Methane monooxygenase enzymes
Improved soluble methane monooxygenases and soluble methane monooxygenase systems are provided.
1. A mutant soluble diiron monooxygenase system, comprising a mutant hydroxylase gamma subunit comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 5, wherein the mutant hydroxylase gamma subunit comprises a mutation at position 70, wherein the residue position is numbered with respect to SEQ ID NO: 5.
2. The mutant soluble diiron monooxygenase hydroxylase gamma subunit of claim 1 , wherein the mutation comprises a substitution at residue position R70E, wherein the residue position is numbered with respect to SEQ ID NO: 5.
3. One or more isolated nucleic acids encoding one or more polypeptides comprising the mutant soluble diiron monooxygenase system of claim 1 .
4. One or more vectors comprising the isolated nucleic acids of claim 3 .
5. One or more host cells comprising or consisting of the one or more vectors of claim 4 .
6. The one or more host cells of claim 5 , wherein the one or more host cells comprise a prokaryotic cell.
7. The one or more host cells of claim 6 , wherein the prokaryotic cell comprises one or more of Escherichia coli, Corynebacterium glutamicum , and/or Bacillus methanolicus.