Granted Patent
B2
US 12,421,532 · App. 18/171,272 · Granted Sep 23, 2025
Polynucleotides encoding engineered imine reductases
Inventors:
Haibin Chen (Beijing, CN); Steven J. Collier (Concord, MA); Jovana Nazor (Milpitas, CA); Joly Sukumaran (Singapore, SG); Derek Smith (Singapore, SG); Jeffrey C. Moore (Westfield, NJ); Gregory Hughes (Scotch Plains, NJ); Jacob Janey (New York, NY); Gjalt W. Huisman (Redwood City, CA); Scott J. Novick (Palo Alto, CA); Nicholas J. Agard (San Francisco, CA); Oscar Alvizo (Fremont, CA); Gregory A. Cope (Menlo Park, CA); Wan Lin Yeo (Singapore, SG); Stefanie Ng Minor (Redwood City, CA)
Assignee:
Codexis, Inc.
C12P13/02C12N9/0028C12P13/001C12P13/04C12P13/06C12P17/10C12P17/12C12P17/165C12P17/185C12P17/188C12Y105/01C12Y105/01023C12Y105/01024C12Y105/01028Y02P20/52
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Abstract
The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Claims (6)
1. An engineered polynucleotide encoding an engineered polypeptide having imine reductase activity, wherein said polypeptide has at least 93% sequence identity to the polypeptide of SEQ ID NO: 2, and comprises a substitution at a position corresponding to position 111, 136, 156, 259, or 292 of the polypeptide of SEQ ID NO:2.
2. A vector comprising the engineered polynucleotide of claim 1 .
3. The vector of claim 2 , further comprising at least one control sequence.
4. A host cell comprising the vector of claim 2 .
5. A host cell comprising the vector of claim 3 .
6. The engineered polynucleotide of claim 1 , wherein the amino acid at the position corresponding to position 136 of the polypeptide of SEQ ID NO:2 has been replaced with a glycine.
Assignments (1)
SECURITY INTEREST
Recorded Feb 15, 2024
From: CODEXIS, INC.
To: INNOVATUS LIFE SCIENCES LENDING FUND I, LP, AS COLLATERAL AGENT
Reel/Frame 066600/0650 →
Continuity (17)
Continuation
15710462
· Sep 20, 2017
Provisional Application
61646100
· May 11, 2012
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