IP Library › Granted Patent US 12,319,716
Granted Patent B2
US 12,319,716 · App. 18/595,330 · Granted Jun 3, 2025

Proteins toxic to Hemipteran insect species

Inventors: James A. Baum (Webster Groves, MO); Artem G. Evdokimov (Orchard Park, NY); Stanislaw Flasinski (Ballwin, MO); Farhad Moshiri (Chesterfield, MO); Timothy J. Rydel (St. Charles, MO); Eric J. Sturman (Wildwood, MO); Moritz von Rechenberg (Waltham, MA); Halong Vu (Arlington, MA); Andrew M. Wollacott (Boston, MA); Meiying Zheng (Wildwood, MO)
Assignee: Monsanto Technology LLC
C07K14/325A01N37/46C07K14/001C07K14/32C07K14/43577C12N15/8286Y02A40/146
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Quick Facts
Patent No.
US 12,319,716
App. No.
18/595,330
Granted
Jun 3, 2025
Kind
B2
Abstract

The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

Claims (25)

1. An insect inhibitory recombinant polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 12.

2. The insect inhibitory recombinant polypeptide of claim 1 , wherein the polypeptide exhibits inhibitory activity against an insect species of the order Hemiptera.

3. The insect inhibitory recombinant polypeptide of claim 2 , wherein the Hemipteran species is selected from the group consisting of a Lygus sp., an Emrasca sp., and an Amrasca sp.

4. The insect inhibitory recombinant polypeptide of claim 2 , wherein the Hemipteran species is selected from the group consisting of Lygus Hesperus, Lygus lineolaris , and Amrasca devastans.

5. A polynucleotide encoding the insect inhibitory recombinant polypeptide of claim 1 .

6. The polynucleotide of claim 5 , wherein the polynucleotide comprises the nucleotide sequence as set forth in SEQ ID NO: 11.

7. A host cell comprising the polynucleotide of claim 5 , wherein the host cell is selected from the group consisting of a bacterial host cell and a plant host cell.

8. The host cell of claim 7 , wherein the bacterial host cell is selected from the group consisting of Agrobacterium, Rhizobium, Bacillus, Escherichia, Pseudomonas , and Salmonella; and wherein the Bacillus species is a Bacillus thuringiensis , and the Escherichia is an Escherichia coli.

9. The host cell of claim 7 , wherein the plant host cell is selected from the group consisting of a monocot cell and a dicot cell.

10. An insect inhibitory composition comprising the insect inhibitory recombinant polypeptide of claim 1 .

11. The composition of claim 10 , wherein the composition is prepared by lyophilization, extraction, filtration, or centrifugation.

12. The insect inhibitory composition of claim 10 , further comprising at least one insect inhibitory agent different from the insect inhibitory recombinant polypeptide.

13. The insect inhibitory composition of claim 12 , wherein the at least one insect inhibitory agent is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, and an insect inhibitory chemistry.

14. The insect inhibitory composition of claim 12 , wherein the at least one insect inhibitory agent exhibits activity against one or more pest species of the order Lepidoptera, Coleoptera, Hemiptera, or Homoptera.

15. A seed comprising the polynucleotide of claim 5 .

16. The seed of claim 15 , wherein the polynucleotide comprises the sequence as set forth in SEQ ID NO: 11.

17. A method of controlling a Hemipteran pest, the method comprising presenting the Hemipteran pest with an inhibitory amount of the insect inhibitory recombinant polypeptide of claim 1 .

18. The method of claim 17 , wherein said presenting is via expressing the insect inhibitory recombinant polypeptide in a cotton plant.

19. A transgenic plant cell, plant, or plant part comprising the insect inhibitory recombinant polypeptide of claim 1 .

20. A method of controlling a Hemipteran pest, comprising exposing the Hemipteran pest to the transgenic plant cell, plant or plant part of claim 19 , wherein the plant cell, plant or plant part expresses a Hemipteran inhibitory amount of the insect inhibitory recombinant polypeptide.

21. A commodity product obtained from the plant cell, plant, or plant part of claim 19 , wherein the commodity product comprises a detectable amount of the insect inhibitory recombinant polypeptide, wherein the commodity product is selected from the group consisting of plant biomass, oil, meal, animal feed, flour, flakes, bran, lint, hulls, and processed seed.

