IP Library › Granted Patent US 12,487,180
Granted Patent B2
US 12,487,180 · App. 18/122,010 · Granted Dec 2, 2025

Device and methods for quantifying analytes introduction

Inventors: Matthew Beaudet (Eugene, OR); Jill Hendrickson (Eugene, OR); David Hagen (Eugene, OR); Rich Meyer (San Mateo, CA)
Assignee: Life Technologies Corporation
G01N21/6486B01L7/52G01N21/6428G01N21/6452B01L3/5082B01L2200/0647B01L2200/143G01N2015/1486G01N2021/6441G01N2035/00782Y10T436/143333
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Quick Facts
Patent No.
US 12,487,180
App. No.
18/122,010
Granted
Dec 2, 2025
Kind
B2
Abstract

Devices and methods for measuring the quantity of multiple analytes in a sample can include a device designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and machine executable instructions configured to select the proper analyte sensing element corresponding to the analyte to be measured.

Claims (22)

1 . A device, comprising:

a sample container configured to contain a sample;

at least a first sensing train and a second sensing train,

the first sensing train comprising a first emission filter and a first photodetector,

the second sensing train comprising a second emission filter and being configured to communicate light received by the second sensing train to a photodetector associated with the second sensing train,

an energy source configured to emit a peak wavelength of light and configured to excite the sample,

the energy source, the first sensing train, and the second sensing train being arranged such that light emanating in a first direction from the sample when excited by the energy source is communicated to the first sensing train and light emanating in a second direction from the sample when excited by the energy source is communicated to the second sensing train, the first direction and the second direction differing from one another.

2 . The device of claim 1 , wherein the energy source is a light emitting diode (LED).

3 . The device of claim 1 , wherein the first emission filter is configured to isolate a first range of wavelengths of light emitted from the sample, and wherein the second emission filter is configured to isolate a second range of wavelengths of light emitted from the sample.

4 . The device of claim 1 , wherein (a) the first emission filter filters out light with a wavelength of less than about 520 nm and greater than about 580 nm, and/or (b) wherein the second emission filter filters out light with a wavelength of less than about 652 nm.

5 . The device of claim 1 , wherein first photodetector comprises a photomultiplier, a phototube, a photoresistor, a photovoltaic cell, a photodiode, a phototransistor, or a pyroelectric device that detects changes in temperature due to illumination.

6 . The device of claim 1 , comprising:

a user interface configured to receive a user selection of analyte; and

a computer processing unit with machine executable instructions configured to select or make adjustments to a sensing train corresponding to the analyte.

7 . The device of claim 1 , wherein the photodetector associated with the second sensing train is the first photodetector.

8 . The device of claim 6 , wherein the analyte is a nucleic acid, a double stranded DNA, a single stranded DNA, a RNA, a microRNA, a protein, a cell, a virus, a carbohydrate, a lipids, a dye, a metal ion or a combination thereof.

9 . The device of claim 6 , wherein the machine executable instructions are capable of selecting the analyte without user input.

10 . The device of claim 6 , where the machine executable instructions further comprise calibration data including calibration curve data, internal standard data, blank measurement data, standard measurement data, and combinations thereof.

11 . The device of claim 1 , further comprising a second photodetector and wherein the photodetector associated with the second sensing train is the second photodetector.

12 . The device of claim 1 , wherein the sample container is a tube, a petri dish, a culture flask, a multiwell plate, or a cuvette.

13 . The device of claim 6 , wherein each of the first and second sensing trains is configured to measure a presence and/or a quantity of the selected analyte, wherein the analyte is a cell, a virus, a nucleic acid, a DNA, a dsDNA, a ssDNA, a RNA, a mRNA, a microRNA, a protein, a carbohydrate, a lipids, a dye, a metal ion or a combination thereof.

14 . The device of claim 1 , wherein light emanating in a first direction from the sample when excited by the energy source is communicated to the first sensing train along a first pathway, wherein light emanating in a second direction from the sample when excited by the energy source is communicated to the second sensing train along a second pathway, the first pathway and the second pathway differing from one another.

Assignments (2)
CHANGE OF NAME Recorded Mar 15, 2023
From: INVITROGEN CORPORATION
To: LIFE TECHNOLOGIES CORPORATION
Reel/Frame 062994/0946 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 15, 2023
From: BEAUDET, MATTHEW P.; HAGEN, DAVID C.; HENDRICKSON, JILL; MEYER, RICH B.
To: INVITROGEN CORPORATION
Reel/Frame 063101/0814 →
Continuity (9)
Continuation 17211154 · Mar 24, 2021
Continuation 16735856 · Jan 7, 2020
Continuation 15922477 · Mar 15, 2018
Division 14851046 · Sep 11, 2015
Continuation 14017965 · Sep 4, 2013
Continuation 11626842 · Jan 24, 2007
Provisional Application 60862422 · Oct 20, 2006
Provisional Application 60762008 · Jan 24, 2006
Related Publication 20230384227A1 · Nov 30, 2023
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