Granted Patent
B2
US 12,708,656 · App. 18/056,823 · Granted Aug 18, 2026
Peptide-polynucleotide-hyaluronic acid nanoparticles and methods for polynucleotide transfection
Inventors:
Samuel A. Wickline (Temple Terrace, FL); Hua Pan (Tampa, FL); Christine Thien-Nga Pham (St. Louis, MO); Huimin Yan (St. Louis, MO)
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Abstract
The present disclosure relates to nanoparticles and methods for polynucleotide transfection.
Claims (19)
1 . A pharmaceutical composition comprising a peptide-polynucleotide complex, the peptide-polynucleotide complex comprising:
a peptide;
a polynucleotide; and
hyaluronic acid;
wherein the peptide comprises an amino acid sequence which is at least 95% identical to the full-length amino acid sequence of SEQ ID NO: 1; and
wherein the peptide comprises one conservative amino acid substitution.
2 . The composition of claim 1 , wherein the peptide-polynucleotide complex is about 10 nm to about 150 nm in diameter.
3 . The composition of claim 2 , wherein the peptide-polynucleotide complex is about 10 nm to about 50 nm in diameter.
4 . The composition of claim 1 , wherein the hyaluronic acid coats the peptide-polynucleotide complex.
5 . The composition of claim 1 , wherein the hyaluronic acid is integrated into the peptide-polynucleotide complex.
6 . The composition of claim 1 , wherein the polynucleotide is an RNA.
7 . The composition of claim 1 , wherein the polynucleotide is an mRNA.
8 . The composition of claim 1 , wherein the polynucleotide is a non-coding RNA capable of regulating or inhibiting the expression of a nucleic acid sequence.
9 . The composition of claim 8 , wherein the non-coding RNA is an siRNA.
10 . The composition of claim 8 , wherein the non-coding RNA is an miRNA.
11 . The composition of claim 1 , wherein the peptide is cationic.
12 . The composition of claim 1 , wherein the peptide-polynucleotide complex comprises a ratio of peptide:polynucleotide that is more than about 50:1 and less than about 200:1.
13 . A method of delivering a polynucleotide to the cytoplasm of a cell, the method comprising: contacting a cell with the composition of claim 1 .
14 . A method of treating osteoarthritis in a subject, comprising administering to the subject a therapeutically effective amount of the composition of claim 1 .
Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 7, 2024
From: WASHINGTON UNIVERSITY
To: UNITED STATES DEPARTMENT OF VETERAN AFFAIRS
Reel/Frame 068809/0976 →
LICENSE
Recorded Aug 7, 2024
From: UNIVERSITY OF SOUTH FLORIDA
To: NATIONAL INSTITUTES OF HEALTH
Reel/Frame 068480/0039 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 21, 2023
From: PHAM, CHRISTINE THIEN-NGA; YAN, HUIMIN
To: WASHINGTON UNIVERSITY
Reel/Frame 064006/0229 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 20, 2023
From: WICKLINE, SAMUEL A.; PAN, HUA
To: UNIVERSITY OF SOUTH FLORIDA
Reel/Frame 063998/0891 →
Continuity (3)
Provisional Application
62845974
· May 10, 2019
Related Publication
20230263855A1
· Aug 24, 2023
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