IP Library Granted Patent US 12,656,312
Granted Patent B2
US 12,656,312 · App. 18/112,933 · Granted Jun 16, 2026

Biomolecule transfer system and methods

Inventors: Chee Woei Chong (Singapore, SG); Hwee Siong Kuah (Singapore, SG); Mio Xiu Lu Ling (Singapore, SG); Kian Soon Wong (Singapore, SG); Jun Yao Lim (Singapore, SG); Jia Ni Beatrice Lim (Singapore, SG); Li Yong Ong (Singapore, SG); Xin Jie Jeryl Cheng (Singapore, SG); Kok Shyong Chong (Singapore, SG); Zeqi Tan (Singapore, SG); Kguan Tyng Lim (Singapore, SG); Wei Fuh Teo (Singapore, SG); Quoc Cuong Dinh (Singapore, SG); Tong Bao (Singapore, SG); Beng Heng Lim (Singapore, SG); Paul Haney (Rockford, IL); Brian Steer (Carlsbad, CA); Michael Thacker (Carlsbad, CA); Boguslawa Dworecki (Rockford, IL); Kelli Feather-Henigan (Rockford, IL); Xin Mathers (Carlsbad, CA); Shahar Schlezinger (Ness-Ziona, IL); Ronen Benarieh (Ness-Ziona, IL)
Assignees: Life Technologies Corporation; Life Technologies Holdings PTE LTD; Pierce Biotechnology, Inc.; Thermo Fisher Israel LTD
G01N27/44739G01N27/44708
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Quick Facts
Patent No.
US 12,656,312
App. No.
18/112,933
Granted
Jun 16, 2026
Kind
B2
Abstract

A protein transfer system includes at least one base configured to receive one or more consumable protein transfer stacks and at least one lid configured to cover the base. The lid(s) comprise(s) one or more electrodes for supplying current to the one or more consumable protein transfer stacks. The protein transfer system further includes at least one voltage source configured to supply the current to the one or more consumable protein transfer stacks, one or more processors, and one or more hardware storage devices storing instructions that are executable by the one or more processors to configure the protein transfer system to control operation of the one or more voltage sources.

Claims (40)

1 . A protein transfer system, comprising:

at least one base configured to receive one or more consumable protein transfer stacks;

at least one lid engaged with and moveable relative to the at least one base, the at least one lid being configured to cover the at least one base, the at least one lid comprising one or more electrodes for supplying current to the one or more consumable protein transfer stacks,

wherein the at least one lid comprises one or more compression features configured to secure over at least a portion of a perimeter edge of a consumable protein transfer stack of the one or more consumable protein transfer stacks when the at least one lid is closed over the consumable protein transfer stack,

wherein the one or more electrodes comprise one or more cathode plates and one or more anode contacts, and

wherein the one or more cathode plates is/are configured to translate in at least one dimension relative to the one or more compression features;

at least one voltage source comprised in the at least one base and configured to supply the current to the one or more consumable protein transfer stacks;

one or more processors; and

one or more hardware storage devices storing instructions that are executable by the one or more processors to configure the protein transfer system to control operation of the at least one voltage source.

2 . The protein transfer system of claim 1 , further comprising a user interface element configured to display information related to operation of the protein transfer system and/or receive user input for facilitating control of the at least one voltage source.

3 . The protein transfer system of claim 1 , wherein the at least one base comprises two bases.

4 . The protein transfer system of claim 3 , wherein each base of the two bases is independently configured to (1) house a first consumable protein transfer stack of a first size of the one or more consumable protein transfer stacks or (2) house two second consumable protein transfer stacks of the one or more consumable protein transfer stacks, each second consumable protein transfer stack being of a second size, the second size being smaller than the first size.

5 . The protein transfer system of claim 3 , wherein the at least one lid comprises (1) a first lid configured to cover a first base of the two bases, and (2) a second lid configured to cover a second base of the two bases.

6 . The protein transfer system of claim 3 , wherein the protein transfer system is configured to independently control voltage, program method, starting time, ending time, cooling system control, and/or other protein transfer parameters for the two bases.

7 . The protein transfer system of claim 1 , wherein the at least one lid comprises multiple lids, and wherein the at least one voltage source is configured to independently supply voltage to the multiple lids.

8 . The protein transfer system of claim 1 , further comprising a cooling system, the cooling system comprising:

at least one heat sink in thermal communication with a corresponding cathode plate of the one or more cathode plates of a corresponding lid of the at least one lid;

at least one air inlet;

at least one air outlet; and

at least one fan configured to draw air through the at least one air inlet and direct the air along an air-cooling path that extends through the at least one heat sink and toward the at least one air outlet.

