Composition and method for generating immunity to borrelia burgdorferi
Provided is an immunogenic composition including a peptide, wherein consecutive amino acids of the peptide include at least amino acids of SEQ ID NO:1 and one or more adjuvants. Also provided is a method of vaccinating a subject against Borrelia burgdorferi , including administering to the subject an effective amount of the immunogenic composition.
1. An immunogenic composition, comprising
a peptide, comprising at least any eight consecutive amino acids from amino acids 186-196 of SEQ ID NO: 1, except that each amino acid corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine or is independently substituted with any other amino acid except tyrosine, and
an adjuvant selected from the group consisting of an aluminum salt, AS04, AS03, monophosphoryl lipid A, poly(I:C), a CpG DNA adjuvant, MF59, an emulsion adjuvant comprising squalene and water, a combination adjuvant comprising block copolymer CRL-8300, squalene, a sorbitan monooleateor, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium salt (DOTAP), 3 β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-chol liposome), and a virosomal adjuvant.
2. The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1, and from 0 to 2 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid.
3. The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid.
4. The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 197-222 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-222 of SEQ ID NO: 1 is independently substituted with any other amino acid.
5. The immunogenic composition of claim 1 , wherein the peptide comprises amino acids 1-218 of SEQ ID NO:1, except that each amino acid corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine or is independently substituted with any other amino acid except tyrosine, from 0 to 2 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid.
6. The immunogenic composition of claim 1 , wherein the peptide comprises amino acids 1-222 of SEQ ID NO:1, except that each amino acid corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine or is independently substituted with any other amino acid except tyrosine, from 0 to 2 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid, and from 0 to 1 amino acids of amino acids 197-222 of SEQ ID NO: 1 is independently substituted with any other amino acid.
7. The immunogenic composition of claim 1 , wherein one or both of the amino acids corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine.
8. The immunogenic composition of claim 2 , wherein one or both of the amino acids corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine.
9. The immunogenic composition of claim 3 , wherein one or both of the amino acids corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine.
10. The immunogenic composition of claim 4 , wherein one or both of the amino acids corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine.
11. The immunogenic composition of claim 5 , wherein one or both of the amino acids corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine.
12. The immunogenic composition of claim 6 , wherein one or both of the amino acids corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine.
13. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 1 .
14. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 2 .
15. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 5 .
16. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 6 .
17. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 7 .
18. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 8 .
19. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 11 .
20. A method of vaccinating a mammalian subject against Borrelia burgdorferi , comprising administering to the subject the immunogenic composition of claim 12 .