IP Library › Granted Patent US 12,313,588
Granted Patent B2
US 12,313,588 · App. 18/316,920 · Granted May 27, 2025

Molded flow channel

Inventors: Edward Liu (Saratoga, CA); Kenneth M. Stothers (San Jose, CA); Markus Wallgren (Los Altos Hills, CA); Janusz B. Wojtowicz (Sunnyvale, CA); Robert A. Yuan (San Jose, CA)
Assignee: Roche Sequencing Solutions, Inc.
G01N27/44791G01N33/48721
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Quick Facts
Patent No.
US 12,313,588
App. No.
18/316,920
Granted
May 27, 2025
Kind
B2
Abstract

A nanopore based sequencing system includes a plurality of nanopore sensors. Each nanopore sensor has a portion for receiving a fluid. The nanopore based sequencing system includes a fluid chamber configured to guide the fluid over the plurality of nanopore sensors and an inlet configured to deliver the fluid into the fluid chamber. At least a portion of the fluid chamber is made of a material that has been molded around at least a portion of an electrode.

Claims (12)

1. A nanopore based sequencing system, comprising:

a nanopore sensor array comprising a plurality of nanopore sensors, each nanopore sensor comprising a working electrode;

a counter electrode insert;

a molded flow channel configured to be disposed over the nanopore sensor array, the molded flow channel comprising at least one fluid guide and a molded portion that has been molded around at least a portion of the counter electrode insert;

wherein the molded flow channel is configured to form a fluid chamber over the nanopore sensor array when the molded flow channel is disposed over the nanopore sensor array, wherein the at least one fluid guide is configured to form a fluid path within fluid chamber; and

an inlet configured to deliver the fluid into the fluid chamber.

2. The nanopore based sequencing system of claim 1 , further comprising an outlet configured to deliver the fluid out of the fluid chamber.

3. The nanopore based sequencing system of claim 1 , wherein the fluid path is linear.

4. The nanopore based sequencing system of claim 1 , wherein the fluid path is serpentine.

5. The nanopore based sequencing system of claim 1 , wherein the molded portion is formed by injection molding.

6. The nanopore based sequencing system of claim 1 , wherein the molded portion is formed by transfer molding.

7. The nanopore based sequencing system of claim 1 , wherein the molded portion is formed by compression molding.

Continuity (6)
Continuation 16947043 · Jul 15, 2020
Continuation 15410586 · Jan 19, 2017
Provisional Application 62286826 · Jan 25, 2016
Provisional Application 62281662 · Jan 21, 2016
Provisional Application 62281663 · Jan 21, 2016
Related Publication 20230280308A1 · Sep 7, 2023
References Cited (6)
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US 10234446B2 · Yuan · 2019 [cited by applicant]
US 20130252234A1 · Nassef · 2013 [cited by applicant]
US 20160290988A1 · Yokoi et al. · 2016 [cited by applicant]
WO 2008124706A2 · 2008 [cited by applicant]
Chen, X., DNA sequencing with titanium nitride electrodes, Quantum Chemistry, vol. 113 No. 20, pp. 2295-2305, (2013). [cited by applicant]