Systems and methods for bioprocessing
A bioprocessing system includes a first module configured for enriching and isolating a population of cells, a second module configured for activating, genetically modifying, and expanding the population of cells, and a third module configured for harvesting the expanded population of cells.
1 . A bioprocessing system, comprising: a first module configured for enriching and isolating a first population of cells; a second module configured for activating, genetically modifying, and expanding the first population of cells; and a third module configured for harvesting the expanded first population of cells; wherein the first module is configured for enriching a second population of cells while the second module is carrying out activation, genetic modification, and/or expansion on the first population of cells.
2 . The bioprocessing system of claim 1 , wherein: the second module contains a bioreactor vessel configured to provide for the genetic modification and expansion of the population of cells without removal of the population of cells from the bioreactor vessel, or the second module contains a bioreactor vessel configured to provide for the activation, genetic modification and expansion of the population of cells without removal of the population of cells from the bioreactor vessel.
3 . The bioprocessing system of claim 1 , wherein: the second module includes a first bioreactor vessel and a second bioreactor vessel fluidly interconnected with the first bioreactor vessel.
4 . The bioprocessing system of claim 3 , wherein: the first bioreactor vessel is configured for carrying out activation and genetic modification of the population of cells; wherein the second bioreactor vessel is configured for carrying out expansion of the population of cells.
5 . The bioprocessing system of claim 4 , wherein the bioprocessing system is configured to effect activation and genetic modification of the cells in the first bioreactor vessel, transfer of the cells from the first bioreactor vessel to the second bioreactor vessel, and expansion of the cells in the second bioreactor vessel in an autonomous manner.
6 . The bioprocessing system of claim 3 , wherein: the first bioreactor vessel is configured for performing activation of the population of cells; and the second bioreactor vessel is configured for performing genetic modification and expansion of the population of cells.
7 . The bioprocessing system of claim 1 , wherein at least one of the first module, second module, and/or third module includes a bioreactor vessel configured for filterless perfusion.
8 . The bioprocessing system of claim 1 , wherein: the population of cells is a first population of cells; and wherein the bioprocessing system is configured to support activation, genetic modification and expansion of the first population of cells in the second module simultaneously with enrichment and isolation of a second population of cells in the first module, wherein the first population of cells is different from the second population of cells.
9 . The bioprocessing system of claim 1 , further comprising: a plurality of second modules, each second module configured for activating, genetically modifying and expanding the cells; wherein each second module is configured to support activation, genetic modification and expansion of different populations of cells in parallel.
10 . The bioprocessing system of claim 9 , wherein: the first module is configured for enrichment and isolation of each different population of cells prior to transfer to one of the plurality of second modules for activation, genetic modification and expansion.
11 . The bioprocessing system of claim 10 , wherein: the bioprocessing system is configured to perform enrichment and isolation of one of the different populations of cells in the first module simultaneously with activation, genetic modification and expansion of another of the different populations of cells in the second module.
12 . A bioprocessing system, comprising: a first module configured for enriching and isolating a population of cells; at least two second modules configured for activating, genetically modifying, and expanding an isolated population of cells; a third module configured for harvesting the expanded population of cells; and wherein the first module is configured for enriching a second population of cells while the second module is carrying out activation, genetic modification, and/or expansion on a first population of cells; and wherein after the first module transfers a first population of isolated cells to a first second module of the at least two second modules, the first module prepares and provides for transfer of the second population of isolated cells to a second module while the first second module is activating, genetically modifying, and/or expanding the first population of isolated cells.
13 . The bioprocessing system of claim 12 , further comprising:
wherein the third module is configured for enriching an isolating a population of cells and provides an additional population of isolated cells to at least one additional second module during the operation of at least one of the at least two second modules.
14 . The bioprocessing system of claim 1 , wherein each module further comprises a sterile, closed fluid path with at least one input port and at least one output port, such that the first, second, and third modules are fluidically isolated from each other.
15 . The bioprocessing system of claim 1 , wherein the system is configured for parallel and asynchronous processing of multiple populations of cells.
16 . The bioprocessing system of claim 1 , further comprising an additional second module, wherein the additional second module is configured for activating, genetically modifying, and expanding the second population of cells while the second module is activating, genetically modifying, and expanding the first population of cells.