Instrument for independent electrotransfer in multiple cassettes
Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.
1. An electrotransfer instrument comprising:
a housing with an internal cavity containing a plurality of electroblotting cassettes in a vertical stack;
a power supply;
individual electrical contacts mounted to said internal cavity of said housing for connecting said power supply via the electrical contacts to electrodes in said cassette that is inserted therein; and
electrical relays configured to engage said electrical contacts for each one of said plurality of cassettes upon insertion of said one cassette, and configured to block said power supply to said electrical contacts upon removal of said one cassette.
2. The electrotransfer instrument of claim 1 , further comprising:
a printed circuit board.
3. The electrotransfer instrument of claim 2 , wherein the power supply and printed circuit board are mounted to and within said housing.
4. The electrotransfer instrument of claim 1 , wherein said electrical contacts are spring-loaded contacts.
5. The electrotransfer instrument of claim 1 , wherein said housing comprises a front opening for sliding entry of said electroblotting cassettes.
6. The electrotransfer instrument of claim 1 , wherein said housing comprises magnetic contacts to secure individual electroblotting cassettes within said internal cavity.
7. The electrotransfer instrument of claim 1 , wherein said internal cavity accommodates from two electroblotting cassettes to six electroblotting cassettes.
8. The electrotransfer instrument of claim 1 , wherein said electroblotting cassettes further comprise a gel, a membrane, and buffer-wetted filter papers arranged in a stack.
9. The electrotransfer instrument of claim 8 , wherein said electroblotting cassettes further comprise plate electrodes arranged in a stack, which are connected to said electrical contacts.
10. The electrotransfer instrument of claim 1 , further comprising grooves in internal surfaces of side walls of the cavity, wherein the grooves mate with ridges on sides of the cassette and align the electroblotting cassettes with the electrical contacts.
11. A method for transferring electrophoretically separated species from a slab gel to a sheet-form matrix, said method comprising:
(a) placing said slab gel and a sheet-form matrix in an electroblotting cassette that comprises anode and cathode plates and external electrical contacts for each of said plates;
(b) inserting said cassette in the electrotransfer instrument of claim 1 such that said external electrical contacts are in contact with said individual electrical contacts; and
(c) imposing electrical charges on said electrodes to cause said species to migrate electrophoretically from said slab gel to said sheet-form matrix within said inserted cassette.