Anti-VEGF protein compositions and methods for producing the same
The present disclosure pertains to compositions comprising anti-VEGF proteins.
1. A culture media composition comprising chemically defined media (CDM) and aflibercept, wherein said aflibercept includes about 0.02% to about 1% oxidized aflibercept and wherein said oxidized aflibercept is oxidized at one or more amino acid residues selected from the group consisting of tryptophan, histidine and a combination thereof.
2. The culture media composition of claim 1 , wherein said aflibercept includes between about 0.02% and about 0.5% oxidized aflibercept.
3. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His19 residue oxidation.
4. The culture media composition of claim 3 , wherein a relative abundance of the oxidized His19 residue is at least about 0.02%.
5. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His86 residue oxidation.
6. The culture media composition of claim 5 , wherein a relative abundance of the oxidized His86 residue is at least about 0.02%.
7. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His95 residue oxidation.
8. The culture media composition of claim 7 , wherein a relative abundance of the oxidized His95 residue is at least about 0.03%.
9. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His110 residue oxidation.
10. The culture media composition of claim 9 , wherein a relative abundance of the oxidized His110 residue is at least about 0.04%.
11. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His145 residue oxidation.
12. The culture media composition of claim 11 , wherein a relative abundance of the oxidized His145 residue is at least about 0.19%.
13. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His203 residue oxidation.
14. The culture media composition of claim 1 , wherein said oxidized aflibercept includes His209 residue oxidation.
15. The culture media composition of claim 14 , wherein a relative abundance of the oxidized His209 residue is at least about 0.11%.
16. The culture media composition of claim 1 , wherein said oxidized aflibercept includes Trp58 residue oxidation.
17. The culture media composition of claim 16 , wherein a relative abundance of the oxidized Trp58 residue is at least about 0.03%.
18. The culture media composition of claim 1 , wherein said oxidized aflibercept includes Trp138 residue oxidation.
19. The culture media composition of claim 18 , wherein a relative abundance of the oxidized Trp138 residue is at least about 0.02%.