IP Library › Granted Patent US 12,234,512
Granted Patent B2
US 12,234,512 · App. 18/164,439 · Granted Feb 25, 2025

Compositions and methods for detecting rare sequence variants

Inventors: Shengrong Lin (Fremont, CA); Zhaohui Sun (Albany, CA); Grace Qizhi Zhao (Palo Alto, CA); Paul Ling-Fung Tang (San Francisco, CA)
Assignee: AccuraGen Holdings Limited
C12Q1/6874C12Q1/6827C12Q1/6869
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Quick Facts
Patent No.
US 12,234,512
App. No.
18/164,439
Granted
Feb 25, 2025
Kind
B2
Abstract

In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods.

Claims (25)

1. A method of distinguishing a sequence variant from an error in a cell-free deoxyribonucleic acid (DNA) polynucleotide, comprising:

(a) circularizing said cell-free DNA polynucleotide or a derivative thereof to form a circular DNA polynucleotide, wherein said cell-free DNA polynucleotide is not enzymatically phosphorylated prior to circularization, and wherein said cell-free DNA polynucleotide is single-stranded;

(b) amplifying said circular DNA polynucleotide of (a) to produce a plurality of concatemers, wherein a concatemer of said plurality of concatemers comprises at least two copies of a sequence of said circular DNA polynucleotide;

(c) sequencing said plurality of concatemers to produce a plurality of sequencing reads; and

(d) identifying a sequence difference between a sequencing read of said plurality of sequencing reads and a reference sequence as said sequence variant only when said sequence difference occurs in at least two copies of said circular DNA polynucleotide in said concatemer and not when said sequence difference occurs in only one copy of said circular DNA polynucleotide in said concatemer, thereby distinguishing said sequence variant from said error.

2. The method of claim 1 , wherein circularizing is effected by subjecting said cell-free DNA polynucleotide to a ligation reaction using a ligase.

3. The method of claim 2 , wherein ligation is effected by a ligase, and said method further comprises degrading said ligase prior to said amplifying of (b).

4. The method of claim 1 , wherein said sequence variant is a single nucleotide polymorphism.

5. The method of claim 1 , wherein said reference sequence is a consensus sequence formed by aligning a plurality of sequencing reads with one another.

6. The method of claim 1 , wherein said reference sequence is a known reference sequence.

7. The method of claim 1 , wherein (b) comprises performing a primer extension reaction selected from the group consisting of: reverse transcription, polymerase chain reaction, isothermal amplification, and combinations thereof.

8. The method of claim 1 , wherein said amplifying is effected by using a polymerase having strand-displacement activity.

9. The method of claim 1 , wherein said amplifying comprises subjecting said circular DNA polynucleotide to an amplification reaction mixture comprising random primers.

10. The method of claim 1 , wherein said amplifying comprises subjecting said circular DNA polynucleotide to an amplification reaction mixture comprising one or more primers, each of which specifically hybridizes to a different target sequence via sequence complementarity.

11. The method of claim 1 , wherein said concatemers are subjected to said sequencing step without enrichment.

12. The method of claim 1 , further comprising performing an enrichment on said concatemers or derivatives thereof to enrich one or more target polynucleotides.

13. The method of claim 12 , wherein said enrichment comprises amplifying a target sequence comprising sequence A and sequence B oriented in a 5′ to 3′ direction in an amplification reaction mixture, said reaction mixture comprising:

(a) said concatemers;

(b) a first primer comprising sequence A′, wherein said first primer specifically hybridizes to sequence A of said target sequence via sequence complementarity between sequence A and sequence A′;

(c) a second primer comprising sequence B, wherein said second primer specifically hybridizes to sequence B′ present in a complementary polynucleotide comprising a complement of said target sequence via sequence complementarity between B and B′; and

(d) a polymerase that extends said first primer and said second primer to produce amplified polynucleotides;

wherein the distance between a 5′ end of sequence A and a 3′ end of sequence B of said target sequence is 75 nucleotides (nt) or less; and

wherein sequence A and sequence B′ are present in said cell-free DNA polynucleotide.

14. The method of claim 1 , wherein said cell-free DNA polynucleotide comprises cell-free tumor DNA.

15. The method of claim 1 , wherein identifying said sequence difference is performed by a computer system based on said sequencing reads and said reference sequence.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 23, 2025
From: ACCURAGEN HOLDINGS LIMITED
To: ACCUSCAN SCIENCES INC.
Reel/Frame 074035/0680 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 9, 2023
From: LIN, SHENGRONG; SUN, ZHAOHUI; ZHAO, GRACE QIZHI; TANG, PAUL LING-FUNG
To: ACCURAGEN, INC.
Reel/Frame 063914/0163 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 9, 2023
From: ACCURAGEN, INC.
To: ACCURAGEN HOLDINGS LIMITED
Reel/Frame 063914/0209 →
Continuity (6)
Continuation 16945553 · Jul 31, 2020
Continuation 15102241
Provisional Application 62010975 · Jun 11, 2014
Provisional Application 61987414 · May 1, 2014
Provisional Application 61914907 · Dec 11, 2013
Related Publication 20230416819A1 · Dec 28, 2023
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