
Ismail et al., Journal of Society of Dairy Technology, 1983, 52-55, 36(2). [cited by applicant]
Joergensen et al., Appl Microbiol Biotechnol, 2001, 647-652, 57. [cited by applicant]
Jurado et al., Enzyme and Microbial Technology, 2004, 33-40, 34. [cited by applicant]
Kang et al., Enzyme and Microbial Technology, 2005, 655-662, 37. [cited by applicant]
Kenward, Food Ingredients, 2016, 1-2. [cited by applicant]
Ma et al., Science and Technology of Food Industry, 2002, 50-52. [cited by applicant]
Ma et al., Science and Technology of Food Industry, 2002, 50-52,—Translation. [cited by applicant]
Madsen et al., UniProt Access No. Q9F4D5, 2003. [cited by applicant]
Mahoney et al., Journal of Food Science, 1978, 584-591, 43. [cited by applicant]
Marchler-Bauer, NCBI Accession No. CAC14566.2, 2004. [cited by applicant]
Martinez-Villaluenga et al., Food Chemistry, 2008, 258-264, 107(1). [cited by applicant]
Moeller et al., Applied and Environmental Microbiology, 2001, 2276-2283, 67(5). [cited by applicant]
Myers et al., UniProt Access No. Q0TR23, 2006. [cited by applicant]
Novozymes, Application Sheet Lactose Reduction, 2009, 1-7. [cited by applicant]
Passerat et al., Nutrition Research, 1995, 1287-1295, 15(9). [cited by applicant]
Rao, Food Science and Technology International, 1997, 87-92, 3. [cited by applicant]
Rubio-Texeira, Biotechnology Advances, 2006, 212-225, 24. [cited by applicant]
Shimizu et al., UniProt Access No. Q8XKX6, 2002. [cited by applicant]
Sudarsanam et al., UniProt Access No. A5KPK7, 2007. [cited by applicant]
Tamm, Scand J Gastroentero, 1994, 55-63. [cited by applicant]
WO2001090317A2 and EP2234501 Sequence comparison. [cited by applicant]
WO2001090317A2 and EP2234501 Sequence comparison 2. [cited by applicant]
Anonymous, Food Ingredients South America Innovation Awards (2017). [cited by applicant]
Anonymous, Food Ingredients Innovation Awards, “Novozymes is Finalist of the Fi Innovation Awards 2017 with Saphera” (2017). [cited by applicant]
Anonymous, Sequence Alignment between Amino Acid Sequences Disclosed in EP 2234501 and WO 01/90317 (date unknown). [cited by applicant]
Anonymous, Alignment of BIF 1331 with QH-tag, BIF 1331 without QH-tag, and Seq ID No. 2 of EP 2234501 (date unknown). [cited by applicant]
Anonymous, Alignment of BIF-1331 without HA (date unknown). [cited by applicant]
Anonymous, Alignment of Seq ID No. 2 of EP 2234 501 and B. bifidum ATCC 29521 (UniProtKB Acession No. U2CO11) (date unknown). [cited by applicant]
Kenward, “Novozymes: Saphera Enables New Innovation in Lactose-Free, Fermented Dairy Products” (2016). [cited by applicant]
Madsen et al., GenBank Accession CAC14566.2 (2003). [cited by applicant]