9 . The protein transfer system of claim 8 , wherein the at least one air inlet and/or the at least one air outlet is/are arranged on the at least one lid.

10 . The protein transfer system of claim 8 , wherein the instructions are executable by the one or more processors to configure the at least one fan to operate during a protein transfer operation.

11 . The protein transfer system of claim 10 , wherein the instructions are executable by the one or more processors to configure the at least one fan to cause a temperature associated with the at least one base to approach or achieve a predetermined target temperature or temperature curve during the protein transfer operation.

12 . The protein transfer system of claim 8 , wherein the instructions are executable by the one or more processors to configure the at least one fan to automatically begin operating at a predetermined time point during a protein transfer operation.

13 . The protein transfer system of claim 8 , wherein the at least one base comprises a first base and a second base, wherein the cooling system is a first cooling system independently associated with the first base, and wherein the protein transfer system comprises a second cooling system, the second cooling system being independently associated with the second base.

14 . The protein transfer system of claim 1 , wherein the one or more cathode plates are configured to translate from a disengaged position to an engaged position, and vice versa.

15 . A protein transfer system, comprising:

at least one base configured to receive one or more consumable protein transfer stacks;

at least one lid engaged with and moveable relative to the at least one base, the at least one lid being configured to cover the at least one base, the at least one lid comprising one or more electrodes for supplying current to the one or more consumable protein transfer stacks,

at least one voltage source comprised in the at least one base and configured to supply the current to the one or more consumable protein transfer stacks;

one or more processors; and

one or more hardware storage devices storing instructions that are executable by the one or more processors to configure the protein transfer system to control operation of the at least one voltage source,

wherein the instructions are executable by the one or more processors to configure the protein transfer system to perform a consumable detection operation, the consumable detection operation comprising:

closing a consumable detection current path, the consumable detection current path including the one or more electrodes for directing current through the one or more consumable protein transfer stacks, the consumable detection current path further including a dummy load configured to restrict current flow through the consumable detection current path;

applying a consumable detection voltage to the consumable detection current path;

measuring a consumable resistance resulting from the consumable detection voltage being applied to the consumable detection current path;

determining whether the consumable resistance satisfies a consumable detection condition; and

in response to determining that the consumable resistance satisfies the consumable detection condition, opening the consumable detection current path and closing a protein transfer current path, the protein transfer current path including the one or more electrodes for directing current through the one or more consumable protein transfer stacks, the protein transfer current path omitting the dummy load.

16 . The protein transfer system of claim 15 , wherein the instructions are executable by the one or more processors to further configure the protein transfer system to, in response to determining that the consumable resistance satisfies the consumable detection condition, apply a protein transfer voltage to the protein transfer current path to facilitate protein transfer within the one or more consumable protein transfer stacks.

17 . The protein transfer system of claim 16 , wherein the protein transfer voltage is higher than the consumable detection voltage.

Assignments (5)
MERGER Recorded Mar 11, 2026
From: PIERCE BIOTECHNOLOGY, INC.
To: LIFE TECHNOLOGIES CORPORATION
Reel/Frame 075052/0213 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2023
From: STEER, BRIAN; THACKER, MICHAEL; MATHERS, XIN
To: LIFE TECHNOLOGIES CORPORATION
Reel/Frame 062772/0533 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2023
From: CHONG, CHEE WOEI; KUAH, HWEE SIONG; LING, MIO XIU LU; WONG, KIAN SOON; LIM, JUN YAO; LIM, JIA NI BEATRICE; ONG, LI YONG; CHENG, XIN JIE JERYL; CHONG, KOK SHYONG; TAN, ZEQI; LIM, KGUAN TYNG; TEO, WEI FUH; DINH, QUOC CUONG; BAO, TONG; LIM, BENG HENG
To: LIFE TECHNOLOGIES HOLDINGS PTE LTD
Reel/Frame 062772/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2023
From: HANEY, PAUL; DWORECKI, BOGUSLAWA; FEATHER-HENIGAN, KELLI
To: PIERCE BIOTECHNOLOGY, INC.
Reel/Frame 062772/0809 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2023
From: SCHLEZINGER, SHAHAR; BENARIEH, RONEN
To: THERMO FISHER ISRAEL LTD
Reel/Frame 062772/0834 →
Continuity (2)
Provisional Application 63314189 · Feb 25, 2022
Related Publication 20230273153A1 · Aug 31, 2023
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