Paired laser and electrokinetic separation, manipulation, and analysis device
The combined value of integrating optical forces and electrokinetics allows for the pooled separation vectors of each to be applied, providing for separation based on combinations of features such as size, shape, refractive index, charge, charge distribution, charge mobility, permittivity, and deformability. The interplay of these separation vectors allow for the selective manipulation of analytes with a finer degree of variation. Embodiments include methods of method of separating particles in a microfluidic channel using a device comprising a microfluidic channel, a source of laser light focused by an optic into the microfluidic channel, and a source of electrical field operationally connected to the microfluidic channel via electrodes so that the laser light and the electrical field to act jointly on the particles in the microfluidic channel. Other devices and methods are disclosed.
1. A device comprising:
a microfluidic channel configured to supply a linear or non-linear dielectrophoretic (DEP) field to an interior of the channel via a (1) DEP electrode system or (2) insulator DEP system, the channel having a region of shaped wall geometry or obstruction geometry configured to create at least one restriction in the channel, and
a source of laser light focused by an optic into the microfluidic channel,
wherein the laser light and DEP field operate jointly on particles in the microfluidic channel to trap the particles or modify their velocity,
wherein the DEP field exists across a length of the channel and is generated by electrodes positioned beyond the region of shaped wall geometry or obstruction geometry at inlet and outlet ends of the channel.
2. A device comprising:
a microfluidic channel configured to supply a linear or non-linear dielectrophoretic (DEP) field to an interior of the channel via a (1) DEP electrode system or (2) insulator DEP system, the channel having a region of shaped wall geometry or obstruction geometry configured to create at least one restriction in the channel, and
a source of laser light focused by an optic into the microfluidic channel,
wherein the laser light and DEP field operate jointly on particles in the microfluidic channel to trap the particles or modify their velocity,
wherein the DEP field exists across a length of the channel and is generated by electrodes positioned beyond the region of shaped wall geometry or obstruction geometry at inlet and outlet ends of the channel; and
an adjoining channel structure, wherein said DEP field is effective to operate on populations of particles based on their electrokinetic properties, and said laser light is focused orthogonally to a flow axis of said microfluidic channel and operates to elute one or more of the isolated populations into the adjoining channel structure,
wherein the adjoining channel structure is a region open to the channel at only a single end.
3. The device of claim 1 , wherein the channel has said curved or triangular shaped wall geometry or round obstruction geometry.
4. A device comprising:
a microfluidic channel configured to supply a linear or non-linear dielectrophoretic (DEP) field to an interior of the channel via a (1) DEP electrode system or (2) insulator DEP system, the channel having a region of shaped wall geometry or obstruction geometry configured to create at least one restriction in the channel, and
a source of laser light focused by an optic into the microfluidic channel,
wherein the laser light and DEP field operate jointly on particles in the microfluidic channel to trap the particles or modify their velocity,
wherein the DEP field exists across a length of the channel and is generated by electrodes positioned beyond the region of shaped wall geometry or obstruction geometry at inlet and outlet ends of the channel , and
wherein said laser light is configured to be adjustably angled via mirrors and focusing optics held in place with optomechanical components and configured with the necessary degrees of freedom to direct and align the laser into the microfluidic channel.