Apparatus for holding a microfluidic chip
A microfluidic chip configuration wherein injection occurs in an upwards vertical direction, and fluid vessels are located below the chip in order to minimize particle settling before and at the analysis portion of the chip's channels. The input and fluid flow up through the bottom of the chip, in one aspect using a manifold, which avoids orthogonal re-orientation of fluid dynamics. The contents of the vial are located below the chip and pumped upwards and vertically directly into the first channel of the chip. A long channel extends from the bottom of the chip to near the top of the chip. Then the channel takes a short horizontal turn that nearly negates any influence of cell settling due to gravity and zero flow velocity at the walls. The fluid is pumped up to a horizontal analysis portion that is the highest channel/fluidic point in the chip and thus close to the top of the chip, which results in clearer imaging. A laser may also suspend cells or particles in this channel during analysis which prevents them from settling.
1 . A system for analyzing and imaging particles or cells in fluids, comprising:
a microfluidic chip, comprising:
a substrate comprising a plurality of channels configured to transport one or more substances, wherein the plurality of channels comprises:
a first channel oriented vertically upwards with respect to gravity within the substrate,
a second channel in operable communication with the first channel and disposed horizontally within the substrate,
a third channel in operable communication with the second channel and oriented vertically upwards with respect to gravity within the substrate, and
a fourth channel in operable communication with the third channel and disposed horizontally within the substrate;
a light source configured to illuminate at least one channel of the plurality of channels; and
an apparatus for holding the microfluidic chip, comprising:
a cavity configured to hold the microfluidic chip vertically with respect to gravity;
an integrated light source holder configured to allow the light source to focus illumination into the at least one channel of the plurality of channels;
an integrated prism holder disposed between the integrated light source holder and the cavity to position a prism to reflect light from the light source in the integrated light source holder to a desired location in or on the microfluidic chip;
threaded screw holes for screws to hold and allow for adjustment of the microfluidic chip; and
threaded or non-threaded pass through holes positioned at the bottom of the apparatus for tubing and connector(s).
2 . The system of claim 1 , wherein the light source comprises at least one of a light emitting diode (LED), organic light emitting diode (OLED), fiber optic, fiber bundle, laser, flash tube, fluorescent lamp, incandescent lamp, and/or halogen lamp.
3 . The system of claim 1 , wherein the integrated prism holder comprises a prism received therein.
4 . The system of claim 3 , wherein the prism is positioned to direct light through a bottom surface of the microfluidic chip to the fourth channel.
5 . The system of claim 4 , wherein the fourth channel is disposed within the top 50% of the microfluidic chip.
6 . The system of claim 4 , wherein the fourth channel is disposed within 100 microns to 2 millimeters from the top of the microfluidic chip.
7 . The system of claim 1 , wherein light from the light source is guided by an integrated channel within the integrated light source holder to the integrated prism holder or to the desired location on the microfluidic chip.
8 . The system of claim 7 , wherein the integrated channel connects the integrated light source holder to the integrated prism holder.
9 . The system of claim 1 , further comprising adjustable screws integrated into the threaded screw holes.
10 . The system of claim 1 , wherein the light reflected to the desired location in or on the microfluidic chip is conical.
11 . The system of claim 1 , wherein the desired location in or on the microfluidic chip is at least one channel of the plurality of channels in the microfluidic chip or a portion thereof.
12 . The system of claim 1 , wherein a direction between the integrated light source holder and the integrated prism holder is orthogonal to a direction between the prism holder and the cavity.
13 . The system of claim 1 , further comprising an opening through which a sample can be injected through a bottom surface of the microfluidic chip.