22. A method of producing a seed, the method comprising:

a. planting at least one seed comprising a polynucleotide encoding the insect inhibitory recombinant polypeptide of claim 1 ;

b. growing at least one plant from the at least one seed; and

c. harvesting a seed from the at least one plant, wherein the harvested seed comprises the polynucleotide encoding the insect inhibitory recombinant polypeptide.

Continuity (9)
Division 17742215 · May 11, 2022
Continuation 17127310 · Dec 18, 2020
Continuation 16594713 · Oct 7, 2019
Continuation 16209501 · Dec 4, 2018
Continuation 15651727 · Jul 17, 2017
Division 15015957 · Feb 4, 2016
Continuation 13857196 · Apr 5, 2013
Provisional Application 61621436 · Apr 6, 2012
Related Publication 20240262872A1 · Aug 8, 2024
References Cited (54)
US 5723440A · Stockhoff et al. · 1998 [cited by applicant]
US 5885963A · Stockhoff et al. · 1999 [cited by applicant]
US 5942658A · Donovan et al. · 1999 [cited by applicant]
US 7473821B2 · Abad et al. · 2009 [cited by applicant]
US 7524810B1 · Schnepf · 2009 [cited by applicant]
US 7615686B2 · Miles et al. · 2009 [cited by applicant]
US 9713334B2 · Baum et al. · 2017 [cited by applicant]
US 11981709B2 · Baum · 2024 [cited by applicant]
US 12054522B2 · Baum et al. · 2024 [cited by applicant]
US 20060021087A1 · Baum et al. · 2006 [cited by applicant]
US 20060242732A1 · Carozzi et al. · 2006 [cited by applicant]
US 20080295207A1 · Baum et al. · 2008 [cited by applicant]
US 20100064394A1 · Baum et al. · 2010 [cited by applicant]
US 20100298207A1 · Sampson et al. · 2010 [cited by applicant]
US 20130269060A1 · Baum et al. · 2013 [cited by applicant]
US 20220356215A1 · Baum et al. · 2022 [cited by applicant]
EP 2834266B1 · 2019 [cited by applicant]
WO 19930014205A1 · 1993 [cited by applicant]
WO 19960039843A1 · 1996 [cited by applicant]
WO 20010071042A2 · 2001 [cited by applicant]
WO 20020078437A2 · 2002 [cited by applicant]
WO 20050110068A2 · 2005 [cited by applicant]
WO 20060107761A2 · 2006 [cited by applicant]
WO 20070027776A2 · 2007 [cited by applicant]
WO 20080134072A2 · 2008 [cited by applicant]
WO 20100025320A1 · 2010 [cited by applicant]
WO 20100099365A2 · 2010 [cited by applicant]
Baum et al., “Binary Toxins from Bacillus thuringiensis Active against the Western Corn Rootworm,” Diabrotica virgifera virgifera LeConte, Applied and Environmental Microbiology, 70(8):4889-4898 (2004). [cited by applicant]
Chan et al., “Unusual Amino Acid Determinants of Host Range in the Mtx2 Family of Mosquitocidal Toxins,” The Journal of Biological Chemistry, 271(24):14183-14187 (1996). [cited by applicant]
Chougule et al., “Toxins for Transgenic Resistance to Hemipteran Pests,” Toxins 4:405-429 (2012). [cited by applicant]
Correspondence from NCBI dated Sep. 24, 2010 re Date of First Public Release for DQ836184, 2 pages. [cited by applicant]
Crickmore et al., “Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins,” Microbiology and Molecular Biology Review, 62(3):807-813 (1998). [cited by applicant]
Donovan et al., “Characterization of Two Genes Encoding Bacillus thuringiensis Insecticidal Crystal Proteins Toxic to [cited by applicant]
EBI Accession No. GSP: ABB68459, “ [cited by applicant]
EMBL Accession No. DQ836184, Bacillus thuringiensis strain F14-1 Cry51 Aal (cry51Aa1) gene, complete CDs, http://srs.ebi.ac.us/srsbin/cgi-bin/wget?-e+[EMBL:DQ836184]+-newld, created Aug. 1, 2007, 2 pages. [cited by applicant]
