Dry powder formulation of caveolin-1 peptides and methods of use thereof
Provided herein are compositions comprising caveolin-1 (Cav-1) peptides. Further provided are methods of using the Cav-1 peptides for the treatment of lung infections or acute or chronic lung injury, particularly lung fibrosis.
1. A method of treating a subject having chronic obstructive pulmonary disease (COPD), the method comprising administering to the subject a dry powder formulation comprising a peptide, or a pharmaceutically acceptable salt thereof, and wherein the dry powder formulation does not comprise a pharmaceutically acceptable carrier or excipient;
wherein the peptide's amino acid sequence is FTTFTVT (SEQ ID NO: 2) or the peptide's amino acid sequence has 1-5 amino acids additional sequence at the N- and/or C-terminus of SEQ ID NO: 2; and wherein administering to the subject is by a dry powder inhaler.
2. The method of claim 1 , wherein about 1 mg to about 10 mg of the peptide is administered to the subject.
3. The method of claim 1 , wherein the peptide is administered to the subject at about 0.001 mg/kg to about 0.1 mg/kg based on the subject's weight.
4. A method of treating a subject in need thereof, the method comprising administering to the subject an effective amount of a dry powder composition comprising particles,
wherein the particles comprise a peptide consisting of FTTFTVT (SEQ ID NO: 2) or a pharmaceutically acceptable salt thereof,
wherein the particles have a median particle diameter of less than 5 μm,
wherein the dry powder composition does not comprise a pharmaceutically acceptable carrier or excipient; and
wherein the dry powder composition is administered to the subject using a dry powder inhaler.
5. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising a FPF of at least 50% or at least 60% of an emitted dose.
6. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising a FPF of at least 70% of an emitted dose.
7. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising a FPF of at least 80% of an emitted dose.
8. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising an emitted fraction (EF) of at least 80%.
9. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising an EF of at least 90%.
10. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising a median mass aerodynamic diameter (MMAD) of less than 5 μm.
11. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising a MMAD of about 1.75 μm to about 3 μm.
12. The method of claim 4 , wherein the dry powder inhaler provides an aerosol comprising a MMAD of about 1.75 μm.
13. The method of claim 4 , wherein the dry powder composition is administered in one unit dose.
14. The method of claim 4 , wherein the dry powder composition is administered in more than one unit dose.
15. The method of claim 4 , wherein an emitted dose to the subject is at least 10 mg, at least 15 mg, or at least 20 mg.
16. The method of claim 4 , wherein an emitted dose to the subject is at least 20 mg.
17. The method of claim 4 , wherein a fine particle dose to the subject is greater than 5 mg.
18. The method of claim 4 , wherein the subject has an inflammatory disorder.
19. The method of claim 4 , wherein the subject has a fibrotic condition.
20. The method of claim 4 , wherein the subject has pulmonary inflammation, acute lung injury, a lung infection, COPD, chemical-induced lung injury, plastic bronchitis, asthma, acute respiratory distress syndrome (ARDS), inhalational smoke-induced acute lung injury (ISALI), bronchiolitis, bronchiolitis obliterans, a fibrotic condition of the lungs, interstitial lung disease, idiopathic pulmonary fibrosis, or lung scarring.
21. The method of claim 4 , wherein the subject has interstitial lung disease.
22. The method of claim 4 , wherein the subject has idiopathic pulmonary fibrosis.
23. The method of claim 4 , wherein the method comprises administering at least one additional therapeutic to the subject.
24. The method of claim 23 , wherein the at least one additional therapeutic is a non-steroidal anti-inflammatory drug (NSAID), a steroid, a disease-modifying antirheumatic drug (DMARD), an immunosuppressive, or a bronchodilator.
25. The method of claim 4 , wherein the pharmaceutically acceptable salt is an ammonium salt or an acetate salt.
26. The method of claim 4 , wherein the dry powder composition comprises particles consisting of a peptide consisting of FTTFTVT (SEQ ID NO: 2) or a pharmaceutically acceptable salt thereof, and wherein the dry powder composition is at least about 95% (w/v) pure.
27. The method of claim 4 , wherein the particles have a median particle diameter between about 1 μm to about 5 μm.
28. The method of claim 4 , wherein the particles have a median particle diameter of about 2 μm, about 2.5 μm, or about 3 μm.
29. The method of claim 4 , wherein the dry powder composition is stable for at least 1 month, at least 3 months, or at least 6 months.