Apparatus and method for obtaining protein-enriched fractions from breast milk

1. A vertically integrated device for on-site obtaining protein-enriched fractions from human or animal breast milk, comprising:

a first reservoir having a volume of 1.5 L or less;

a delipidating unit for reducing a lipid content in the human or animal breast milk to obtain delipidated milk, wherein the delipidating unit comprises a single-usage first filter, wherein the first reservoir is connected to the single-usage first filter, wherein a valve is arranged in a flow line between the first reservoir and the single-usage first filter;

a second reservoir having a volume of 0.5 L, configured to receive an output of the single-usage first filter;

an air pressure system, connected to the second reservoir in order to support a flow of the human or animal breast milk through the first filter passing a second valve;

a third reservoir having a volume of 0.5 L, connected to the second reservoir;

a filtering unit for increasing a protein concentration of the delipidated milk to obtain the protein-enriched fraction, comprising a single-usage second filter having a membrane and having a nominal molecular weight limit of 2 kDa or more, wherein the single-usage second filter is arranged in a passage between the second reservoir and the third reservoir, and wherein a further connection connects the air pressure system to the passage passing a third valve to support the extraction of filtrate collected in the third reservoir from an intermediate product stored in the second reservoir; and

a stirring device configured to prevent accumulation of proteins on a surface of the membrane of the single-usage second filter;

wherein

(i) the delipidating unit and the filtering unit are vertically integrated; and

(ii) the device is configured for on-site application.

2. The device of claim 1 , wherein the single-usage first filter of the delipidating unit is a glass microfiber filter.

3. The device of claim 1 , wherein the single-usage first filter of the delipidating unit has a pore size of less than 0.5 μm.

4. The device of claim 3 , wherein the single-usage first filter of the delipidating unit has a pore size of less than 0.35 μm.

5. The device of claim 1 , wherein the delipidating unit and the filtering unit are arranged in a closed system wherein the delipidated milk is transferred from the delipidating unit to the filtering unit.

6. The device of claim 1 , wherein the single-usage second filter of the filtering unit has a nominal molecular weight limit of 5 kDa or more.

7. A device for obtaining protein-enriched fractions from human or animal breast milk, comprising:

a first reservoir having a volume of 1.5 L or less;

a delipidating unit comprising a single-usage first filter, wherein the first reservoir is connected to the single-usage first filter, wherein a first valve is arranged in a flow line between the first reservoir and the single-usage first filter;

a second reservoir having a volume of 0.5 L and comprising a lid configured to receive the output of the first filter, wherein the second reservoir further comprises a stirring device;

a vacuum source connected through a second valve to the lid of the second reservoir; and

a filtering unit comprising a single-usage second filter and a third reservoir, wherein

(i) the single-usage second filter is arranged in a passage between the second reservoir and the third reservoir,

(ii) the vacuum source is connected through a third valve to the passage; and

(iii) the third reservoir has a volume of 0.5 L;

wherein the delipidating unit, the second reservoir, the third reservoir, and the filtering unit are vertically integrated in a closed system; and

wherein the device is configured for on-site application.

8. The device of claim 7 , wherein the single-usage first filter of the delipidating unit is a glass microfiber filter.

9. The device of claim 7 , wherein the single-usage first filter of the delipidating unit has a pore size of less than 0.5 μm.

10. The device of claim 9 , wherein the single-usage first filter of the delipidating unit has a pore size of less than 0.35 μm.

11. The device of claim 7 , wherein the single-usage second filter of the filtering unit has a nominal molecular weight limit of 5 kDa or more.