Extended European Search Report dated Oct. 6, 2010, in European Patent Application No. 08754143.9, 6 pages. [cited by applicant]
Extended European Search Report dated Sep. 14, 2015, in European Patent Application No. 13772577.6, 7 pages. [cited by applicant]
GenBank Accession No. DQ836184, Bacillus thuringiensis strain F14-1 Cry51Aa1 (cry51Aa1) gene, complete cds, 1 page. Aug. 1, 2007, Web, Apr. 11, 2009 http://www.ncbi.nlm.nih.gov/nuccore/112253718. [cited by applicant]
Hofte et al. “Insecticidal Crystal Proteins of Bacillus thuringiensis,” Microbiological Reviews, American Society for Microbiology, 53(2):242-255 (1989). [cited by applicant]
Huang et al., “Microbial Control and Biotechnology Research on Bacillus thuringiensis in China,” Journal of Invertebrate Pathology, 95(3): 175-180 (2007). [cited by applicant]
International Search Report and Written Opinion dated Nov. 24, 2008, in International Application No. PCT/US2008/005542. [cited by applicant]
Lambert et al., “Novel Bacillus thuringiensis Insecticidal Crystal Protein with a Silent Activity against Coleopteran Larvae,” Applied and Environmental Microbiology, 58(8)2536-2542 (1992). [cited by applicant]
Liu et al., “New Gene from Nine Bacillus sphaericus Strains Encoding Highly Conserved 35.8-Kilodalton Mosquitocidal Toxins,” Applied and Environmental Microbiology, 62(6):2174-2176 (1996). [cited by applicant]
NCBI Accession No. D0836184, Bacillus thuringiensis strain F14-1 Cry51Aa1 (cry51Aa1) gene, obtained Oct. 1, 2010 from http://ncbi.nil.nih.gov/nuccore/112253718, 1 page. [cited by applicant]
NCBI Sample GenBank Record obtained Oct. 1, 2010 from http://www.ncbi.nilm.gov/Sitemap/samplerecord.html, 17 pages. [cited by applicant]
New England Biolabs, Random Primer 12, Jun. 2004, http://web.archive.org/web/20040619083054/http://www.neb.com/nebecomm/productS1256.asp, 1 page. [cited by applicant]
New England Biolabs, Random Primer 24, Jun. 2004, http://web.archive.org/web/20040619083054/http://www.neb.com/nebecomm/productS1256.asp, 1 page. [cited by applicant]
Revision history for NCBI Accession DQ836184, Bacillus thuringiensis strain F14-1 Cry51Aa1(crry51Aa1) gene, obtained on Oct. 1, 2010 from http://www.ncbi.nlm.nih.gov/sviewer/girevhist.cgi?val=DQ836184.1&log$=seq-view, 1… [cited by applicant]
Soberon et al., “Engineering Modified Bt toxins to Counter Insect Resistance,” Science, 31895856):1640-1642 (2007). [cited by applicant]
Thanabalu et al., “A Bacillus sphaericus Gene Encoding a Novel Type of Mosquitocidal Toxin of 31.8 kDa, Gene,” 170(1):85-89 (1996). [cited by applicant]
UniProt Accession No. A7IE5_BACTU, 1 page, accessed on Oct. 13, 2015 http://www.genome.jp/dbget-bin/www_bget?uniprot:A7IZR5_BACTU. [cited by applicant]
Vita et al., “Scorpion Toxins as Natural Scaffolds for Protein Engineering,” Proc. Natl. Acad. Sci. USA, 92:6404-6408 (1995). [cited by applicant]
Von Tersch et al., “Membrane-Permeabilizing Activities of Bacilllus thuringiensis Coleopteran-Active Toxin CryIIIB2 and CryIIIB2 Domain I Peptide,” Applied and Environmental Microbiology, 60(10):3711-3717 (1994). [cited by applicant]
Wellman-Desbiens, et al., “Development of a Bacillus thuringiensis-Based Assay on Lygus hesperus,” J. Economic Entomology, 98(5):1469-1479 (2005). [cited by applicant]