IP Library › Granted Patent US 12,577,600
Granted Patent B2
US 12,577,600 · App. 18/481,401 · Granted Mar 17, 2026

Modification of RNA-related enzymes for enhanced production

Inventors: Bettina Strack-Logue (Lexington, MA); Frank DeRosa (Lexington, MA); Michael Heartlein (Lexington, MA)
Assignee: TRANSLATE BIO, INC.
C12P19/34C07K14/47C12N9/1241C12N15/62C12Y207/0705C07K2319/35C07K2319/95
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Quick Facts
Patent No.
US 12,577,600
App. No.
18/481,401
Granted
Mar 17, 2026
Kind
B2
Abstract

The present invention provides, among other things, methods and compositions for large-scale production of capped mRNA using SUMO-Guanylyl Transferase fusion protein.

Claims (6)

1 . A method of producing a capped RNA or RNA analog oligonucleotide, comprising transferring and methylating a guanylyl molecule to the 5′ end of the RNA or RNA analog oligonucleotide using a guanylyl transferase, wherein the guanylyl transferase comprises

i) a fusion protein comprising a small ubiquitin-like molecule (SUMO) protein covalently linked to guanylyl transferase large subunit, wherein the SUMO protein comprises SEQ ID NO: 1 and the guanylyl transferase large subunit comprises SEQ ID NO: 6, and

ii) a guanylyl transferase small subunit comprising SEQ ID NO: 7, thereby producing a capped RNA or RNA analog oligonucleotide.

2 . The method of claim 1 , wherein the fusion protein comprises, from the N-terminal to the C-terminal direction, SEQ ID NO: 1 fused to SEQ ID NO: 6.

3 . The method of claim 1 , wherein the guanylyl transferase has comparable phosphatase activity, guanylyl transferase activity and methylation activity relative to a wild-type guanylyl transferase protein.

4 . The method of claim 1 , wherein the RNA or RNA analog oligonucleotide is mRNA synthesized by in vitro transcription.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 17, 2026
From: STRACK-LOGUE, BETTINA; DEROSA, FRANK; HEARTLEIN, MICHAEL
To: SHIRE HUMAN GENETIC THERAPIES, INC.
Reel/Frame 073802/0399 →
Continuity (5)
Continuation 17217752 · Mar 30, 2021
Continuation 16165372 · Oct 19, 2018
Continuation 15294249 · Oct 14, 2016
Provisional Application 62241350 · Oct 14, 2015
Related Publication 20240175065A1 · May 30, 2024
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