Promoter polynucleotide, signal polypeptide and use thereof
Provided are a promoter polynucleotide, a signal polypeptide and a polynucleotide encoding the signal polypeptide, and use thereof. A vector and a host cell each including the promoter polynucleotide and the polynucleotide encoding the signal polypeptide may efficiently express and/or extracellularly secrete a foreign protein.
1. A polypeptide comprising from the N-terminus, a first polypeptide of isolated signal polypeptide having the amino acid sequence of SEQ ID NO:2, and a second polypeptide of a protein, which is heterologous to the isolated signal polypeptide.
2. A recombinant polynucleotide comprising a polynucleotide encoding the polypeptide of claim 1 , which comprises a polynucleotide encoding the first polypeptide and a polynucleotide encoding the second polypeptide.
3. The recombinant polynucleotide of claim 2 , wherein the polynucleotide encoding the isolated signal polypeptide is operatively linked to the polynucleotide encoding the second polypeptide.
4. The recombinant polynucleotide of claim 3 , further comprising a promoter, wherein the promoter is operably linked to the polynucleotide encoding the first polypeptide.
5. The recombinant polynucleotide of claim 2 , further comprising a promoter, wherein the promoter is operably linked to the polynucleotide encoding the first polypeptide.
6. The recombinant polynucleotide of claim 2 , which is a vector.
7. A host cell comprising the recombinant polynucleotide of claim 2 .
8. A method of producing a protein, comprising culturing the host cell of claim 7 in a medium; and isolating the protein from the culture.
9. The recombinant polynucleotide of claim 3 , which is a vector.
10. The recombinant polynucleotide of claim 4 , which is a vector.
11. The recombinant polynucleotide of claim 5 , which is a vector.
12. A host cell comprising the recombinant polynucleotide of claim 3 .
13. A host cell comprising the recombinant polynucleotide of claim 4 .
14. A host cell comprising the recombinant polynucleotide of claim 5 .
15. A method of producing a protein, comprising culturing the host cell of claim 12 in a medium; and isolating the protein from the culture.
16. A method of producing a protein, comprising culturing the host cell of claim 13 in a medium; and isolating the protein from the culture.
17. A method of producing a protein, comprising culturing the host cell of claim 14 in a medium; and isolating the protein from the culture.