Granted Patent
B2
US 12,305,175 · App. 18/057,860 · Granted May 20, 2025
Modified excisable DAS68416-4 soybean transgenic herbicide resistance locus
Inventors:
Michael Andreas Kock (Rheinfelden, DE); Joshua L. Price (Cambridge, MA); Michael Lee Nuccio (Salem, NH)
Assignee:
INARI AGRICULTURE TECHNOLOGY, INC.
C12N15/8201A01H1/02A01H5/10A01H6/4684A01H6/542C07K14/415C12N9/22C12N15/11C12N15/8213C12N15/8286C12Q1/6834C12Q1/6895C12N2310/20C12N2800/80C12Q2600/13C12Q2600/156C12Q2600/158
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Abstract
Transgenic INHT27 soybean plants comprising modifications of the DAS68416-4 soybean locus which provide for facile excision of the modified DAS68416-4 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.
Claims (14)
1. A transgenic soybean plant cell comprising an INHT27 transgenic locus comprising the DNA molecule set forth in SEQ ID NO: 3.
2. A transgenic soybean plant part comprising the soybean plant cell of claim 1 .
3. The transgenic soybean plant part of claim 2 , wherein said soybean plant part is a seed.
4. A transgenic soybean plant comprising the soybean plant cell of claim 1 .
5. A method for obtaining a bulked population of inbred seed comprising selfing the transgenic soybean plant of claim 4 and harvesting seed comprising the INHT27 transgenic locus from the selfed soybean plant.
6. A method of obtaining hybrid soybean seed comprising crossing the transgenic soybean plant of claim 4 to a second soybean plant which is genetically distinct from the first soybean plant and harvesting seed comprising the INHT27 transgenic locus from the cross.
7. A DNA molecule comprising SEQ ID NO: 3.
8. A processed transgenic soybean plant product comprising the DNA molecule of claim 7 .
9. A biological sample containing the DNA molecule of claim 7 .
10. A method of detecting a soybean plant cell comprising the INHT27 transgenic locus of claim 1 , comprising the step of detecting a DNA molecule comprising SEQ ID NO: 9.
11. A method of excising the INHT 27 transgenic locus from the genome of the soybean plant cell of claim 1 , comprising the steps of:
(a) contacting the INHT 27 transgenic locus of the soybean plant cell with: (i) a Cas 12 a RNA dependent DNA endonuclease (RdDe); and (ii) a guide RNA (gRNA) capable of hybridizing to the guide RNA hybridization site of the originator guide RNA recognition site (OgRRS) and the cognate guide RNA recognition site (CgRRS) of SEQ ID NO: 3; wherein the Cas 12 a RdDe recognizes a OgRRS/gRNA and a CgRRS/gRNA hybridization complex; and,
(b) selecting a transgenic plant cell, transgenic plant part, or transgenic plant wherein the INHT 27 transgenic locus flanked by the OgRRS and the CgRRS has been excised.
12. The method of claim 11 , wherein said guide RNA comprises an RNA sequence encoded by SEQ ID NO: 13.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 15, 2022
From: KOCK, MICHAEL ANDREAS; PRICE, JOSHUA L.; NUCCIO, MICHAEL LEE
To: INARI AGRICULTURE TECHNOLOGY, INC.
Reel/Frame 062100/0551 →
Continuity (13)
Continuation
PCTUS2021043945
· Jul 30, 2021
Provisional Application
63203137
· Jul 9, 2021
Provisional Application
63202569
· Jun 16, 2021
Provisional Application
63201030
· Apr 9, 2021
Provisional Application
63201029
· Apr 9, 2021
Provisional Application
63199951
· Feb 4, 2021
Provisional Application
63199949
· Feb 4, 2021
Provisional Application
63199930
· Feb 3, 2021
Provisional Application
63059860
· Jul 31, 2020
Provisional Application
63059813
· Jul 31, 2020
Provisional Application
63059916
· Jul 31, 2020
Provisional Application
63059963
· Jul 31, 2020
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