Detection of vitamins a and e by tandem mass spectrometry
View Patent ↗Methods are described for measuring the amount of one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample.
1. A method for determining an amount of γ-tocopherol in a sample by tandem mass spectrometry, said method comprising:
a. adding one or more deuterated internal standards to the sample;
b. purifying the sample by turbulent flow liquid chromatography and high pressure liquid chromatography (HPLC);
c. ionizing γ-tocopherol from the purified sample to produce a γ-tocopherol precursor ion and fragmenting the γ-tocopherol precursor ion under a collision cell energy of 34 V to 47 V to generate one or more γ-tocopherol fragment ions detectable by tandem mass spectrometry; and
d. determining an amount of one or more of said γ-tocopherol fragment ions by tandem mass spectrometry;
wherein the amount of the one or more ions determined in step (d) is related to the amount of γ-tocopherol in the sample.
2. The method of claim 1 , wherein said HPLC and said ionization are conducted with on-line processing.
3. The method of claim 1 , wherein said ionization comprises ionization with an atmospheric chemical ionization source.
4. The method of claim 1 , wherein the sample is from a human patient suspected of having a deficiency or an excess of γ-tocopherol.
5. The method of claim 1 , wherein the sample comprises serum or plasma.
6. The method of claim 1 , wherein the tandem mass spectrometry is conducted by multiple reaction monitoring, precursor ion scanning, or product ion scanning.
7. The method of claim 1 , the method further comprising determining an amount of one or more additional analytes simultaneously with the determination of the amount of γ-tocopherol.
8. The method of claim 7 , wherein the one or more additional analytes comprises one or more of x-tocopherol and ß-tocopherol.